1.Larval aggregation on a burned human remain
Kumara, T.K. ; Abu Hassan, A. ; Che Salmah, M.R. ; Bhupinder, S.
Tropical Biomedicine 2012;29(1):197-199
Abstract. A burned human remain was found outdoor (5º 27’ N, 100º 16’ E) in Penang Island. The deceased was last seen alive on 23 April 2010 at 2230 h and was found burned on 24 April 2010 at 1920 h. Larval aggregation of second instar Chrysomya megacephala was observed on the chest of the deceased.
2.Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLC-MS/MS and its application in bioequivalence studies
Singh Bhupinder ; Lokhandae S. Rama ; Dwivedi Ashish ; Sharma Sandeep ; Dubey Naveen
Journal of Pharmaceutical Analysis 2014;(2):144-152
A validated ultra-performance liquid chromatography mass spectrometric method (UPLC-MS/MS) was used for the simultaneous quantitation of candesartan (CN) and hydrochlorothiazide (HCT) in human plasma. The analysis was performed on UPLC-MS/MS system using turbo ion spray interface. Negative ions were measured in multiple reaction monitoring (MRM) mode. The analytes were extracted using a liquid-liquid extraction (LLE) method by using 0.1 mL of plasma volume. The lower limit of quantitation for CN and HCT was 1.00 ng/mL whereas the upper limit of quantitation was 499.15 ng/mL and 601.61 ng/mL for CN and HCT respectively. CN d4 and HCT-13Cd2 were used as the internal standards for CN and HCT respectively. The chromatography was achieved within 2.0 min run time using a C18 Pheno-menex, Gemini NX (100 mm ~ 4.6 mm, 5 mm) column with organic mixture:buffer solution (80:20, v/v) at a flow rate of 0.800 mL/min. The method has been successfully applied to establish the bioequivalence of candesartan cilexetil (CNC) and HCT immediate release tablets with reference product in human subjects.