Objective To investigates the possible methodes for adult human Schwann cell culture and proliferation with F-12 medium which could be used on human body. Methods Nineteen human peripheral nerves were used in this study, 12 of them were cultured under F-12 medium and the other 7 nerves under standerd medium. The medium were changed every 2 days, and the nerve tissues were transfered to another plate when there were too many cells, after 6-8 times of transferring, most of the cells were Schwann cells and the nerve tissues were digested using none cell toxic collagenas. The cells were counted and evaluated the survival rate with Live-Dead kit S-100 protein immunostaining was used to assesse the Schwann cell purity. Results The survival rate of the cells were 85.54% and 86.93% , and the Schwann cell purity were 82.64% and 86.37% respectively, there were no difference between the two different medium, but we got more cells from F-12 medium than Standard medium, which was 14.2?10~7 and 5.9?10 respectively. Conclusion The F-12 medium could be used for human Schwann cell culture, meanwhile it had possible function on Schwann cell proliferation.