Study on some methods used to quantify berberine chloride showed that the UV measuring and volumetric titration methods did not express only amount of berberine chloride itself. It is necessary to apply high- performance liquid chromatography (HPLC) to achieve the optimal conditions for better splitting, higher accuracy…in order to apply more widely in testing products commonly in market with many components combined to berberine chloride. To find out optimal methods, which are suitable with condition of present some pharmaceutical units, it is necessary to experiment continuously methods such as UV measuring and volumetric titration,… using HPLC method to compare.
Berberine ; Tablets

Protoberberine alkaloids belong to the quaternary ammonium isoquinoline alkaloids, and are the main active ingredients in traditional Chinese herbal medicines, like Coptis chinensis. They have been widely used to treat such diseases as gastroenteritis, intestinal infections, and conjunctivitis. Studies have shown that structural modification of the protoberberine alkaloids could produce derivative compounds with new pharmacological effects and biological activities, but the transformation mechanism is not clear yet. This article mainly summarizes the researches on the biotransformation and structure modification of protoberberine alkaloids mainly based on berberine, so as to provide background basis and new ideas for studies relating to the mechanism of protoberberine alkaloids and the pharmacological activity and application of new compounds.
Alkaloids ; Berberine ; Berberine Alkaloids ; Biotransformation ; Coptis

To contribute to methods of dosage berberin hydrochlorid, this study proposed a new procedure to determine the quantitative content of berberine hydrochlorid in the pharmaceutical substances and their preparation: “Non-aqueous titration of berberine hydrochloride by potentiometry”.
Berberine ; Pharmaceutical Preparations

Berberine and Palmatine were extracted from the powdered Huong Lien pills with a mixture of methanol and hydrochloric acid (100:1) and subjected to HPLC to simultaneously determine the alkaloids. The HPLC technique was carried out on a Lichrosorb RP-8 (10 m, 250x4 mm) column using a UV detector at =345 nm and a mixture of 3,4g potassium dihydrogen phosphate and 1,7g sodium lauryl sulfate dissolved in 1000 ml of water-acetonitrile (1:1) as a mobile phase. The experimental results proved that the proposed HPLC method was rapid, specific, accurate and precise.
Berberine ; Medicine, Traditional ; Chromatography, High Pressure Liquid ; Biochemistry ; Berberine Alkaloids

Coptidis Rhizoma is a common Chinese medicinal in clinical practice,with the effects of clearing heat,drying dampness,purging fire,and removing toxin. All the medicinal plants of Coptis can be used for clinical treatment,but some species are endangered due to resource destruction and difficulty in planting. The dominant medicinal components in Coptidis Rhizoma are isoquinoline alkaloids. There are various methods for the analysis and detection of alkaloids,such as LC-MS,HPLC,and TLC,among which LC-MS is the most widely applied. Different plants of Coptis vary in the kind and content of alkaloids. C. chinensis,C. deltoidea,C. teeta,C. chinensis var. brevisepala,C. omeiensis,C. quinquefolia,and C. quinquesecta mainly contain berberine,palmatine,coptisine,jatrorrhizine,and columbamine,five effective alkaloid components. Plant isoquinoline alkaloids( PIAs) have strong pharmacological activity but are difficult to prepare. The application of synthetic biology of PIAs will be helpful for the clinical application of PIAs. This paper reviews the research progress on biological resources of Coptis species and structures of alkaloids as well as analysis methods and synthetic biology for isoquinoline alkaloids in the medicinal plants of Coptis in recent years,which will facilitate the protection of Coptis medicinal resources and the application and development of alkaloids.
Alkaloids ; Berberine ; Berberine Alkaloids ; Coptis ; Drugs, Chinese Herbal ; Isoquinolines ; Rhizome

One of the constituents of the oriental traditional pill named Balok was Tuber Stephaniae rotundae, the active principle of which was rotundine. The test solutions used for the analysis of rotundine were standardized after ISO/IEC 17027 and GLP, the equipment was adjusted after ISO/IEC 17025. The proposed HPLC technique was proved to be appropriate and stable for the quantitative determination of rotundine. The examined concentration showed the linear correlation between the rotundine concentration and the pic surface. The reproducibility of the method was high, with RSD = 0.98%. The accuracy rate of the method was 100.01%, with RSD = 2.0%.
Chromatography, High Pressure Liquid ; Berberine Alkaloids

An HPLC method has been established to determine l-tetrahydropalmatine (rotundine) extracted by chloroform from powders of Stephania viridiflavens, S. dielsiana and Stephania sp. (Menispermaceae). The technique was carried out on an RP-18 (250 x 4.0 mm; 5 m) column with a diode array detector or UV detector at  = 283 nm, using a mixture of 0.05 M potassium dihydrogen phosphate, acetonitrile and triethylamine (62.5 : 37.5 : 0.5) adjusted to pH 4.0 by concentrated phosphoric acid as a mobile phase. The HPLC method proved accurate, specise and sensitive. The results show that differences exist in the content of l-tetrahydropalmatine between species.
Chromatography, High Pressure Liquid ; Berberine Alkaloids

OBJECTIVETo study the alkaloid constituents of Corydalis adunca.

METHODThe constituents were isolated on silica gel column and their structures were elucidated by IR, NMR, MS data.

RESULTFour alkaloid compounds were isolated from alcohol extract of the herb of C. adunca, and identified as tetrahydropalmatine, 1, 2, 3, 4-tetrahydro-6-methoxy-1-(4'-methoxyphenyl) methyl-7-isoquinolinol, coptisine and palmatine.

CONCLUSIONFour compounds were isolated from C. adunca for the first time.

Berberine ; analogs & derivatives ; chemistry ; isolation & purification ; Berberine Alkaloids ; chemistry ; isolation & purification ; Corydalis ; chemistry ; Plants, Medicinal ; chemistry

OBJECTIVETo study the absorption characteristics of alkaloids in Coptis chinensis in rat intestinal.

METHODIn situ single-pass perfused rat intestinal model was used and the concentrations of berberine and palmatine were determined by HPLC, the drug absorption rate constant (K(a)) and apparent absorption coefficient (P(app)) were tested to evaluate their absorption characteristics.

RESULTThe absorption of berberine and palmatine in different regions of rat intestinals was significantly different. From bigness to smallness, the P(app) value of berberine at 50 mg x L(-1) were ileum, duodenum, jejunum and colon, of palmatine at 50 mg x L(-1) were ileum, colon, jejunum and duodenum. The concentration of berberine and palmatine had distinctive effect on the K(a) value of berberine and palmatine were ordered from larger to little as in Wujiwan compatibility, Coptis chinensis extractive and single compound.

CONCLUSIONThe berberine and palmatine were absroped at all regions of rat intestinal. The absorption characteristics of 2 alkaloids were not passive diffusion. The other ingredients in Wujiwan compatibility and Coptis chinensis extractive could promote the absorption of alkaloids.

Alkaloids ; pharmacokinetics ; Animals ; Berberine ; pharmacokinetics ; Berberine Alkaloids ; pharmacokinetics ; Coptis ; Intestinal Absorption ; Male ; Perfusion ; Rats ; Rats, Wistar

Based on the concept of quality by design(QbD), the Box-Behnken design-response surface methodology combined with standard relation(SR) and analytic hierarchy process(AHP)-entropy weight method(EWM) was applied to optimize the extraction process of the classic prescription Yihuang Decoction. The content of geniposidic acid, phellodendrine hydrochloride, and berberine hydrochloride in Yihuang Decoction, the extract yield, and fingerprint similarity were used as the critical quality attributes(CQAs) of the extraction process. The extraction time, water addition, and extraction times were used as the critical process parameters(CPPs). After determining the levels of each factor and level through single-factor experiments, response surface experiments were designed according to the Box-Behnken principle, and the experimental results were analyzed. The SR between each sample and the reference sample under various evaluation indicators of different extraction parameters was calculated. The weights of the five evaluation indicators were determined using AHP-EWM, followed by comprehensive evaluation. A function model between CPPs and CQAs characterized by comprehensive scores was established to predict the optimal extraction process parameters. In the final comprehensive weight coefficients, the yield rate accounted for 43.1%, and the content of berberine hydrochloride, phellodendrine hydrochloride, and geniposidic acid accounted for 35.1%, 6.3%, and 15.5%, respectively. After comprehensive score analysis with SR, the established second-order polynomial model was statistically significant(P<0.01, and the lack of fit was not significant). The predicted optimal extraction conditions for Yihuang Decoction were determined as follows: 8-fold volume of water, extraction time of 1.5 h, and extraction once. The mean comprehensive score of the validation experiment was 85.77, with an RSD of 0.99%, and it met the quality control stan-dards for the reference sample of Yihuang Decoction. The results indicate that the optimized extraction process for Yihuang Decoction is stable and reliable, and the water extract is close in quality attributes to the reference sample. This can serve as a foundation for the research and development of granules in the future. Box-Behnken design-response surface methodology combined with SR and AHP-EWM can provide references for the modern extraction process research of other classic prescriptions.
Drugs, Chinese Herbal ; Analytic Hierarchy Process ; Berberine ; Entropy ; Water