OBJECTIVE To observe and compare the effects of conjunctival sac rinse of povidone iodine solution with sodium chloride solution.METHODS Eighty patients with senile cataract were randomly divided into 2 groups(group A and group B).Swab samples were taken for bacterial cultures from each patients of both groups before and 24 hours after application of 0.3% ofloxacin eye drops.Povidone iodine solution was used to rinse the conjunctival sac before cataract surgery in group A while 0.9% sodium chloride solution was used in group B,and swab samples were also taken for bacterial cultures.RESULTS Positive rates of the bacterial cultures before and after topical ofloxacin application were 58.8% and 27.5%,respectively.Positive rates of the bacterial cultures after conjunctival sac rinse with povidone iodine and sodium chloride were 5.0% and 20.0%,respectively.CONCLUSIONS Conjunctival sac rinse with povidone iodine solution should be taken as a routine procedure before intraocular surgery to decrease the risk of postoperative infections.

Objective:To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SV-HUC-1) and the pathogenesis of ketamine-associated cystitis. Methods:SV-HUC-1 cells were cultured under various concentrations of ketamine and different time. Flow cytometry was used to analyze the rate of cell apoptosis. hTe protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot. Results:Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. hTe expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was signiifcantly higher. hTe protein level of pro-caspase-3 was signiifcantly lower, and that of cleaved caspase-3 was signiifcantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05). Conclusion:Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.

Objective To understand mental health of young nurses and coping style, to explore the influence about relaxation training to their mental health. Methods A total of 90 nurses within five years work experience were randomly divided into stretch-release relaxation, cognitive relaxation training group and non-intervention group. Do a survey by symptom checklist-90 ( SCL-90 ) and simplified coping style questionnaire ( SCSQ) before and after training. Results The total scores of both two relaxation training groups ( SCL-90 ) significantly decreased after six months training to (142. 54 ± 26. 86) and (142. 51 ± 25. 96), and it had statistical significance comparing with non-intervention group (P<0. 01); the score of positive coping style in nurses was statistically significant after training (33. 62 ± 4. 55) (P<0. 01). Conclusions Relaxation training can enhance mental health of young nurses and guide nurses to adopt positive coping style, lower negative emotions, so it is worthy of applying and recommending in paramedics.