Objective To evaluate the diagnosis significans of histopathological observation combined with tumor markers detec-tion in gastric cancer.Methods To retrospectively review the pathological data and serum levels of tumor markers of 36 gastric cancer patients(gastric cancer group)and 41 patients with benign gastric disease(gastric disease group),and to compare with tumor marker detection levels of 30 healthy people(healthy control group)and analyze the differences.Results The serum levels of tumor markers in gastric cancer group were obviously higher than those in healthy control group and gastric disease group(P <0.05).The early diagnosed rate for gastric cancer was 55.6% by histopathological observation,which increased to 91.7% when combined with tumor markers detection.And the difference was significant(P <0.05 ).Conclusion Histopathological observation combined with tumor markers detection could improve the diagnosis sensitivity and accuracy in gastric cancers.