Objective:To compare the effects of Zn~(2+)onthe P2X receptor-mediated,ATP-induced currents in neurons separated from rat superior cervical ganglion(SCG),nodose ganglion(NG),and otic ganglion(OTG).Methods: Whole-cell patch clamp recording technique was used to study the regulatory effects of Zn~(2+) on ATP/??-me ATP-induced currents in the above 3 ganlglion neurons.Results: All SCG neurons responded to ATP with a sustained current,while no neurons responded to ??-me ATP;Zn~(2+) potentiated ATP-induced sustained currents to(1 442?34)% of the original value.All NG neurons responded to ATP and ??-me ATP with a similar sustained current;coapplication of Zn~(2+)(10 ?mol/L) potentiated their responses to(180?12)% and(262?28)%,respectively.All OTG neurons responded to both ATP and ??-me ATP with a sustained current.Coapplication of Zn~(2+)(10 ? mol/L) did not significantly potentiate the sustained currents induced by 10 ?mol/L ATP,but when ATP was at 30 ?mol/L,Zn~(2+)(10-100 ?mol/L) inhibited ATP-induced sustained currents in a dose dependent manner.If TNP-ATP(100 nmol/L) was first used to inhibit ATP-induced current to(26?2)% of the original value,Zn~(2+) at 10 ?mol/L potentiated the inhibited current to(127?9)% of its original value.Coapplication of Zn~(2+)(10 ?mol/L) potentiated ??-me ATP-induced currents to(146?5)% of the control.Zn~(2+)(300 ?mol/L) had no effect on ?_(on) and ?_(off) of ATP-and ??-me ATP-induced(30 ?mol/L) currents in OTG neurons.Conclusion:(1) Zn~(2+) is an allosteric modulator of P2X_(2) and P2X_(2/3) receptors in SCG and NG neurons and can potentiate the currents they induced.(2)The predominant receptor subtypes in OTG appear to be homomeric P2X_(2/3) and a little P2X_(2).Zn~(2+) has an inhibitory effect on the ATP-induced currents in OTG neurons,suggesting some novel members of the P2X purinoceptor exist in these neurons.

Objective: To compare the effects of Zn2+ on the P2X receptor-mediated, ATP-induced currents in neurons separated from rat superior cervical ganglion (SCG), nodose ganglion (NG), and otic ganglion (OTG). Methods: Whole-cell patch clamp recording technique was used to study the regulatory effects of Zn2+ on ATP/αβ-me ATP-induced currents in the above 3 ganlglion neurons. Results: All SCG neurons responded to ATP with a sustained current, while no neurons responded to αβ-me ATP; Zn2+ potentiated ATP-induced sustained currents to (1442±34)% of the original value. All NG neurons responded to ATP and αβ-me ATP with a similar sustained current; coapplication of Zn2+ (10 μmol/L) potentiated their responses to (180±12)% and (262±28)%, respectively. All OTG neurons responded to both ATP and αβ-me ATP with a sustained current. Coapplication of Zn2+ (10 μ mol/L) did not significantly potentiate the sustained currents induced by 10 μmol/L ATP, but when ATP was at 30 μmol/L, Zn2+ (10-100 μmol/L) inhibited ATP-induced sustained currents in a dose dependent manner. If TNP-ATP (100 nmol/L) was first used to inhibit ATP-induced current to (26±2)% of the original value, Zn2+ at 10 μmol/L potentiated the inhibited current to (127±9)% of its original value. Coapplication of Zn2+ (10 μmol/L) potentiated αβ-me ATP-induced currents to (146±5)% of the control. Zn2+ (300 μmol/L) had no effect on τon and τoff of ATP- and αβ-me ATP-induced (30 μmol/L) currents in OTG neurons. Conclusion: (1) Zn2+ is an allosteric modulator of P2X2 and P2X2/3 receptors in SCG and NG neurons and can potentiate the currents they induced. (2) The predominant receptor subtypes in OTG appear to be homomeric P2X2/3 and a little P2X2. Zn2+ has an inhibitory effect on the ATP-induced currents in OTG neurons, suggesting some novel members of the P2X purinoceptor exist in these neurons.

Adult ; China ; epidemiology ; Extraction and Processing Industry ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Occupational Exposure ; Pneumoconiosis ; epidemiology ; Young Adult

Serum CA19-9 is one of the markers most frequently detected in the diagnosis and biliopancreatic diseases.The levels of CA19-9 is important for the diagnosis,treatment and prognosis of biliopancreatic diseases.With the development of medical technology,the comprehension of the value of serum CA19-9 is more and more thoroughly.The benign and malignant diseases of biliopancreatic systems can be differentiated by the variation of the values of serum CA19-9,which has high sensitivity in malignant tumors.Serum CA19-9 is one of the independent indicators for evaluation of resectability of tumors and the survival time and life quality of the patients.In this article,the significance of serum CA19-9 in the diagnosis,treatment and prognosis evaluation for biliopancreatic diseases were reviewed.

Objective To investigate the effects of valsartan on the expression of acyl-coenzyme A: cholesterol acyltransferase-1(ACAT-1) and peroxisome proliferator activated receptor-gamma(PPAR-?) in atherosclerotic plaques on rabbit aortic wall.Methods Twenty-four male Japanese white rabbits were randomly assigned into three groups(8 each): control group,valsartan group and high cholesterol feeding group.All rabbits were fed according to the experimental protocol for 12 weeks.Blood samples were taken from vein for measurement of serum lipids.The ratio of intima/media thickness of the aorta was measured.ACAT-1 mRNA/protein and PPAR-? mRNA/protein were determined by reverse transcription polymerase chain reaction(RT-PCR) and Western blotting,respectively.Results After 12 weeks,the levels of serum total cholesterol(TC),triglyeride(TG) and low density lipoprotein-cholesterol(LDL-C) in valsartan group and cholesterol group were significantly higher than those in control group(P0.05).The intima thickness and the ratio of intima/media in carotid arteries in cholesterol group were significantly higher than those in control group and valsartan group(P

Objective To investigate the relationship between the mutation of the exon 4 of leptin receptor gene(4Lys 109 Arg)and left ventricular function and renal hemodynamics in patients with hypertension.Methods From December 2005 to December 2006,digoxin marked primer enzyme coloration technique was used to detect the 4Lys 109 Arg polymorphism.The color Doppler echocardiography was used to detect the left ventricular function and the renal hemodynamics.The study covered 90 patients with hypertension (hypertension group)and 52 controls (control group).Results The frequencies of 4Lys109Arg in hypertension group and control group had no significant difference.The hypertension with GG genotype was significant difference in left ventrieular ejection fraction(LVEF),E/A,left ventrieular fractional shortening(LVFS),resistance index(BI),pulsatility index(PI)(P<0.05).The hypertension with GA genotype had significant difference in LVEF,E/A,LVFS(P<0.05).In hypertension group,LVEF,E/A,LVFS.RI and PI had significant difference between GG and AA genotype(P<0.05).In control goup,RI had significant difference between GG and AA genotype(0.65 ±0.02 vs 0.63±0.02,P<0.05).Conclusion The renal hemodynamics and the left ventricular function are associated with 4Lys109Arg polymorphism.

Objective To discuss the establishment and application of PACS of our hospital, and its significance for the digitization of the radiology department. Methods PACS was adopted to store and transmission of images by CR, CT, MRI, DSA. The radiologist used PACS workstation to perform post-processing of the images. Results With PACS, filmless viewing, improved diagnosis quality, accelerated generation of diagnosis report could all be implemented. Conclusion With the application of PACS to digital and filmless imaging, the patient service by the hospital can be highly improved.

The digitallization of field medical equipment for the modern troop is an important sign on military logistics modernization.The use of CR technology on the existing field X-ray equipment for digitallization upgrading can be combined with CR and PASC system,which is an effective channel on achieving digitalization of our military field X-ray image system.So the design plan of field CR and micro-PACS system combination is advanced.

Objective To investigate the relation of serum leptin level in senile depressive patients with type 2 diabetes and polymorphisms of leptin receptor gene 3057 G/A.Methods 10 senile depressive patients with type 2 diabetes and 47 senile depressive patients without type 2 diabetes were selected for the study.Leptin levels were determined by radioimmunoassay.Genomitic DNA were obtained by phenol/chloroform method.Leptin receptor gene 3057 G/A polymorphisms and allele frequencies were determined with the polymerase chain reaction and restricted fragment length polymorphism assay.Results (1) The differences of leptin receptor genotypes and allele distribution in the 3 groups were not found (all P ＞ 0.05).(2) Serum leptin level in senile depressive patients with type 2 diabetes and without type 2 diabetic patients ((41.65 ± 28.19) ng/ml,(25.56 ± 21.04) ng/ml) were significantly higher than the normal people ((10.84 ± 9.16) ng/ml) (all P ＜ 0.05).Serum leptin level in senile depressive patients with type 2 diabetes were significantly higher than without type 2 diabetic patients(P＜ 0.05).(3) The serum leptin level in genotype AA senile depressive patients with type 2 diabetes were significantly higher than genotype AA patients without type 2 diabetes.The differences of the serum leptin levels in genotype AA senile depressive patients without type 2 diabetes and genotype AG/GG patients without type 2 diabetes were not found(P ＞ 0.05).The differences of the serum leptin level in genotype AA and genotype AG/GG in normal groups were not found (P＞ 0.05).Conclusion Senile depressive patients have higher serum leptin level.The serum leptin level is higher,especially with type 2 diabetes.Serum leptin level is related to type 2 diabetes.Serum leptin level is not related to the polymorphisms of leptin receptor gene 3057 G/A.Type 2 diabetes are not related to polymorphisms of leptin receptor gene 3057 G/A.

OBJECTIVE:To determine the equilibrium solubility of albendazole(ABZ)and its apparent oil-water partition coef-ficient. METHODS:The equilibrium solubility of ABZ in various solutions was determined at 37 ℃ by HPLC and saturation solu-bility method,including water,7 kinds of common organic solvents with different polarities(methanol,ethanol,etc.),organic ac-id(oleic acid,glacial acetic acid,lactic acid,formic acid),hydrochloric acid of pH 1.2,phosphate buffer solution(PBS)of dif-ferent pHs(2.0-7.8)and 6 kinds of common surfactants with mass concentrations of 10,50 and 100 mg/ml(polysorbate 80,polox-amer,etc.). The apparent oil-water partition coefficient(P)was calculated with the concentration ratio of ABZ in oil phase(N-oc-tyl alcohol)and water phase(water and PBS of different pHs)after partition equilibrium. RESULTS:The equilibrium solubility of ABZ reached(0.26±0.02)μg/ml in water,with the lgP of 3.66±0.01. The equilibrium solubility of ABZ in common organic sol-vents and organic acids was higher than in water. The higher the polarity of the organic solvent was and the weaker of organic acid was,the weaker of its ability to solubilize ABZ would be. The equilibrium solubility of ABZ was higher in the medium of pH 1.2-2.5 than in that of pH 5.0-7.8. The ability of the surfactant to solubilize ABZ was related to its type,and the higher of the mass concentration of the surfactant was,the stronger of its ability to solubilize ABZ became. lgP was less than 1.6 at pH 1.2-2.0 and changed little at pH 5.0-7.8 [(3.71 ± 0.26)-(3.68 ± 0.26)]. CONCLUSIONS:ABZ is insoluble in water. Its equilibrium solubility demonstrates a negative correlation with the polarity of the organic solvent and a positive correlation with the acidity of the organic acid and with the mass concentration of the surfactant. It has higher water solubility and lower lipid solubility in a strong acidic en-vironment,and higher lipid solubility and weaker water solubility in weak acidic,weak basic and neutral environments.