BACKGROUND: A recent day is one of a rare drug plant, which use in traditional medicine a long time ago. Therefore based on nature resource of traditional medicine, on the base of evaluating pharmacological and biological action to develop a new drug of plant origin is important not only for treatment, but also has a economic significance. This plant has profound medicinal use and is a proved antipyretic, analgesic anti inflammatory and anti-cancer. No detail report was found in literature to evaluate renal damage experimentally in rats. The present study was hence designed to determine protective effect (Saposhnikovia divaricate (Turcz) Schischk) kanamycin-induced nephrotoxicy in rats. In addition, we attempted to test and compare the possible action of Saposhnikovia divaricate (Turcz) Schischk) kanamycin-induced nephrotoxicity in rats. MATERIALS AND METHODS: Experimental animals A dry extract of SDS is root was prepared by the lyophilization method and used in the study. Threemonth old Wistar albino rats of either sex weighing 150-250 g were used for the study. The animals were placed at random and allocated to treatment groups in polypropylene cages with paddy husk as bedding. Animals were housed at a temperature of 24 ± 2⁰C and relative humidity of 30-70%. A 12/12 h light and dark cycle was followed. All animals were fed on standard balanced diet and provided with water ad libitum. All the experimental procedures and protocols used in the study were reviewed and approved by the Bio-Medical Ethical Committee of Mongolian National University of Mongolia. Biochemical estimation At the end of experimental period, rats were anaesthetized with ether. The pathological model of kidney we use lipid peroxidation in the process of kidney inflammation kidney tissue, blood serum, erythrocyte MDA of membrane amount on 3, 7, 14 day by using spectrophotometer apparatus of “Shimadzu” firm of Japan and measured absorption at 535 nm. Statistical Analysis: Statistical analysis of data was performed by SPSS 16.0 program and analyzed statistically using criteria of Student t test. RESULTS: When we studied effect of extract of SDS preparation to condition of lipid peroxidation activation in the process of kidney inflammation by the indices of MDA which contains in blood plasma and erythrocyte membrane, MDA amount in renal tissue, amount of MDA decreased and it showed effect of decreasing lipid peroxidation MDA of plasma. CONCLUSION: The extract of Saposhnikoviadivaricata (Turcz) Schischk) has action of protecting kidney and decreasing lipid per oxidation.

The Ellipin, derived from bovine liver, is a newly developed agent containing several important fattyacids and may have anticancer effects on cancer cell in vitro culture. This Ellipin preparation containmany PUFAs and those are important molecules for membrane order and function; they can modifyinflammation-inducible cytokines production, eicosanoid production, plasma triacylglycerol synthesisand gene expression. Recent studies suggest that anti-proliferation effect on in vitro cancerous cell linese.g. J62, Raji, Hela, K582, and in vivo it minimized and decreased the growth of U14 cervical cancer inmice and suppressed the growth of its S37 sarcoma cells, increased the area of dead cancer cells, anddecreased the angiogenesis. Also it can be cancer chemopreventive and auxiliary agents for cancertherapy. The Ellipin could alter cancer growth influencing cell replication, cell cycle, and cell death.However, the effects of the Ellipin function on tumor growth in vivo have not been studied completlyand the mechanisms remain unclear. The question that remains to be answered is how, which pathwayEllipin can affect so many molecular ways inside cancer cell. We hypothesize that Ellipin’s mixture ofunsatured fatty acids effect inductor molecules in cancer gene which inhibits a repressor protein bindingto promotor and synthesis signaling molecules (caspase protein, TNF- ¹) in liver cancer cell. Also italter membrane stability and modifing cellular signalling.

IntroductionSea buckthorn is known to be one of the vitamin-rich berries in the plant kingdom and has beencredentialed as highly valued for healthy living, improving well-being, enhancing lifestyle, and preventingthe disease. Widely recognized in Northern regions of Europe and Asia, Hippophae rhamnodes hasbeen used medicinally for thousands of years. Both of seed and pulp oil have tocopherols, carotinoids,as well as ω-3 and ω-6 fatty acid families. The seed oil is highly unsaturated, with proportions of linoleic(C 18:2n-6) and α-linolenic (C18:3n-3) acids between 30-40% and 20-35%, respectively, whereas thepulp oil is more saturated containing high amounts of palmitoleic (C16:1n-7, 16-54%) and palmitic acids(16:0,17-47%). Many researchers including Laagan B., Avdai Ch., Tsendeekhuu Ts., Vernik S. R.,Jamyansan Y., Badamkhand D., Odonmajig P have determined content of berry, fatty acid compositionof sea buckthorn pulp and seed oil since 1970 in Mongolia. However, total fat and fatty acids compositionof Hippophae rhamnodes general is known, limited reports exist dealing with comparative differencesin fatty acid composition of Hippophae rhamnodes grown in Mongolia. Therefore, we analyzed thecomposition of fatty acids pulp oil and seed oil in sea buckthorn grown in Mongolia.Materials and MethodsPulp and seed oil were produced in Monos Food Co Ltd., and fatty acid methyl ester were analyzedusing Agilent Packard Gas Chromatograph (GC) (Model HP-6890 Agilent Packard) with mass-spectrumdetector (Model HP MSD 5973N).ResultThe results indicated that unsaturated fatty acids in the seed oil (85.4%) and in pulp oil (62.5%) arehigher than saturated fatty acids of seed oil (14.6%) and pulp oil (37.1%) respectively. The mostimportant factor defining the nutritional value of oil is ratio of unsaturated fatty acids present in oil. Theseed oil contains palmitic acid (10.8%), oleic acid (20.3%), limoleic acid (42.9%), α-linolenic acid (5.6%),Eicosadienoic acid (14.7%). Pulp oil of Hippophae rhamnodes has palmitic acid (35.4%), palmitoleicacid (38.5%), oleic acid (including Vaccenic acid) (6.6%) and linoleic acid (10.4%). The palmitic acid andpalmitoleic acid ratio in pulp oil was more than in seed oil. While oleic acid, linoleic acid and α-linolenicacid in seed oil have a higher ratio than that of pulp oil. It shows that unsaturated fatty acids in seed oilare much higher than pulp oil.ConclusionUnsaturated fatty acids in the pulp oil contain 62.5%, including essential fatty acid ω-3, 6, 9 (18.2%);it is content of 29.12% in unsaturated fatty acids. While, unsaturated fatty acids of seed oil contains85.4% in total fatty acid and essential fatty acid contains 96.72% in unsaturated fatty acid. It can thus beconcluded that seed oil is better than pulp oil because the former contains essential fatty acid.

Background In the present work, a reverse – phase high –performance liquid chromatographic procedure has been developed for the determination of vitamin E in cereals. The sample treatment was done by Sohhlet extraction procedure with a water bath. Afterwards, vitamin E was analyzed by HPLC on a Discovery C 18 (250 x 4.6 mm, 5 mm) analytical column, using a solution of methanol: water (95:5, v/v) as mobile phase. The UV-Vis detection of vitamin was made at 210 nm for all samples and fl ow rate was adjusted at 0.8 ml.min-1. Among grain-cereal products, average content of vitamin E were determined in kamut, špaldove kernotto and špalda loupana, namely 1.19, 1.19 and 1.30 mg.100g-1. Keywords: vitamin E, HPLC, cereal INTRODUCTION Vitamin E is a fat soluble vitamin, being well presented in vegetables, fruits, seeds, nuts (Bramley et al., 2000). Cereals are one of the important sources of lipids as well as a good source of vitamin E. Several studies have been investigated that presences of numerous antioxidant compounds in two specifi c food groups, the carotenoids and the vitamin E contributes signifi cantly in disease prevention ( Palozza & Krinsky, 1992, Halliwell et al.,1995, Andlauer & Fürst, 1998 ). Biological activities of vitamin E vitamers generally believed to its antioxidant action by tocopherols. Specially, α-tocopherol has been labeled as most effi cient antioxidant for lipid peroxidation in biological membranes (Traber & Atkinson, 2007). Vitamin E is not synthesized within a human body, and must be supplied in the diet. More data on vitamin E contents in cereals and other foodstuffs are needed, however, since they have been investigated with modern, reliable analytical methods in a few studies (Panfi li, 2003, Sundl et al., 2007). Due to the reason many researche have been performed to develop a simple and sensitive determination method for vitamin E and other fat soluble vitamins in foods. Several HPLC methods for determination of vitamin E are described in the literature, as well as methods its vitamers simultaneous determination (Lang et al., 1992, Luque-Garcia, 2001, Escriva´ et al., 2002). The aim of this study was to develop a normal phase HPLC method for a fast and reliable determination of vitamin E of cereals. Therefore, it was to investigate the precision of calculatedvitamin E content of cereals. MATERIAL AND METHODS Chemicals. D, L-α-tocoferol and - were from Fluka (Sigma-Aldrich Co, Germany). Methanol (purity: 99.8%) was obtained from Sigma-Aldrich (Germany). All other reagents were analytical grade and were purchased from Petr Lukes (Uhersky Brod, Czech Republic). Samples. For this analysis were chosen following cereals grown in organic farming: Kamut, Špaldove kernotto, Grünkern and Špalda loupaná. Individual samples were delivered directly by the manufacturer PRO-BIO, Ltd., (Staré Město Sněžníkem, Czech Republic). Cereals products stored in the original container in a dark place at room temperature and not more than 4 months after packing.

BackgroundPanax ginseng is one of the most important medicinal plants in Asia. Triterpene saponins(ginsenosides) are the main bioactive compounds in P.ginseng. The present study investigated thegrowth characteristics of ginsenoside content in the leaves and roots of Panax ginseng at differentgrowth stages (from 1 and 4 years). Analysis was P.ginseng leaves and roots indicated the presenceof two ginsenosides (Rg1 and Rb1) content of Rg1 was higher than Rb1.Aim: The main aim of this study was determine by high performance liquid chromatography (HPLC)ginsenoside Rg1 an Rb1 of P.ginseng grown in Mongolia.Materials and MethodsLeaves and roots of different ages of P.ginseng were collected at October of 2015 in field of Umnugoviprovince, Mongolia. Collected samples were dried and powdered. Samples were extracted with70% EtOH, water saturated butanol and methanol. The extract was filtrated through filter paper(whatman No.42) and evaporated vacuum rotor. The evaporated extract was resuspended with theMethanol. It stored in room temperature and resuspended mobile phase use for HPLC analysis.The two ginsenosides were analyzed using HPLC system of a model (DGU-20A3r Shimadzu) andcolumn was Octadecylsilane 5μm I-150mm, D-4.6mm, detector was UV 204 nm. The sample wasinjected (20μl) and applied gradient elution was as follows British pharmacopeia.ResultsGinsenosides levels were much higher in the 4 ages roots compared with the 1 ages roots.Ginsenoside amounts in P.ginseng organs changed depending on the specific time during thevegetation season the samples were taken. This study found that the highest content of thesemetabolites-2.082% (butanol extract) occurred in the roots. Our study was independently of thevegetation season. These were Rb1 and Rg1, with values Rg1 was 0.7-2.082% and Rb1 was 0.002-0.03%.ConclusionGinsenosides are generally distributed throughout all the parts of the ginseng plant. We found thatthe highest Ginsenoside Rg1 content accumulated during the first year of growth then decreaseduntil four year.

BACKGROUND. UNIFACE 4.0 program has started implementing of UNIFACE 4.0 program for training since 2004 at MNUMS. In 2008, registration and information of the six branch schools, MNUMS had been transformed into electronic forms. Since 2010, the electronic form and an integrated information environment has been reached all schools equally. The business Plan of MNUMS is to become “Open School” under the strategic goal, and UNIMIS is being introduced and implementing by the Presidential ordinance A/55, on May 06, 2015. OBJECTIVE. To analyze the implementation of the university management information system and determine future trends and approaches RESULTS. In the survey, 7.89% of the subjects were the first group (teachers) responded that it was not appropriate to continue using UNIMIS for training, were below the average, 39.47% of the teachers responded that it was appropriate in some ways and 52.68% said that they would be better useful than average. 15.76% of the subjects were the second group (students) surveyed were moderately unsuitable, 43.15% responded that they were some appropriately, and 41.08% said that they were moderate or higher useful for them. To fulfill consistently the development of UNIMIS in the near future is required to enhance the training specific characteristics and the period, which is the foundation for further application of this program. Over 90% of the teachers who in the study answered that they only used the grades putting into the UNIMIS, the remaining percentages were asked for using students’ self-study and taking a survey. There are many modules in the UNIMIS program, but they are mostly used for students’ grades in it. For teachers, it may be due to a lack knowledge of about UNIMIS.The foundation for further application of this program is required to fulfill and develop UNIMIS permanently with the training specific characteristics and the period. CONCLUSION. In UNIMIS, there are many issues; inadequate for registration and information (data), incomplete of system usage and applications, not well educated, unsuffiency and a lack of technical and technological safety.

IntroductionA hangover is the experience of various unpleasant physiological and psychological effects followingconsumption of alcohol beverages, which can last for more than 24 hours. Common symptoms ofhangover are headache, gastrointestinal complaints, sweating, hyper-excitability, dry mouth, anorexia,diarrhea, dizziness, fatigue and vertigo. Alcohol or ethanol gets metabolized to an intermediate product,acetaldehyde, by the enzyme alcohol dehydrogenase (ADH), and then acetaldehyde is converted toacetate by a second enzyme aldehyde dehydrogenase (ALDH). Acetaldehyde causes toxic effects,such as high pulse, rate, sweating and vomiting. In most people, ALDH metabolizes acetaldehydequickly and effi ciently, so that this intermediate metabolite does not accumulate in high concentrations.Many treatments are described to prevent hangover, shorten its duration, and reduce the severity of itssymptoms, including innumerable folk remedies and recommendations.GoalThis study was conducted to investigate whether anti-hangover preparation has a protective effectagainst acute alcohol induced hangover in Wistar rats.Materials and MethodsMale and female Wistar line rats, weighing 180-210g were used for hangover model or ethanolmetabolism experiment. Rats were administered orally ethanol as 38% aqueous solution with feedingneedle, 1 ml/200g body weight. The anti-hangover preparation was administered 1 hour before ethanolconsumption. Blood was collected from the tail vein for the measurement of serum acetate andacetaldehyde at just before and 8, 16, 24 hour after ethanol administration.Statistical analysis: All value expressed as mean S.E obtained from n number of experiments.ResultFrom this study results summarize that the anti-hangover preparations decreased blood serum acetateand acetaldehyde levels as compared to control. Anti-hangover preparations enhanced acetaldehydeand acetate metabolism.Conclusion: These fi ndings indicate that anti-hangover preparations may exert benefi cial role inthe treatment of alcohol hangover without any toxicity. Therefore, the content of acetaldehyde wasdecreasing and increasing through repeating 8 hours within 24 hours.

IntroductionNumber of kidney acute and chronic disease is increasing rapidly in the world and becoming the majorcause of death even population employment capacity is invalid. Statistical report of Mongolian Ministryof Health last 5 years statistic kidney disease is in the 3rd of non-contagious disease Synthetic andchemical medicines used for this sort of disease would have side effects in some cases. Plants, animalsand minerals biologically active substances, side effects need to produce new drugs, has attracted theattention of researches.GoalIdentifying pharmacology act of new granule medicine preparation.Material and Methods: The effects of the medicinal substances were investigated on “WISTAR” linesof white rat. Pathological model of nephritis was formed by injected the rats with kanamycin sulfate(Mondodoev.A.J, Lameza.S.B, Bartonov.E.A, 1988). The experimental animals were given any of thenew granular herbal medicine and compared to the rats given Nefromon. After treatment the creatinine,urea acid and MDA in the serum were determined. MDA is identified by an amount of concentration andmethod (Stalinaya. I.D. 1977).ResultCreatinine amount of disease model group of kidney illness created by kanamycin sulfate is comparedwith healthy group animals and 1.64 times, carbamide amount is 4.25 times, rest of the azote’s 2.73are increased and comparing the experiment group creatinine amount is 1.65 creatinine amount is 1.65decreased comparing with disease model group.ConclusionWhen compound ingredients preparation creates experiment animal kanamycin sulfate oxidantdominates, intensify the kidney cell active, decrease the carbamide and creatinine and decrease thekidney cell necrosis.

Introduction. Monos Group, Drug Research Institute is starting to investigate of Ellipin preparationfrom the mid-1990s, Ellipin has anti cancer activity in liver and several studies were investigated withscientists from Japan and China. Especially Hayashi K., Khurelbaatar L and Ambaga M were determinedanti-cancer action of the preparation and they were explained of mechanism of action, which apoptosisis seduced by influence of unsaturated fatty acids in tumor cells. However, changes of fatty acidscomposition at production stage were did not study yet. Therefore, we studied that composition of fattyacids in different term of production stage and compared of Ellipin dense substance.Materials and Methods. Samples of study were collected from production stage of “Ellipin” series130304, which was tacked in 48th hour, 120th hour of production. Each sample was dried at freezedryer “Labconco freezone12L” in Drug Research Institute. Total lipids of sample were extracted withchloroform: methanol (2: 1 v/v) according to Folch et al. Fatty acid methyl esters were analyzed usingAgilent Packard Gas Chromatograph (GC) (Model HP-6890 Agilent Packard) with mass-spectrumdetector (Model HP MSD 5973N) of Buryat State University, in Ulan-Ude.Results. Ellipin preparation is derived from bovine liver, and which is based on homogenization of bovineliver for isotonic. In this process, unsaturated fatty acids were extracted in organic solution. We studiedchanges which saturated and unsaturated fatty acids of bovine liver in process of homogenization andconsist of each fatty acid contents of end product. Results have shown that unsaturated fatty acidswere decreased by 0.4-44% till 120th hour of homogenization process. While, there were decreasedby 4-12% in the end product, although, ω-6 fatty acids were increased by 13.1-38.4%. Moreover, 25saturated fatty acids and 12 unsaturated fatty acids were detected in the Ellipin dense substance (endproduct). Hence, 67.5% of total fatty acid was saturated fatty acids, 32.5% was unsaturated fatty acidsin the Ellipin dense substance. Resent results and results of previous studies indicated that Ellipindense substance may contains saturated fatty acids on in average 50.34%, unsaturated fatty acids onin average 49,32%, respectively.Conclusion. Proportion of saturated and unsaturated fatty acids in Ellipin production was about 2:1.Saturated fatty acids and unsaturated fatty acids were found 25 and 12, respectively. Saturated fattyacids were gradually decreased and unsaturated fatty acids were slowly increased in production period,which from 48th hour of production-conveyer till end product. Moreover, content of ω-3-6-9 fatty acidswas consist 83,9-87,5% of total unsaturated fatty acid.

Purpose: In generally, T cell activation evaluates by CD4 +, CD8 +, B cell immunoglobulin's (IgG, IgM, IgA) in Azathioprine caused secondary immunodeficiency in serum. May it does not make clearly interpret for regulation of cell-mediated and humoral immune response using interaction T and B cells. In this review, we discuss the multifaceted roles of cytokines as enhancers and expression of B and T cell. Cell-mediated Immunity and Humoral Immunity Based on the type of immunodeficiency, the types of defects can vary. While some bacterial infections may be a key feature of B cell and T cell defects are feature of combined T and B cell immunodeficiency. Also other defects can be cause of phagocytes cell and complement deficiency. The present study provides evidence special cytokines which are involved to expression of T and B cells that CD40, IgG, IgM, FcyRII (CD32) need to be define with B cell deficiency, CD40, CD45RO define combined T and B cell deficiency and FcyRI (CD64), CD45RO define for phagocytes cell deficiency and CD4+, FcyRI (CD32), FcyRI (CD64) define for complement deficiency. In this review, we have done pharmacological study of immunosuppressant injection which is with Mongolian Astragalus Mongolicus Bge and determined CD28, CD40, CD64, and CD45 cytokines in the blood serum of mice. It will be clarifying the immune system interaction between cell-mediated immunity and humoral immune for T cells and B cell interactions.