IntroductionSpondylogenic lumbar pain is a common and disabling disorder in individuals and society. Therefore,this disorder to medical care is in second place in the cause of people with respiratory diseases later.Lumbar pain is a problem in our country and the world are common among the population in thedeveloped world, and is considered to be one of the reasons for limited movement. Electrotherapy,which is a noninvasive, non-pharmacologicalmethod involving transcutaneous electrical stimulation,is an additional alternative for low back pain management. The electrotherapymethods most used inclinical practice.GoalTo evaluate the efficacy of transcutaneous electrical nerve stimulation (TENS) with acupuncturecombination in patients with chronic lumbar spondylitis.Materials and MethodsThe study was done in department of Rehabilitation at “Bayanzurh” hospital, conducted based onthe material resources of the “New Medicine” university. Study design case control-clinical trial. Withspondylogenic chronic low back pain patients mean age was 39.24±8.27years. Symptom time was1.83±1.23year. Group A (n=30) used acupuncture and TENS, group B (n=30) used acupuncture,group C (n=30) control group. For pain Visual Analogue Scale (VAS), for quality-of-life Oswestrydisability index Questionnaire (ODI), were used before and after the treatment.This study protocol was approved by the ethics committee of “New medicine” university.ResultTo respondents age group: in the 20-29 age group 17(18.8%), in the 30-39 age group 23 (25.6%),in the 40-50 age group 50(55.6%). More than 40-45 years of age with spondylogenic chronic backpain. There were women 57(63.33%) and men 33 (33.67%), and shows that women had more sick.Total respondents assessment visual analog scale (VAS) of pain before treatment 3 group <0.334 nostatistical difference, after treatment was p <0.0001 is a statistical difference. The Oswestry disabilityindex (ODI) has been recommended as a back pain- specific measure of disability by researchers inthis field. The ODI is simple to read. Total respondents assessment Oswestry disability index (ODI) ofpatients before treatment 3 group p<0.066 no statistical difference, after treatment wasp<0.0001 is astatistical difference. The differences of pre- and post-treatment values of parameters were evaluatedfor each group. Significant improvements were detected for VAS, ODI group a post treatment.Conclusion. Transcutaneous electrical nerve stimulation (TENS) with in combination acupuncturetreatment is effective on pain, function and quality of life in patients with spondylogenic chronic lowback pain.

Introduction: There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals.Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset diabetes. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studies, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effect at relatively low cost. Materials and Methods: The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. The Prozorovski3 quick method for the determination of LD50 in the water (20%) and ethanol extractions (30%) of Antidiabetes-3 preparation (AD3). The tested animals were the white mice. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) 4 methodology of studying effects on immune system, we have Antidiabetes-3 preparation (AD3) were given to the 33 mice 2 times a day in 3ml/200gr dose, during 7 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre to screen RBC antibodies.Results: The method developed by V.B. Prozorovski for the calculation of average lethal number was used on 40 white mice (18-22g). Water extraction (10%) was per fused in the tail vein of the experience mouse and the lethal dose (LD50) was 88.9g/kg. These facts prove that the toxic effect of the AD is low. The water (10%) extractions of “Antidiabetes-3” (AD3) preparation were given to the mice 2 times a day in 3ml/200g dose, during 8 days. We have studying compared group “Salimon and Immunal mixture” (S&I) to the mice 2ml/200g dose, during 8 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and hemagglutinin to screen RBC antibodies (Table 1). Figure 1 demonstrates increase in mice’s spleen weight on the 5th day after stimulation of immunity with sheep’s RBC antigen. Spleen weight increase in AD3 group was 1.6 times higher compare to control group (AD3 group 0.16±0.08; control group 0.10±0.02; p<0, 05), and AD3 group was 1.0 times level compare to control group (AD3 group 0.16±0.08; S&I group 0.17±0.09; p<0, 05). In figure 2, the spleen index in control group was 1.24 times higher than in normal group (control group 0, 0047±0.001; normal group 0.0038±0.0004; p<0, 3), AD3 group’s index was 1.3 times higher compare to control group (AD3 group 0.0061±0.002, control group 0, 0047±0.001; p< 0.05), and 1.0 times lower compare to S&I group (AD3 group 0.0061±0.002; S&I group 0.0062±0.003; p< 0.05). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5 *106/ml. AD3 group’s spleen cell number was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutination titre of AD3 group was 1.13 times higher (AD3 group 54.86±19.95%; control group 50±8.83%, p<0,05) and this indicates that BV has immunity stimulating effect.Conclusions:1. Was defined the Antidiabet-3 preparation LD50, 88,9g/kg, its toxicity of classification (Sydorov K.K 1973) was little toxicity.2. Was defined to immunity stimulating effect the Antidiabet-3 preparation

Many plants have been used for the treatment of diabetes mellitus in traditional system of medicine and in other ancient systems of the world. Out of these only a few have been evaluated as per modern system of medicine. From many such plants only extracts have been prepared and their usefulness evaluated in experimental diabetes in animals. In some plants like extract Antidiabetes-3 (Cynarascolymus L,DasiphorafruticosaRydb. L,Tribulusterrestris) active hypoglycemic principles have been isolated and their mechanism of action studied. Most of them seem to act directly on in vitro assays to glucose uptake effects in normal and disiese human blood. Some have extra pancreatic effect also by acting directly on tissues like liver, muscle etc. and alter favourably the activities of the regulatory enzymes of glycolysis, gluconeogenesis and other pathways. Since the plant products have less side effects, they have the potential as good hypoglycemic drugs. They may also provide clues for the development of new and better oral drugs for diabetes. We have compared the in PBS of normal and disiese human blood, proves the glucose uptake effect of the Antidiabetes-3 preparation.

During many hundred years, our foregathers have used various medicinal plants. It is important that we should do research based on scientigic that to abstract biological activity plants that is unknown still now, to seen sharp and harmgul features, to assort with physical and chemical features etc. Historically in the traditional medicine Rhododendron Adamsi.Rуhd, Rhododendron nivale Hook.fwere used as the preparation against different types of irritations as they have certain effects to increase immune, to cure lung desease, to decrease blood pressure. Within the frame of our research we screened the impact of Rhododendron Adamsi.Rуhd, Rhododendron nivale Hook.fonto hepatocyte’s membrane pereoxidation.

Preparation of the water Extract from Antidiabet-31:10 was suspended in distilled water (100 mL) and allowed to stand at 4◦C. It was then filtered through several layers of muslin cloth and filtrate (water extract) was discarded Male Shinshila rabbits, weighing 1.5–2.7 kg, were rendered diabetic with an injection of 100 mg/kg alloxan monohydrate into a marginal ear vein. To reduce risk of nephrotoxicity from hyperuricemia, a 7 ml/kg body wt intravenous injection of 0.9% saline was given immediately after the injection ofalloxan. To counteract initial hypoglycemia, 3.5– 4.0 g glucose/kg body wt was given subcutaneously [27.5% (wt/vol) solution] 5– 6 h after the injection of alloxan. Diabetes was defined by a blood glucose concentration 16.9 mmol/l on 1 day. The total number of rabbits used was 28. Plasma was obtained by centrifugation of blood glucose, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol,triglycerides (TG), ‘’Humylazer 2000’’ chemistry analyzers automated (Human, Germany). As shown in Table 1, showed significant antihyperglycemic activity (p<0.05). Antidiabet-3 (AD3) and control failed to achieve euglycemia but caused a significant (p< 0.05) reduction in glucose levels compared to their initials values. The result of the experiment were confirmed that using by blood glucose in antidiabet-3 treatment groups taken for an observation at 3 dayfrom it was decreased to 22.20±2.5 (2.88%), post 7 day it was decreased 19.03±2.75 (14.3%), post 14 day it was 14.86±0.80 (33.06%), which it is showed may increase the blood glucose from diabetic rabbits.

Introduction: Diabetes mellitus is a chronic metabolic disorder resulting from insulin deficiency, characterized by hyperglycemia, altered metabolism of carbohydrates, protein and lipids, and an increased risk of vascular complication. Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset of diabetes. There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studied, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effects at relatively low cost. Materials and Methods: Preparation of the water extract from Antidiabet-3 1:10 was suspended in distilled water (100 ml) and allowed to stand at 4◦ C. It was then filtered through several layers of muslin cloth and filtrate (water extract) was discarded. Male and female Chinchilla rabbits, weighing 1.5–2.7 kg, were obtained from Biocombinat State Owned Enterprise, Ulaanbaatar, Mongolia. The total number of rabbits used was 30. They were housed at a temperature of 22 ± 20C with a schedule of 12 h light and 12 h dark cycle. They were acclimatized to laboratory conditions at least for 1 week beforecarrying out any experimental work. The experimental protocol for the present study was approved by Institutional Animal Ethical Committee (IAEC) of Health Sciences University of Mongolia. Experimental diabetes was induced in rabbits with alloxan monohydrate (100 mg/kg) (Sigma Chemicals, USA) injected intravenously to overnight fasted rabbits through their marginal ear vein. To reduce risk of nephrotoxicity from hyperuricemia, a 7 ml/kg body wt intravenous injection of 0.9% saline was given immediately after the injection of alloxan. To counteract initial hypoglycemia, 3.5-4.0 g glucose/kg body wt was given subcutaneously [27.5% (wt/vol) solution] 5-6 h after the injection of alloxan. Hyperglycemia of the rabbit with a permanent blood glucose concentration of >16.9 mmol/l was established 24 h after alloxan injection. For this study, a blood glucose level greater than 14 mmol/ liter (200mg/dl) was an indication of hyperglycemia. Aqueous crude extract of the Antidiabet-3 preparation was administration orally in dosage 0.5 ml/kg alloxan induced diabetic rabbits and fasting blood glucose monitored over a period of 14 days. Metformin in dosage of 7.4mg/kg was chosen as a comparative remedy. Results: The total number of rabbits used was 28. Diabetes was defined by a blood glucose concentration 16.9 mmol/l on 1 day. Plasma was obtained by centrifugation of blood glucose, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglycerides (TG), ‘’Humylazer 2000’’ chemistry analyzers automated (Human, Germany). The initial serum glucose concentration had an average value of 5.52±0.18 mmol/l in the serum. First days after injection of alloxan, the concentration had increased to 21.65±11.8 mmol/l. It reached its peak level of 30.47±2.55 mmol/l on the 3rd day. The level decreased, falling in all groups to 28.00±1.02mmol/l on day 14, control group. The differences between the control group and hyperglycaemic groups were statistically highly significant (p < 0.05). The result of the experiment were confirmed that using by blood glucose in antidiabet-3 treatment groups taken for an observation at 3 day from it was decreased to 22.20±2.5 (2.88%), post 7 day it was decreased 19.03±2.75 (14.3%), post 14 day it was 14.86±0.80 (33.06%), which it is showed may decrease the blood glucose from diabetic rabbits. The mean total activity of AST was increased 133.3 ± 18.1 u/l of that in the Antidiabet3 group. By day 14, this value had decreased by about 91.8* ± 4.01 u/l and Alloxaninduced diabetic rabbits administered with aqueous extract showed 31% decline in the activity of AST level on 1 and 14 day, respectively. The mean total activity of ferment ALT was increased 160.22 ±25.86 u/l of that in the AD3 group. By day 14, this value had decreased by about 91.8±4.01 u/l (42.7%). Alloxan-induced diabetic rabbits administered with aqueous extract showed 6.11%, 9.57%, 30.41%, and 24.45% decline in the activity of ferment level on 1, 3, 7, and 14 day, respectively. They can also improve the condition of diabetes as indicated by parameters like serum cholesterol, and serum triglyceride. It is now established that there is a gradual decrease in beta-cell function and mass that may occur in individuals at high risk of developing type II diabetes. To prevent the loss of beta-cell function and mass, beta-cell stabilization or regeneration must occur. The renewal of β-cells in diabetes has been studied in several animal models. For example epicatechin has been shown to act by β- cell regeneration. Conclusions: In conclusion, Antidiabet-3 preparation exhibited significant antihyperglycaemic activities in alloxan-induced diabetic rabbits. The establishment of diabetes mellitus in group I rabbits was observed after first week of alloxan administration by increased fasting blood glucose levels. Keen and NgTang (1982) reported that the minimum-defining characteristic feature to identify diabetes mellitus is chronic and substantiated elevation of circulating glucose concentration. Establishment of diabetes mellitus in rabbits in this study, induced by alloxan administration, might be attributable to specific irreversible toxic effects of alloxan on beta cells of pancreas (Dunn et al. 1943; Lukenes 1948).