The skeletal muscle materials sectioned from 20 cases of patients were histologically examined, who had all died of Kerschen disease. In addition to the simple atrophy and the hyaline degeneration which are generally regarded as lesions often found by autopsy of Kerschen disease, special types of muscle damage and reparation were noticed and described in the musculus gastrocnemius in 6 cases out of them, namely the myofibrillar dissociation of the muscle fibers and the connective tissue growth extending from the periphery to the internal part of the individual muscle fiber. The dissociated and even the necrotic muscle fibers could be distinctly limited within the area of the foci, while beyond the edge of the foci muscle fibers still remained almost unchanged. In the case of reparation the mesenchymatous elements in some region displayed their active action of ground substance production on the surface of the damaged fibers. But it was more noteworthy that a rare phenomenon happened: connective tissue growth got increased from outside into inside of the individual muscle fibers and at the same time it substituted for the position of the lost muscular substance. Thus in the cross section of a single complete muscle fiber muscular mass and connective tissue including its blood capillaries did stand side by side with an obvious contrast after the staining. This evidence naturally leads to the suggestion that a process of sarcolemmolysis may occur and allow the continuity of the connective tissue between the exterior and interior of a single muscle fiber. The disbanding of myofibrils within focus can also be explained by lysis of the sarcolemma and of its invagination, the transverse tubule system. In fact, it has not to be suggested, but was virtually revealed in electron microscopy, that sarcolemma of the damaged muscle fiber was lost. In place of the lost myofibrils a filamentous network was produced, which contained perhaps collagen stainable with aniline blue in Mallory's trichrome methode used for light microscopy. Theory of metaplasia about the reparation after the skeletal muscle lesions is however not supported by our observations. Besides, no regular correlation can be established between these special pathological changes of skeletal muscle and the Kerschen disease.

with the aim of how to explore the high effective proved recipes of chinese medicine, our research laboratory tried to propose that one may find out the corresponding diagnosis in term of western medicine as precisely as possible on the basis of the leading symptoms or leading signs which could be afforded by the case report on ideal cure with traditional medicine, and then correlate this diagnosis regularly with the high effective treatment. Therefore, not only the rich data about such diagnosis may be used to extend knowledge of the disease under study, but also a high effective recipe specific to the recorded disease can be obtained at first, then tested in practice. This paper describes two practical examples to illustrate the analytic Processes. One was to explore both acupuncture method and medical prescription for curing the rheumatoid arthritis complicated by subcutaneous nodules, and the other to identify the yam medical drink for treating the phosphaturia coming from the constitutional anomaly.

Objective To understand the biochemical characters of the intra-species of Semisulcospira and importance of paragonimiasis transmission. Methods The foot proteins of six species of Semisulcospira in Zhejiang Province were studied and compared by SDS-PAGE. Results 17-20 protein bands were found with molecular weight 27-162 kDa. Two strong bands,93 kDa and 52 kDa, were the same among the six species of Semisulcospira. The similitude coefficient was 0.74-0.85. Conclusion Two strong bands,93 kDa and 52 kDa, would be the protein markers of Semisulcospira, which may be related to the transmission of paragonimiasis.[

Objective To investigate the changes of melatonin and cellular immunological function in children with febrile seizures and its clinical significance. Methods 50 children, including 23 cases with complex febrile seizure (CFS) and 27 cases with simple febrile seizure (SFS) , and 25 cases with upper respiratory infections children selected as control group were enrolled in this study. Serum melato- nin was measured by enzyme-linked immunosorbent assay (ELISA) and cellular immunological function was measured by flow eytomcter. Results The levels of serum melatonin in the 3 groups of CFS, SFS, control were(14. 91±2. 61) ng/L, (20. 72±2. 54) ng/L, (23.93± 2. Ol) ng/L, respectively. The melatonin levels in CFS children were significantly decreased than that in control group and SFS children (P <0. O1). CD3 + ,CD4 +, the ratio of CD4 + /CD8 + and CD8 + in CFS group were significantly decreased than that in control group and SFS group (P <0.01). The ratio of CD4 +/CD8 + in SFS group was significantly decreased than that in control group (P <0.05), but CD3 + ,CD4 + and CD8 + had no statistics significance among these groups(P >0. 05). The serum rnelatonin level were positive related withdecreaseddegreeofCD3+,CD4+ andtberatioofCD4+ /CDS+ (r≥0. 472, P <0.05). Conclusion The disorder cfcellular immunological function was possible related with the loss of serum melatonin, and the loss of serum melatonin maybe one of the reasons for febrile seizures relapse and brain injured.

Objective To evaluate the efficacy and safety of valproic acid combined with all-trans retinoic acid(ATRA) in the treatment of myelodysplastic syndrome. Methods Twenty-two patients with MDS consisting of 4 RA, 1 RARS, 10 RCMD, 1 RCMD-RS, 3 RAEB-1 and 3 RAEB-2 were analyzed. The initial dose of valproic acid was 0.6 g/d, then increased to 1.2 g/d after one week; all-trans retinoic acid was administered at 30 mg/d every other week after taken VPA one week later. All patients maintained treatment for 3 months unless severe side effect was found or disease progression. Bone marrow examination was taken every 4 weeks. Results The response rate was 27.3 % (6 cases) according to International Working Group (IWG) criteria, no patient achieved completely remission(CR), 2 cases achieved partial remission(PR), 4 case achieved hematologic improvement(HI), 9 cases was stable and 7 cases was failure. Most of these cases had slight adverse events and could be tolerated. Conclusion Valproic acid combined with all-trans retinoic acid in the treatment of patients with myelodysplastic syndrome was efficient and side effect was tolerable.

0.05) and systolic velocity of regional myocardium, the difference between Ven and Vep, and mitral valve annulus have improved during the follow up study( P

This experiment was carried out to demonstrate substance P (SP) and VIP in mast cells in human skin by double immunoenzymatic staining, and to examine SP, VIP and serotonin in mast cells in mouse skin by dual immunoflourescence method. The results were as follows:1. There were three types of mast cells in human skin by double immunoenzymatic staining: i. e. SP immunoreactive mast cells, VIP immunoreactive mast cells and mast cells with coexistence of SP and VIP. In comparison with toluidine blue staining, 8.32％ of mast cells were VIP immunoreactive; 14.51％ were SP immnuoreactive; 7.71％ were SP and VIP coexistence cells.2. Similar to human skin, SP, VIP immunoreactive mast cells and mast cells with coexistence of SP and VIP were found in mouse skin by dual immunoflourescence. They were 12.7％, 7.9％, and 10.3% of she toluidine bleu positive mast cells, respectively, which acounted to about 24.6％ of the toluidine blue positive mast cells.The possible significance of these results were discussed.

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Objective:To systematically assess the value of 2 dimension color Doppler ultrasound(2D-CDUS) in diagnosis,surgical treatment and interventional therapy of carotid artery stenosis. Methods: Twenty seven carotid stenosis patients were examined before and after operation with 2D-CDUS. The morphology and hemodynamic of carotid artery were detected. Results: Pre-operative examination showed that there were 17 cases with stenosis degree being 50%-70%, 2 cases 71%-79%, 5 cases 80%-90%, 3 cases 90%-99%. Twelve cases were operated on with carotid endarterectomy(CEA), 15 cases with percutaneous transluminal angioplasty and stent(PTAS). After operation all the vessels with CEA were patent, colored well and blood velocity was close to normal. Residual intima was observed floating in the blood vessel in only one patient. In the patients treated by PTAS, the location and courser of the stents were normal,and the vessels were patent. Conclusion: 2D-CDUS is convenient to use and non-invasive, which can assess extracranial carotid stenosis accurately before operation, assess the curative effect and detect the complications after operation.

Objective:To assess the value of Transcranial Two Dimensional Color Doppler Ultrasound(TC-2D-CDUS) in diagnosis of carotid artery stenosis and in follow-up study after surgical treatment.Methods: Thirteen patients with carotid artery stenosis, who were excluded from vertebral or intracranial diseases, were detected before and after operation with TC-2D-CDUS. Results: TC-2D-CDUS indicated that 2 patients' image were not satisfactory, and the blood velocity of middle cerebral artery (MCA) in the same side of lesion was lowered to different extents in the other 11 patients. The waveform was blunted in 3 patients with their systolic peak disappeared, acceleration time prolonged, and the direction of the anterior cerebral artery(ACA) in the same side inverted.After operation,TC-2D-CDUS indicated that MCA blood velocity and the direction of ACA were normal. The blood distribution was symmetric in bilateral brains. Conclusion: TC-2D-CDUS can provide intracranial hemodynamic information. It can be used in the follow-up study after operation,helping to make accurate judgement and discover complications.