This paper reports four enhancing agents of interferon induction promoter in promoting induction of Namalwa cell interferon. In addition to the confirmation of the effects of the previously reported sodium butyrate and BrdU in raising interferon production by Namalwa cells, our study also shows that Acanthopanax senticosus polysaccharide, a classical Chinese herb medicine and sodium carboxyme- thyl starch possess the property of enhancing interferon production. When Namalwa cells are pre-treated with these two preparations, the mean titre of interferon is increased to 5 folds and 8-20 folds respectively. Possible operative mechanisms of this enhancement are discussed.

Objective:To study the expression of chemokine receptor CCR5 in follicular thyroid carcinoma and the serum level of CCR5 ligand,so as to assess the role of CCR5 in progression and metastasis of follicular thyroid carcinoma.Methods:Fifteen samples of follicular thyroid carcinoma,17 samples of follicular thyroid adenoma and 12 adjacent normal samples were analyzed immunohistochemically for CCR5 expression.The sera concentrations of CCL3,CCL4 and CCL5 were measured by ELISA in all patients.Results:CCR5 was positive in follicular thyroid carcinoma samples,with the positive rate being 73.33%,and was not detected in the follicular thyroid adenoma and the normal samples(P

Objective:To study the therapeutic effect of 2 artificially synthesized fusion peptides on scalded mice. Methods: Two new fusion peptides, M-T508 and M-T?1, were synthesized based on amino acid sequences selected according to the characteristics of scald (including 3 stages: inflammation, infection and repair by cell proliferation). The 2 peptides were applied for second degree scald burn in mice and the healing of the scald burn was observed. The expression ofⅠcollagen, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and cytokeratin (CK) was detected by immunohistochemical staining. Serum TNF-? concentration was determined by ELISA in the animals and the blood vessel permeability was analyzed by Evans blue staining. Results: The healing periods of scald burns in the 2 peptide groups were 1 week earlier than that in the control group. Immunohistochemical results showed that the expression of collagenⅠ, VEGF, PCNA and CK was all positive in the 2 peptide groups 10 days after scald and negative in the control group. Examination of blood vessel permeability and serum TNF-? concentration in the M-T?1 and M-T508 groups showed that the 2 fusion peptides had anti-inflammation and anti-infiltration effects in scalded mouse. Conclusion: The synthesized fusion peptide M-T?1 and M-T508 can promote the healing of scald burns in mice.

Objective:To investigate the role of CCR5 key residues as a co-recptor for the cellular entry of human immunodeficiency virus type Ⅰ(HIV-Ⅰ).Methods: Mutation of amino acids was introduced in different extracellular loops of CCR5 by site-directed mutagenesis technique,turning the non-polar amino acids into polar ones,the non-hydrophilic into hydrophilic,and the aromatic into non-aromatic.The mutants of CCR5 were expressed in BamHⅠ/XhoⅠ and were allowed to bind with gp120,and the binding activity of the mutants was compared with that of wild-type CCR5.Results: The coreceptor activity of CCR5 was reduced greatly when Cys in the first extracellular loop was replaced by Tyr and Pro in the third extracellular loop was replaced by Ser.There was no obvious change in the coreceptor activity of CCR5 when other replacements were introduced.Conclusion: HIV-Ⅰ virus needs receptor and co-receptor to achieve its cellular entry.CCR5 is a co-receptor and some of its extracellular loop amino acids are essential for gp120 recognition of HIV-Ⅰ.

Objective:To explore the anti-diabetogenic effect of antibody blocking portion of chemokine receptor-5 antibody(CCR5) molecule.Methods: The severe combined immunodeficient NOD(NOD.Scid) mice,injected i.p.with splenocytes from nonobese diabetic mouse,were randomly divided into 2 groups(8 of each group): anti-CCR5 Ab group and PBS group.The mice in anti-CCR5 Ab group were treated with a specific polyclonal antibody,targeting the first extracellular loop of CCR5,and PBS group was treated with PBS.Blood glucose levels were measured to observe the anti-diabetogenic effect of the antibody.Histological examination and ELISA analysis were also performed.Results: Within 70 d,all of the control mice developed diabetes while 5 of the 8 mice treated with anti-CCR5 Ab were diabetes-free.The difference of inflammation score between 2 groups was significant(P

Objective:To detect the expression of chemokine receptor CCR5 in human invasive ductal breast carcinoma and explore the role of CCR5 in breast cancer.Methods: Thirty samples of invasive ductal breast carcinoma and 10 samples of breast fibroadenoma were analyzed separately by immunohistochemical staining for CCR5 expression. MIP1?,MIP1? and RANTES concentrations were measured by ELISA in the sera of patients. Results: The expression of CCR5 was detected in invasive ductal breast carcinoma (the positive rate was 53.3%) and was not detected in breast fibroadenoma. RANTES concentrations in the serum of breast carcinoma patients increased significantly (P

Objective:To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library.Methods: The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph.D.-7 peptide library with phage display technique,and the positive clones were identified by ELISA.Results: After three rounds of biopanning,the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA.Sequencing result showed that the binding peptides had high affinity and specificity.Conclusion: A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library,which paves a way for the diagnosis and treatment of hepatitis B infection.

Objective:To establish adoptive transfer model mice of type 1 diabetes for investigating the pathogenesis of type 1 diabetes.Methods:16 NOD.Scid mice were randomly divided into two groups.One was injected i.p. with splenocytes which were obtained from a diabetic NOD mouse and the other was injected with splenocytes obtained from a normal one.The blood glucose levels and weight were detected daily.The mice were killed when they had significant clinic appearance or 10 weeks after cell injection,followed by pathologic studies and cytokine assays.Results:The incidence of adoption group was 8/8 whereas that of control group was 0/8.The pathology scores of 2 groups were 2.5?0.2,0 separately.The amounts of IL-2,IL-10,IFN-? were 60.7 pg/ml,15.5 pg/ml and 20.2 ng/ml respectively in adoption group,and those in control group were 2.4 pg/ml,17.5 pg/ml,3.2 ng/ml.Conclusion:Model mice of type 1 diabetes can be established in NOD.Scid mice during the 5-10 week after adoptive transfer of splenocytes of diabetic NOD mice.

The incidence of dysphagia after stroke is high.It may affect the rehabilitation of patients and increase mortality.The assessment is of great significance for early objective examination of dysphagia,in which the bedside screening tests are the most common assessment methods in the routine clinical work.This article reviews the advances in research on the commonly used bedside screening tests of dysphagia.

Objective:To screen for simulation peptide binding specifically to the first and the second extra-cellular domain of CC chemokine receptor 5(CCR5),and to observe their therapeutic effect on mice with experimental autoimmune encephalomyelitis(EAE).Methods:Phage display peptide library was used to screen for peptide sequence binding specifically to CCR5;ELISA was used to identify its binding activity and analyze its DNA sequence.The simulation peptide was synthesized and was injected into abdominal cavity of the EAE mice.Spinal cord tissues were obtained and the pathologic changes were studied by H-E staining in EAE control group and simulation peptide group.Results:Twenty phage clones were randomly chosen for identification and ELISA showed that there were eighteen clones had a strong binding activity with CCR5.The positive clones were sequenced and four peptides of high frequency were obtained:STFTTTL,TPIPQLL,SLPLPKP,and QTSSAAL.Mean clinical score of mice in the EAE model group was 3 and that of the simulation peptide group was 1.H-E staining found that the spinal cord tissues in EAE model group had great number of inflammatory cells and evident demyelination changes;the simulation peptide group had less inflammatory cells and no demyelination changes.The four short peptides had an effect of suppressing and delaying the development of EAE,with the average inhibition rate being 43%(P