Indoor air cleaner may improve indoor air quality which is closely related to the human health.The removal efficiency is one of important indicators to evaluate indoor air-cleaning devices.The indoor air cleaning techniques and its application were briefly introduced and the technique of evaluating indoor air cleaner for particle removal efficiency including choosing representative experimental particle,evaluation indicator and test rig were reviewed and discussed in the present paper.

From August 1989 to March 1994, 240 patients with esophageal carcinoma were studied. They were randomly divided into combined group (BFP chemotherapy plus radiotherapy, 120 cases), and radiotherapy alone group (120 cases). Both groups were treated by the same radiotherapy with dose of D T 50～70 Gy/5～7wk. The 1-,2-,3- and 4-year survival rates were 68.3%(82/120), 49.5%(47/95), 27.1%(19/70) and 15.6%(5/32) in BFP chemotherapy plus radiotherapy group and 44.1%(53/120), 28.4%(27/95), 22.9%(16/70), 15.6%(5/32) in radiotherapy alone group. The 1- and 2-year survival rates were obviously different in the two groups(P

Objective To know the hygienic administration status of central air conditioning system(CACS)and the Legionella contamination of cooling water in public places; and to identify the risk factors for legionella contamination associated with hygienic administration measures of CACS. Methods One hundred and forty-eight public places with CACS were recruited and the hygienic administration status was investigated by questionnaire survey in Sep. 2008. The culture methods were used to identify Legionella in cooling water. Results Positive rate of Legionella pneumophila in cooling water was 68.1%, 65.1% and 66.7% respectively in the condition of disinfection, preservative (detergent) and regular examination of the cooling water. The positive rate of Legionella pneumophila in cooling water was 92%, 87.7% and 84.7% respectively without disinfection, preservative,detergent and regular examination of the cooling water. Conclusion Disinfection, preservative,detergent and regular examination of the cooling water can reduce Legionella contamination risk.

Objective:To identify the influence of occupational stress on occurrence of hypertension.Methods:A prevalent cohort of 964 male workers employed for at least 3 years with different occupational stress were investigated with Occupational Stress Index, hypertension was diagnosed by WHO criteria of 1996. Results:The occupational stress was a risk factor for hypertension with RR=1.70 and in a dose-effect pattern after adjusted for other factors. In high exposure group, the adjusted incidence density was 12.47/per 1000 person-years with RR=2.99, in the medium exposure group, that was 8.81/per 1000 person-years with RR=2.11, that of low exposure group was 4.17/per 1000 person-years, the differences between different groups had statistic significance. When the length of exposure was more than 10 years, the adjusted incidence of hypertension increased significantly in high and medium exposure groups.Conclusions:Causality between occupational stress and hypertension exist, but it needs long exposure of at least 10 years for occurrence of hypertension.

Objective To analyze the molecular karyotypes of ANKA strain of Plasmodium berghei and demonstrate the size and number of chromosomes. Methods To isolate the genome DNA of P.berghei ANKA strain and analyze molecular karyotypes through CHEF-Ⅲ pulsed field gel electrophoresis (PFGE). Results The number of chromosomes was found to be 14, and their size ranged from 0\^6 Mb to 3 Mb. Chromosomes number 5 to 7 and 9 to 12 appeared co-migrated in the gel. Conclusion PFGE technique is useful for analyzing the molecular karyotypes and may be also useful for further study to locate the special gene on chromosomes and carry out the genetic characters and mechanism of drug resistance.

Objective To induce a line of Plasmodium berghei with resistance to artemisinin. Methods The major methods included blood transmission from passage to passage and progressive increase of drug pressure. Results The resistant lines were developed by different protocols: (A) The initial dosage of artemisinin was 126\^2 mg/kg which was increased by 60 mg/kg for the next passage and boosted by 126\^2 mg/kg for every other passage. As developed to passage 60 and 76, the resistant index was 18\^39∶1 and 14\^89∶1 respectively, then decreased gradually. For passage 108, the dosage was 8 862\^5 mg/kg, but the resistant index was only 10\^49∶1. (B) Using passage 66 from (A) as the source, a dosage of 4 000 mg/kg was given each week, the resistance of the passage 40 increased significantly with an index of 27\^5∶1. (C) Using passage 19 of (B) as the source, drug was administered at the dose of 2 000 mg/kg each week. The resistant index of passage 15 was 17\^41∶1. \{ Conclusion \} Line of P.berghei with medium level resistance to artemisinin was established.

Objective To investigate the pollution of parasitic protozoa in drinking water and wastewater in Shenzhen in or-der to present evidence to establish the national hygienic standard for parasitic protozoa in drinking water.Methods Water samples of resource water,fin ished wa ter from3water plants,and post-treated wastewater samples from3wastewater treatment plants in Shenzhen city were collected.Para sitic pro tozoa Giardia lamblia Stiles and Cryptosporidiumparvum were detected by following steps:filtering,washing,magnetic isolation and staining.Results Giardia lamblia Stiles and Cryptosporidiumparvum were not found in samples from raw water and finished water,while they were found in post-treated wastewater samples from2wastewater treatment plants.Conclusion The results indicated that the prob lem of Giardia lamblia Stiles and Cryptosporidi -umparvum pollu tion in the water supplying systems didnt exist at present,but Giardia lamblia Stiles and Cryptosporidi umparvum in the post-treated wastewater were the potential pollution source to surface water.

Objective To explore the differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neuron.Methods The mesenchymal stem cells were isolated from bone marrow. They were induced to differentiate into dopaminergic neurons by freated with bFGF, VitC and EGF at the third generation. Dopamine-associated protein and genes in the treated cells were examined by immumofluorescence and RT-PCR. Dopamine in the supernatant and endoplasm from culture system was determined by ELISA kit. Results The results showed that tyrosine hydroxylase, dopamine transporter and nerve neucleoprotein and Nestin,Nurr-1 genes were found. And the dopamine existed in the supernatant and cytoplasm from inducing culture system. Conclusion The rat bone marrow mesenchymal stem cells have the capacity of differenting into dopaminergic nurons.

Objective To study the effect of multi-disciplinary teamwork (MDT) nursing mode on perioperative care to breast cancer patients. Methods One hundred and twenty breast cancer patients undergoing surgical operations were evenly randomized into the observation and control groups by random digit number table. The control group was treated with routine nursing care and the observation group with MDT nursing mode. Result The rate of complications (like subcutaneous effusion, lymphatic edema and skin flap necrosis) in the observation group was significantly lower than that of the control group and the hospital stay was significantly shorter than that of the control group as well (all P<0.05). Conclusion The MDT nursing mode can reduce the rate of complications, shorten the hospital stay and relieve patient′s depression.

Objective To investigate the effect of tadpole extract(T871 3) on tumor cells and its mechanism. Methods We studied the effects of T871 3 on proliferation, differentiation and apoptosis of HL 60 cells by cytomorphological observation, cytochemistry and TUNEL method. We also examined gene expression during the induction of apoptosis and differentiation in tadpole extract treated HL 60 cells by in situ hybridization and intact cell mRNA dot blot techniques. Results 1 T871 3 was able to inhibite HL 60 cells proliferation. 2 T871 3 was able to induce HL 60 cells to differentiate along monocyte macrophage lineage at low concentration, and apoptosis at higher concentration. 3 The differentiation of HL 60 cells was accompanied by downregulations of c myc,c myb gene expression, The apoptosis of HL 60 cells was accompanied by downregulations of c\|myc bcl 2 gene expression, suggesting that these genes may be involved in the apoptosis and differentiation process. Conclusion Tadpole extract may have effects on HL 60 cells through changing the oncogene expression. [