Indoor air cleaner may improve indoor air quality which is closely related to the human health.The removal efficiency is one of important indicators to evaluate indoor air-cleaning devices.The indoor air cleaning techniques and its application were briefly introduced and the technique of evaluating indoor air cleaner for particle removal efficiency including choosing representative experimental particle,evaluation indicator and test rig were reviewed and discussed in the present paper.

Objective To study the part of Anti-thrombus and its property from Ground Beetle. Methods To dispart the Ground beetle into four segments which were shell, head, chest and abdomen, and adopt the method of frozen and melt to do the further test. Result Extracts of shell and chest were not active, extracts of head and abdomen were active. The activity of abdomen extracts was much better than that of the insect extracts. Its active component was glycoproteins. Conclusion The activity of abdomen extracts (Tf) is relatively high, and demonstrates wider value.

Objective: To study the role of occupational stress in CHD (coronary heart disease) Methods: 39 male attendants on train with CHD were identified through Rose questionnaie and were confirmed after one year Two control groups were collected according to the rate of 1:2 and matched by sex, age, job, year of working separately in railway system (n=78) and hotel service (n=78) All subjects completed OSI (occupational stress indicator) Logistic regression model was used to analyze the data Results: Exceptfor non-occupational stress, the relative risk of train attendant working was estimated as OR=2 18 with 95% confidence interval of 1 15～4 15, and the risk for CHD as a train attendant had dose-effect relation to occupational stress Compared with controls of railway system without CHD, the relative risk of stress was still significant (OR=4 43, 95% CI: 1 60～12 25) But the relative risk (OR=9 75, 95%CI:3 18～29 92) was much higher when comparing with controls of hotel service Conclusion: Occupational stress is one of the important risk factors for CHD in male attendants on train

Objective:To identify the influence of occupational stress on occurrence of hypertension.Methods:A prevalent cohort of 964 male workers employed for at least 3 years with different occupational stress were investigated with Occupational Stress Index, hypertension was diagnosed by WHO criteria of 1996. Results:The occupational stress was a risk factor for hypertension with RR=1.70 and in a dose-effect pattern after adjusted for other factors. In high exposure group, the adjusted incidence density was 12.47/per 1000 person-years with RR=2.99, in the medium exposure group, that was 8.81/per 1000 person-years with RR=2.11, that of low exposure group was 4.17/per 1000 person-years, the differences between different groups had statistic significance. When the length of exposure was more than 10 years, the adjusted incidence of hypertension increased significantly in high and medium exposure groups.Conclusions:Causality between occupational stress and hypertension exist, but it needs long exposure of at least 10 years for occurrence of hypertension.

Objective To know the hygienic administration status of central air conditioning system(CACS)and the Legionella contamination of cooling water in public places; and to identify the risk factors for legionella contamination associated with hygienic administration measures of CACS. Methods One hundred and forty-eight public places with CACS were recruited and the hygienic administration status was investigated by questionnaire survey in Sep. 2008. The culture methods were used to identify Legionella in cooling water. Results Positive rate of Legionella pneumophila in cooling water was 68.1%, 65.1% and 66.7% respectively in the condition of disinfection, preservative (detergent) and regular examination of the cooling water. The positive rate of Legionella pneumophila in cooling water was 92%, 87.7% and 84.7% respectively without disinfection, preservative,detergent and regular examination of the cooling water. Conclusion Disinfection, preservative,detergent and regular examination of the cooling water can reduce Legionella contamination risk.

[Objective] To observe the therapeutic effect of heat-clearing and diuresis-promoting herbal medicine for hyperuricemia induced by pyrazinamide. [Methods] Eighty-six patients with pyrazinamide-induced hyperuricemia were randomized into 2 groups: group A ( n = 43) received antiphthisic drugs (including isoniazid, rifampin, pyrazinamide and ethambutol) , allopurinol tablets and Modified Simiao Powder (one dose per day); group B ( n = 43) was treated with antiphthisic drugs and allopurinol tablets. Fourteen days constituted one treatment course and the two groups were treated for 2 courses. After treatment, the therapeutic effect was assessed and the changes of blood uric acid level were observed. [Results] In group A, 28 (65.12%) were cured, 12 (27.91%) effective, 3 (6.97%) ineffective and the total effective rate was 93.02%; in group B, 16 (37.21%) were cured, 17 (39.53%) effective, 10 (23.26%) ineffective and the total effective rate was 76.74% . The therapeutic effect in group A was better than that in group B (P

A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.

A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.

AIM:To investigate the effects of celecoxib on viability , apoptosis and autophagy in acute myeloid leukemia (AML) cell lines HL-60 and HL-60A.METHODS:The HL-60 cells and HL-60A cells were cultured with vari-ous concentrations (0, 20, 40, 60, 80 and 100μmol/L) of celecoxib.The inhibitory effect of celecoxib on the cell viabil-ity was evaluated by MTT assay .Apoptosis was analyzed by Annexin-V/PI staining.Apoptosis-related and autophagy-relat-ed proteins were determined by Western blot .RESULTS:IC50 of celecoxib were 49.4 μmol/L, 32.0 μmol/L and 25.1μmol/L for HL-60 cells treated with celecoxib for 24 h, 48 h and 72 h, respectively.For HL-60A cells, the corresponding IC50 were 69.1 μmol/L, 42.5 μmol/L and 29.6 μmol/L, respectively.The results of flow cytometry analysis showed the proportions of Annexin-Ⅴ+PI-, Annexin-Ⅴ+PI+and Annexin-Ⅴ-PI+cells were increased in the HL-60 cells, and those of Annexin-Ⅴ+PI-and Annexin-Ⅴ+PI+cells were increased in the HL-60A cells treated with celecoxib for 24 h. After treated with celecoxib , the induction of apoptosis was observed , the apoptosis-related proteins cleaved caspase-3 and cleaved PARP were upregulated , the autophagy-related proteins LC3 II and P62 were both increased , and mTOR, p-mTOR, 4-EBP and p-4-EBP were not changed , indicating that celecoxib inhibited autophagy in the AML cells without the mTOR pathway involvement .CONCLUSION:Celecoxib inhibits the viability of HL-60 cells and HL-60A cells in a time-and dose-dependent manner by its effects of inducing apoptosis and necrosis .Celecoxib inhibits mTOR-independent autoph-agy in AML cells, indicating a possible way of using celecoxib for enhancing the antitumor activity of therapeutic agents to induce cytoprotective autophagy in the AML cells .

Objective:To obtain highly purified recombinant human IGF-Ⅰ(rhIGF-Ⅰ) and identify it.Methods:rhIGF-Ⅰ Was purified through ion-exchange chromatography and gel filtration chromatography after the inclusion bodies of rhIGF-Ⅰ were extracted from Escherichia coli. The recombinant protein was characterized through molecular weight assay, Western-blot, and fluorescent chromatography. The renaturation and biological assay of rhIGF-Ⅰ were investigated. Results and Conclusions: The purity of rhIGF-Ⅰ was higher than 99%. The analysis of molecular weight, Western-blot, fluorescent chromatography and sequences of NH2-terminal 15 amino acids were same as those anticipated. 3-10 mg/ml was the concentration of renatured rhIGF-Ⅰ to support half-maximal stimulation of cell proliferation with BALB/c 3T3 cells.