1.The pharmacokinetic characteristics of amph otericin B liposomes injection compared with market injection in rabbits after intraveno us administration
Rongsheng ZHAO ; Baoxia YAN ; Xinpu HOU
Journal of Peking University(Health Sciences) 2001;33(3):243-246
Objective: To compare the pharmacokinetic c haracteristics of amphoterici n B liposomes injection (LAmB) with market amphotericin B injection (MAmB) in ra bbits by intravenous administration. Methods: LAmB and MAmB wer e intravenously ad ministrated to rabbits at a single dosage of 1 mg*kg -1. The AmB concent rations i n plasma samples were determined by HPLC, and the pharmacokinetic parameters wer e calculated by means of 3P97, and ANOVA were done by means of the Excel softwar e. Results: The concentration- time data of LAmB and MAmB after intravenous administration were best fitted acc ording to three compartment-model with a weight of 1/C and 1 respectively . After a singl e intravenous administra tion, C max of LAmB and MAmB were 8.4±2.1 and 2.4±0.6 mg*L -1, AUC were 22.5±6.8 and 9.0±1.9 mg*h*L -1, CL were 0.050±0 .024 and 0.114 ±0.022 L*h -1, V c were 0.13±0.04 and 0.46±0.18 L , respectively. Comparing with MAmB, all rabbits showed high plasma levels, large area under the curve values and low cl earance, small apparent volume of distribution of LAmB. Conclusion: There were several striking differences between the pharmacokinetics properties of LAmB and MAmB in jection in rabbits. All changes of the pharmacokinetics properties will be advan tageous to reduce toxicity and improve therapeutic effect.
2.Comparison of bioassay and HPLC for the determination of levofloxacin in plasma
Kaiying SI ; Jingli DUAN ; Baoxia YAN
Chinese Pharmaceutical Journal 1999;(1):43-
OBJECTIVE:The bioassay and HPLC methods were established and compared for the determination of levofloxacin(Lev) in human plasma.METHOD:In bioassay,medium Ⅱ and staphylococcus aureus were used.The HPLC method was chromatographed with a mobile phase of methanol-0.02mol/L potassium dihydrogen phosphate solution (3∶7),the sample was treated by adding 30% trichloroacetic acid to precipitate protein,and detected at 276mm.RESULTS:The average recovery of bioassay was within 95.90%~106.68%,the within-day and day-to-day precisions were below 6%.The average recovery of HPLC was within 97.24%~101.70%,the within day and day-to-day precisions were blow 5%.Lev tablets were given to 6 patients in two ways.Drug concentrations in plasma were determined by bioassay and HPLC assay,respectively,with no significant difference (P>0.05).CONCLUSION:The bioassay was simple,cheap,time saving,and reliable.The HPLC method wsa rapid,sensitive,accurate.The two methods may be selected to use in actual conditions.
3.Determination of tanshinoneⅡA in Rujie Xiaosan tablet
Yiheng YANG ; Xiaole ZHANG ; Shuqing WANG ; Baoxia YAN
Chinese Pharmaceutical Journal 2001;(1):56-57
OBJECTIVE To develope an HPLC assay for the determination of tanshinoneⅡA.METHOD C18ODS column was used.The mobile phase was consisted of MeOH-water(70∶30).The detection wavelength was at 269 nm.RESULT The linear regression equation was Y=9725X-2584,r=0.9997.The average recovery was 98.29% with RSD=1.86%.(n=5).CONCLUSION The method may be used for quality control.
4.Effects of different reperfusion strategies on clinical outcome of ST-segment elevation myocardial ;infarction patients
Pingshuan DONG ; Zhijuan LI ; Hongqiang DUAN ; Laijing DU ; Honglei WANG ; Ke WANG ; Peng YAN ; Xiyan SHANG ; Ximei FAN ; Ruiqing LIU ; Qiuling ZHAI ; Baoxia XIANG
Chinese Journal of Interventional Cardiology 2014;(3):172-175
Objective To evaluate the outcome of ST-segment elevation myocardial infarction (STEMI) patients received different reperfusion therapies. Methods The 238 consecutive STEMI patients were enrolled from February 2012 to December 2012. According to the current guideline of PCI and the choice of patients, the patients were divided into the groups of percutaneous coronary intervention (PCI), ifbrinolysis, and conservative medication. The major adverse cardiac events (MACE) was analyzed in a follow up of 6 months. Results (1) The enrolled patients included the 210 patients received PCI (88.2%), 14 patients received fibrinolysis (5.9%) and 14 patients received conservative medication (5.9%).The Median time of D2B was 110minutes.(2) The rate of late stent thrombosis was signiifcant higher in BMS than DES (n=2, 2.8%vs 0, P < 0.05) . (3) The PCI group had a signiifcantly higher incidence of stroke than the ifbrinolysis group and the conservative medication group (1.0%vs 0, P < 0.05;1.0%vs 0, P<0.05). (4) The PCI group had a signiifcantly higher incidence of bleeding compared to the thrombolysis group and the medication group (1.0% vs 0, P < 0.05; 1.0% vs 0%, P < 0.05). Conclusions The majority of STEMI patients received PCI;The D2B time, which was required<90 minutes in guideline of PCI, was found delayed in our study;Compared to ifbrinolysis and conservative medication, PCI showed better clinical outcomes of STEMI patients.
5.Preparation and studies on the quality standard of the liposomes carrier materials lecithin
Rongsheng ZHAO ; Baoxia YAN ; Xinpu HOU
Chinese Journal of Modern Applied Pharmacy 2001;18(1):39-41
OBJECTIVE:To prepare the liposomes carrier materials lecithin.METHODS:The lecithin was extracted and prepared from fresh yolks.The purity,the content,the percentage of oxidation and the quality standard were studied.RESULTS:The lecithin we prepared was chromatographically homogeneous phospholipid,with no indication of any other components.The content of lecithin was more than 98%,the percentage of oxidation was very low,and the results of studies on the quality standard were fit for the USP.CONCLUSION:The results showed that,the lecithin we prepared could be used as the liposomes carrier materials.
6.Significance of fecal occult blood test in colorectal cancer screening
Xiaoyue SUN ; Junjie GAO ; Gaiqin GAO ; Pengjin SUN ; Ke YAN ; Baoxia TIE ; Feng GAO
Cancer Research and Clinic 2019;31(6):422-424
Colorectal cancer is one of the most common malignant tumors of the digestive system. The incidence of colorectal cancer in China is increasing year by year. Screening for adenomas, precancerous lesions and early cancers can effectively reduce the incidence and mortality of colorectal cancer. Therefore, colorectal cancer screening is particularly important. At present, the colorectal cancer examination is mainly divided into fecal examination and colon structural examination. The fecal examination includes fecal occult blood test, fecal exfoliated cell test and fecal genetic analysis. The colon structural examination includes barium enema, colonoscopy and computed tomography colonography. Colonoscopy is considered as the gold standard for the diagnosis of colorectal cancer. It has the advantage of high accuracy, and can be used to find precancerous lesions and remove them at the same time. However, the screening should be greatly restricted because of its invasive examination, the need for intestinal preparation, obvious discomfort and the high cost of medical treatment. Comparatively, fecal occult blood test is simple, easy, non-invasive, saving manpower and material resources, and is suitable for large population screening. Some scholars in China have begun to pay attention to colorectal cancer screening, and fecal occult blood test is widely used in these screenings.
7.Data mining and analysis of oxaliplatin-related adverse events of nervous system
Baoxia FAN ; Yan KONG ; Ning LIU ; Ping YANG
China Pharmacy 2024;35(3):348-352
OBJECTIVE To provide reference for clinically safe drug use by mining oxaliplatin-related adverse drug events (ADE) of the nervous system. METHODS Oxaliplatin-related neurologic ADE data reported by the FDA adverse event reporting system (FAERS) between January 1st, 2004 and December 31st, 2022 were collected. The reporting odds ratio and proportional reporting ratio were used for data mining. The data were classified statistically by using systematic organ classification, high-level group term (HLGT) and preferred term (PT) in the MedDRA (version 26.0). RESULTS A total of 7 266 cases of oxaliplatin- related ADE, which were classified as various neurological, were retrieved, and 100 PT were identified. Of these, fifty-seven PT were unspecified adverse reaction signals in the manual. Among these reports, males (46.85%) were more than females (42.98%), the age of patients was 45-<75 years (65.22%), the number of reports was highest in Italy (16.32%), and the severe type was hospitalization or prolonged hospitalization (38.16%). The top 5 PT reports in the list of case number were peripheral neuropathy, paresthesia, neurotoxicity, loss of consciousness and dysarthria. The top 5 PT reports in the list of signal intensities were cold- induced paresthesia, neuromuscular rigidity, acute polyneuropathy, neuronal neuropathy, axonal and demyelinating polyneuropathy. A total of 13 HLGT were involved, with neurological diseases (not classified separately) having the highest number of signals (29). CONCLUSIONS When using oxaliplatin in clinical practice, it is not only necessary to monitor the high incidence of acute and chronic peripheral neuropathy, but also to pay attention to the patient’s consciousness state and neurological symptoms. We should pay attention to the rare types of adverse reactions, such as guillain-barre syndrome, Lhermitte sign, posterior reversible encephalopathy syndrome, and hyperammoniacal encephalopathy, so as to ensure the safety of medication.