This paper analyzed the effects of prosthesis selection and surgical technique in total knee arthroplasty (TKA) on patellofemoral joint biomechanics, patella movement track and prosthesis rotational alignment to explore the mechanics-related factors of knee joint function and prosthesis service life after TKA. Traditional anatomic landmark line lacks of accuracy due to various factors, so there is no uniform linear localization for tibial prosthesis. Femoral epicondylar axis functions as the reference axis of prosthesis rotational alignment in TKA, which can achieve ideal patellofemoral joint biomechanics, and reduce postoperative complications. 1/3 line of medial border to moderate medial border of the tibial tubercle is regarded as the ideal rotational interval o the tibial prosthesis, and introversion and extroversion degrees of the knee joint are determinant of linear localization. Knee symptoms and function are significantly improved after fixed and mobile-bearing arthroplasties. These two kinds of prosthesis do no significantly vary in knee joint, pain, function, and patella scores as well as radiography. However, the knee motions during maximum flexion in the mobile-bearing arthroplasty are smaller than in the fixed one. It has been proven by experimental studie that mobile-bearing arthroplasty shows wearing resistance and joint movement superior over the fixed one, but there is no agreemen in clinical studies.

During the development of science of acupuncture and moxibustion, the acupuncture monographs in Song Dynasty had been respected by scholars. Among those monographs, Tongren Shuxue Zhenjiu Tujing, which was compiled by WANG We-iyi in accordance with the emperor's command in Northern Song Dynasty, collected and collated the doctrine of the past, revised its error, designed and casted the bronze portrait as a model simulation, textually researched on meridian theory, and made a huge contribution for the development of acupuncture and standardization of acupuncture teaching and physician examination. In Southern Song Dynasty, WANG Zhi-zhong compiled the Zhenjiu Zisheng Jing. In this book those who met the traditional Chinese medicine and acupuncture theory and heired own clinical experiences were recorded together with practical guide on clinic. This book was instructive and meaningful to the clinical practice. This paper analyzed the lingual and cultural characters of these two reprehensive acupuncture monographs of Song Dynasty in a new viewpoint, and revealed the irreplaceable literature value, clinical value and cultural value of acupuncture monographs in Song Dynasty from their practicality, inheritance and innovativeness.

Objective To analyze the causes and the best operative methods of recurrent choanal polyp.Methods Clinical datas of 27 recurrent choanal polyp patients were analyzed.Results Of 27 recurrent choanal polyp patients,20 were recurred from antrum highmori,and 4 were recurred from lateral wall of mestus conchae.Furthermore,2 patients were under 8,14 patients were 8～14,11 patients were above 14.Ambiguity root of choanal polyp before operation,defective operation methods and not thoroughly treatment for operating field were the main causes of recurrence.Conclusion Finding the root of choanal polyp during operation and thoroughly treatment for operating field can effectively prevent the recurrence of choanal polyp.

Objective To analyze the diagnosis and treatment of cervical spinal cord injury without fracture or dislocation.Methods 38 patients were analyzed retrospectively.Results and Conclusion According to the manifestation of MRI and the symptoms,6 cases accepted conservation treatment,and the other 32 cases accepted surgery with different styles.All the patients recovered in some degrees.

Objective To explore the diagnosis and treatment of supinator syndrome.Methods 11 patients with supinator syndrome definitely diagnosed were treated with conservation treatment, 4 ineffective cases with conservation treatment were treated with operation. The following-up was performed for 6～12 months to evaluate the therapeutic effect.Results Among the total 7 patients received conservation treatment, 5 cases recovered completely, the other 2 cases recovered partially; among the total 4 patients who received operations, 3 cases recovered completely, and the other one recovered partially.Conclusion At present, supinator syndrome can be diagnosed definitely according to the clinical symptom, and the examination with electromyogram is beneficial to diagnose this disease, but it has not specificity. Most patients of supinator syndrome often get satisfactory effects after conservation treatment, and the ones who are failed can get the improvement from surgical treatment timely.

Objective Effects of melatonin and serotonin on ethanol induced ulceration in the rat stomach were investigated. Methods Serotonin was injected subcutaneously, then melatonin solution and finally ethanol was put into the ex vivo gastric lumen to prepare the experimental models. Glandular mucosal blood flow (GMBF) and the gastric mucosal injury were observed. Results Melatonin and serotonin administration did not induce observable gastric mucosal of damage in the ex vivo stomach, but the serotonin reduced glandular mucosal blood flow (GMBF) with dose dependence in this organ. Ethanol reduced GMBF and induced visible glandular mucosal damage. The latter effect was prevented by melatonin pretreatment. Serotonin pretreatment aggravated the gastric mucosal injury and GMBF changes induced by ethanol, but these effects were partially reversed by melatonin. Conclusion The findings indicate that the GMBF and gastric injury are related, the reduction in GMBF, however, may not be the sole factor responsible for ulceration. The antagonistic effects of melatonin on serotonin action on the stomach suggest that melatonin may play a modulator role for serotonin action on the gastrointestinal tract.

With the rapid development of Next-generation Sequencing(NGS)technologies, and its high throughput and low cost is applied widely in the field of life science, the increase in the depth of sequencing together decrease in the consumption of time and cost, makes a wide application of NGS in the research of microbiological research, ancient DNA study, clinical diagnosis, forensic science research, etc. The article discusses the second generation sequencing technology platform and its genetic markers in the forensic application. Included STR typing, SNP typing, HLA genotype prediction and the application in the degradation of the material.

Objective To disclose the inter-species differences among Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L using AFLP analysis. MethodsThe root, stem, foliage, flower, seed of Papaver somniferum L, foliage of Papaver rhoeas L and Cannabis sativa L were collected respectively. DNA was isolated with AxyPrep Kit, and double-digested by EcoR I and Mse I, then oligonucleotide adapters were ligated. After Pre-amplification, selective amplification was performed using 6 pairs of fluorescent primer. The DNA fragments were analyzed using a CEQ 8000 DNA Fragment Analyzer. Results27 to 46, 5 to 20 and 4 to 31 pieces of amplified products were detected from Papaver somniferum L, Cannabis sativa L and Papaver rhoeas L respectively, and significant difference was observed among these three groups of products. The identical species-specific peaks were identified from root, stem, leaf, flower and seed DNA from the same Papaver somniferum L. ConclusionThe diacritical results from Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L, as well as the identical results of different parts from the same plant demonstrated that AFLP analysis could be used possibly to determine the species origin of unknown plants samples.

OBJECTIVE:To establish a method for simultaneous determination of camphor and menthol in Compound diphen-hydramine liniment. METHODS:GC was performed on the column of Agilent 19095N-123 INNOWAX capillary column,column temperature was 120 ℃,injection volume temperature was 220 ℃,FID detector was used with the temperature of 260 ℃,carrier gas was nitrogen gas,flow of column was 6.0 ml/min,air was 450 ml/min,hydrogen gas was 40 ml/min,split ratio was 20∶1 and injection volume was 1 μl. RESULTS:The linear range of mass concentration was 0.612-6.12 mg/ml(r=0.999 9)for camphor and 0.593-5.93 mg/ml(r=0.999 9)for menthol;RSDs of precision,stability and reproducibility tests were lower than 2%;the recov-eries were respectively 98.0%-100.7%(RSD=1.0%,n=6)and 98.7%-100.7%(RSD=0.7%,n=6). CONCLUSIONS:The meth-od is simple,accurate and reproducible,and can be used for the contents determination of camphor and menthol in Compound di-phenhydramine liniment.

Objective Zinc sulfate has anti inflammatory action in many animal models, however, the effects of zinc in colitis remained uncertain. The present study was to evaluate the role of zinc sulfate in experimental colitis and probe into its underlying mechanisms. Methods Colitis was induced by administrating 2,4,6 trinitrobenzenesulphonic acid (TNB) rectally in Spragur Dawley female rats. Beginning at the first day of TNB colitis, the rats were treated with zinc sulfate enema once daily for 6 days. The rats were sacrificed at days 8. The effects of zinc sulfate were evaluated by examining mucosal lesion area, mucosal myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, mucosal prostaglandin E 2(PGE 2) and leukotriene B 4(LTB 4) levels. Results TNB induced severe colitis as evidenced by increased mucosal lesion area, mucosal MPO activity and PGE 2 and LTB 4 levels. Six days after the application of the zinc sulfate enema, the mucosal lesion area, MPO activity, PGE 2 and LTB 4 levels were all decreased significantly, except mucosal SOD activity that was remained unchanged after zinc treatments. Conclusions The data suggest that zinc sulfate enemas have an anti inflammatory action on experimental colitis through the mechanism other than increasing SOD activity.