Objective To observe the influence of chlorderazin on the change of L-arginine transport in human erythrocytes induced by oxidized low density lipoprotein. Methods Human erythrocytes were obtained from six healthy donors. The study included three groups: Gr1 (control)? Gr2 (incubated with OX-LDL) and Gr3 (incubated with OX-LDL and chlorderazin, a PLA 2 inhibitor). The 3H-L-arginine transport was measured.Results Compared with Gr1, the Vmax for the total transport of L-arginine in erythrocytes in Gr2 decreased by 33%, and Km value increased. Compared with Gr2, the Vmax for the total transport and the transport via system y+ of L-arginine in erythrocytes in Gr3 increased by 37% and 25% respectively, and Km values decreased for both. But the change of L-arginine transport by system y+L was not statistically significant.Conclusion OX-LDL could inhibit L-arginine transport in human erythrocytes, which could be reversed by chlorderazin, a nonspecific PLA 2 inhibitor.

Objective To explore clinical profile and significance of serum golgi protein 73 (GP73) in cirrhotic patients with chronic hepatitis B virus (HBV) infection .Methods A total of 109 cirrhotic patients with HBV infection were included ,in which 60 patients were compensated cirrhosis ,and 49 patients were decompensated cirrhosis .The receiver operating characteristic curve (ROC) analysis were used to evaluate the clinical significance of GP73 for diagnosing decompensated cirrhosis in cirrhotic popula-tion .Results Serum GP73 level in cirrhotic patients was correlated with alanine aminotransferase (ALT ) and HBV DNA ,but neg-atively correlated with albumin levels .Serum GP73 levels in patients with decompensated cirrhosis was (215 .9 ± 96 .56)ng/mL , which was significant higher than (113 .7 ± 68 .95)ng/mL in patients with compensated cirrhosis .The area under ROC was 0 .82 (95% CI :0 .74 ~ 0 .90 ,P< 0 .05) .The sensitivity and specificity of GP73 diagnosing decompensated cirrhosis in cirrhotic population with chronic HBV infection were 77 .55% and 75 .00% respectively ,when the cut-off was set at 140 .5 ng/mL .Conclusion Serum GP73 level is related with liver injury and HBV replication .GP73 might be an useful marker for diagnosing decompensated cirrhosis in cirrhotic population with chronic HBV infections .

ObjectiveTo analyze the distribution of complications of patients in different age in post-anesthesia care unit (PACU).Methods2679 patients in PACU were divided into three age group, and complications were observed and compared among each group.ResultsThere were statistics difference of recovery-time and incidence of complications among each group, except myopalmus.ConclusionIt is important to understand the difference of incidence of complications in different age, which may need different kind of nursing.

Objective To observe the X-ray appearance and clinical value of galactography.Methods 600 cases of galactorrhea confirmed by pathologly underwent galactography,among them,298 cases were blood type(49.8%),150 cases were serum type(25%),106 were rinsing type(17.7%) and others were 46 cases(7.7%).Results On galactography,the ductsl were divided into 3 groups: 345 cases were foliar type(59%),127 were ramate type(26.2%),87 were trunk type(14.8%).The main radiographic characteristics of lesions were mammary ductal ectasia in 578(96.3%),ductal distortion in 561(93.5%),irregular filling defect in ducts in 349(58.2%),and ductal damage in 39(6.5%).42 cases were breast carcinomas(7%),312 were intraductal papillary tumors(52%),129 were mammary ductal ectasia(21.5%),26 were mammary ductal mastitis(4.3%),49 were proliferous disease(8.2%),25 were mammary cyst(4.2%),17 were negative anamnesis(2.8%).Conclusion Galactography is beneficial in diagnosis of the pathogenesis of galactorrhea.

SUMMARY Left ventricular noncompaction(LVNC)is a rare congenital disorder of endomyocardial morphogenesis.Since the knowledge of aetiology and pathology is accumulating,the 2006 AHA cardiomyopathy classification sorts LVNC as one of the primary genetic cardiomyopathies.The clinical features of LVNC,however,is not as clear as its aetiology.We summarized the manifestation,hemodynamics,natural course,diagnosis,therapy and prognosis of LVNC by analyzing its clinical features of 2 cases and reviewing the latest related articles.

Objectives To observe the anesthetic effects of an improved anesthetic method on goat by comparing with the original method, and to provide theoretical and practical reference for anesthesia during the establishment of goat models.Methods A total of 72 goats were selected to make tibial fracture models, 36 were enrolled as group A and others as group B.The group A was injected with Sumianxin and diazepam.The group B was injected with Sumianxin, diazepam and pumped propofol by intravenous pump.The times of animal struggle, total dose of additional Sumianxin, death rate, and recovery time were evaluated.Results The death rate of group A was 16.67% and there was no death in the group B.The times of struggle, the total dose of additional Sumianxin and the recovery time of the group A were higher than group B, showing a significant difference (P<0.05).Conclusions The improved anesthetic method can decrease the times of goat struggle times, reduce the dose of anaesthetics, and lower the death rate.

BACKGROUND: The brittleness and low flexural strength of ceramic material hinder, to a certain extent, its application in prosthodontic dentistry. Zirconia is enhanced in its flexural strength and toughness by the transformation toughening mechanism, which makes up for the brittleness of the traditional all-ceramic material.OBJECTIVE: To preliminarily explore a new machinable zirconia ceramic material and investigate sintering properties of dental machinable zirconia/La-monazite diphase ceramics with nano-zirconia/La-monazite diphase ceramics. DESIGN: By adjusting the composition and ratio of raw materials, and by adopting different preparation and sintering method, this study was intended to measure the related parameters and to explore the best preparation and sintering method. SETTING: Department of Prosthodontics, College of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University; Lab of Advanced Inorganic Material Technology, School of Material Science and Engineering, Shanghai University. MATERIALS: There were 3 mol yttria-containing tetragonal zirconia polycrystals (3Y-TZP) (size≤50 nm, purity 99.99%, Yixing Xinxing Zirconia-products Co., Ltd.) and La-monazite (purity 99.99%, Baotou Rare-earth Phosphate Institution). METHODS: Experiments were performed at the Department of Prosthodontics, College of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University from January 2004 to December 2006. The pilot experiments found that the zirconia with less than 15% of lanthanum phosphate was high in strength but poor in machinability while more than 20% of lanthanum phosphate was decreased significantly in its strength. Therefore, 15%, 18% and 20% (volume percentage) of la-monazite was added to 3Y-TZP. The green bodies of the three groups were compacted by cold isostatic pressing (200 MPa) and were sintered in air atmosphere at different temperatures: 1 560 ℃, 1 580 ℃ and 1 600 ℃ to make the ceramic samples. MAIN OUTCOME MEASURES: The volume density, porous rate (Archimedes method) and three-point bending strength (EZ-100 universal testing machine) were tested of all the ceramic samples.RESULTS: ①With the increase of sintering temperature, zirconia/La-monazite ceramics with 15%, 18% and 20% lanthanum phosphate was increased in its bulk and density. The density was the highest for 1 600 ℃ and the respective density of the three groups were 5.77 g/cm3, 5.42 g/cm3 and 5.39 g/cm3. The porous rate decreased with the increasing temperature and was the lowest at 1 600 ℃ (0.88%, 1.21%, 1.49% respectively). There was no significant difference in volume and density at different temperatures (P > 0.05). ②The flexure strength of diphase ceramic with 18% and 20% lanthanum phosphate increased with the temperature increasing to 1 580 ℃. At 1 580 ℃, the flexural strength reached the highest level, respectively (772.22±43.43) MPa, (216.03±25.20) MPa and (157.21±9.79) MPa. When the temperature reaches 1 600 ℃, the strength was decreased. CONCLUSION: Zirconia/La-monazite diphase ceramics can be prepared by adopting cold isostatic pressing (200 MPa) and sintering at 1 580 ℃.

ObjectiveTo analyze the complication and outcoming of patients with deep hypothermic circulatory arrest (DHCA) in the operation of giant intracranial artery aneurysm.MethodsThe main complications and factors related to outcoming of six patients undergoing resection of giant intracranial basilar artery aneurysm were analyzed according to cerebral oximetry (rSO2), body temperature, electrocardiogram (ECG) during operation, and complications and outcoming after operation.ResultsUnder the condition of DHCA, complications such as ventricular fibrillation could not be avoided. Although circulatory arrest caused degrade of rSO_2, holo-brain oxygen deficiency caused by circulation arrest after operation was not happened in all six patients. Three patients were died after resection, and local brain injury occurred ins all six patients.ConclusionDHCA can induce multitude complication in the resection of giant intracranial artery aneurysm, local brain injury is the main factor of influencing the outcoming of patients and early rehabilitation is important to patients.