Objective To investigate the methods to repair the defection of finger tip.Methods During Feb.2004 to July 2008,in 98 cases,23 fingers were reconstructed with 2nd toe transfer which was reformed with digital flap or trilobed flap from 2nd toe,33 fingers were coverd with wrap around flap or half wrap around flap from great toe,or combined wrap around flap from great with pulp flap from 2nd toe,28fingers were repaired with fabular flap from great toe combined with tibial flap from 2nd flap,and 27 fingers were repaired with nail flap or enlarged nail flap from great toe. Results Vessel articulo took place in 5 cases,and all cases recovered with wonderful outward appearance Conclusion Microsurgical technic is an ideal method to repair the finger-tip defection.

Objective To explore the wound healing of semi-toe nail flap donor site after transplantation. Methods To repair small wounds,on 13 toes the first metatarsal dorsal flaps were applied,and on 7 toes the second toe digital flaps were applied.While,to repair large wounds,free flaps were applied,including foot inside flaps on 3 toes,peroneal artery perforator flaps on 5 toes,tarsal lateral artery flaps on 2 toes,and phil bowel artery inside branch flaps on 3 toes.Wounds remaining on semi-toe nail flaps of 7 toes were all covered with fibular island flaps of the first toes. Results Twenty cases were followed up,and the rang between 6 months to 5 years.The nail margin was exposed out of the lateral flaps in 2 cases.The first toes and the second toes appeared nearly normal in the other 18 cases.The flaps appeared smooth,flexible appearance with nice color and no ulceration.Seven cases applied with free flaps recovered protective sensibility with no complaint. Conclusion To cover wounds on semi-toe nail flaps of the first toes and the second toes with flaps is an ideal treatment method.The method of free flap transplantation is superior to vascular pedicle flap graft.

Objective To explore the correlation of TCM syndrome patterns of chronic pharyngitis(CP)with etiological factors.Methods A questionnaire investigation was carried out in 102 CP patients,of which 30 were differentiated as lung-kidney yin deficiency,30 as spleen-stomach deficiency,30 as phlegm coagulation and blood stasis,and 12 as spleen-kidney yang deficiency.The dietary hobbies,living and working environment,systemic diseases and living habits of the patients were recorded.Meanwhile,parameters of the pharyngeal microorganism and immunology were detected.Results The results of nonparametric test showed significant difference between the age and the mean disease course of the four syndrome patterns(P0.05). There existed significant difference between indulging in spicy food,indulging in cold and raw food,and staying up at night in the four syndromes (P0.05).The distribution of the four syndromes was different in the population with medical history of tonsillitis,dental caries,chronic cough,chronic gastritis and chronic colitis(P0.05).Conclusion The syndrome patterns of chronic pharyngitis are correlated with age,disease course,dietary habits of indulging in spicy food and cold-raw food,living habit of staying up at night,systemic disease or disease of organs around the throat,but are not correlated with sex,household registration,job,working and living environment,indulging in frying food,hobbies of smoking and drinking,living habits,emotional factors,throat swab culture results and immune parameters.

Objective: To study the 25 germplasm resources of Coix lacryma-jobi by the technique of SRAP molecular markers. Methods: Taking genome DNA in the leaves of C. lacryma-jobi extracted by modified CTAB method as template. Six ideal primer pairs were selected from 88 primer pairs by using the optimized SRAP-PCR reaction system and the amplification of 25 germplasm resources by SRAP-PCR were carried out. Results: The total 66 clear and repeatable polymorphic bands were obtained, and the polymorphic rate was up to 93%. The coefficient of genetic similarity among each species was calculated by using NTSYPC 2.1 software. The results indicated that the coefficient of genetic similarity of these materials ranged from 0.48 to 0.82. Conclusion: The 25 populations of C. lacryma-jobi could be clustered into four groups.

Objective To improve the outward appearanee in toe-to-hand transfer,a reshaped procedure of second toe was studied. Methods In reconstructing a finger with second toe transfer,a piece of flap from the fibular side of the second toe,or/and a H-shaped flap from the tibial of great toe were designed and transferred to the volar side in order to obliterate the constriction below the toe pulp. Results In 17 patients,22 fingers reconstructed with these procedure,ouly one rotation flap necrosis partly.The appearance of the newly reconstructed thumbs and fingers were improved,the pulp of reconstructed finger was similar to a natural finger, the narrow neck of second toe was well-stacked,and the interpbalangeal joint was straighten. Conclusion Reconstructed finger with this reshaped second toe is similar to a normal finger.

Objective To investigate the methods to cover the donor site of wrap around flap from toe. Methods In 34 cases, 8 thumbs reconstructed with wrap around flap from great toe, 8 thumbs and 14 fingers reconstructed with half nail flap from fibular side of second toe, and 4 fingers reconstructed with dorsal nail flap from second toe. The donor site were covered with pedicle flap (first metatarsal dorsal flap, second toe digital flap, neighbor flap from dorsal of second toe, and lateral tarsus flap) or free flap (medical plantar flap, fibular artery flap, and lateral tarsus flap). Results Twelve cases were followed up after 2 months, normal gait were found, 6 cases were followed up after 4 months, (4 cases for first metatarsal dorsal flap, one for fibular artery flap and one for internal volar flap). 2 cases recovered defend sensibility and showed a bit dark, the cases which reconstructed with medical plantar flap and second toe digital flap had nearly normal sensibility. The outward appearance of the donor site were chubbiness, flexible, and no complaint were found. Conclusion Covered the donor site of wrap around flap from toe with these flaps can avoid complications like pain and ulcerate, and have a satisfactory appearance.

Objective To explore the clinical, pathologic and immunohistochemical characteristics of gastrointestinal stromal tumor (GIST) and stromal tumor beyond the gastrointestinal tract. Methods Expression of CD117, CD34, SMA, Desmin and S-100 protein in 53GIST were detected using immunohistochemical S-P method, and then analyzed. Results Of 53 cases, 38 cases were detected as spindle type, 6 as epithelioid type and 9 as mixture type; 9 cases were benign,20 were borderline and 24 were malignant. Of the 9 benign cases, 8 originated in stomach, while only one originated in intestine and beyond the gastrointestinal tract. The rate of immunohistochemical expression was showed as follows: CD117 92.45% and CD34 77.36%. Conclusion GIST has its unique morphological characteristics. To detect CD117 and CD34 simultaneously may be helpful to the diagnosis. The malignant potential of stromal tumor in intestine and beyond the gastrointestinal tract is higher than that in stomach. The estimation of benignancy and malignancy depends on the site, the size and nuclei mitoses of the tumor.

Objective To explore the pathogenesis of substance P (SP) and epidermal growth factor receptor (EGFR) in early psoriasis vulgaris. Methods SP and EGFR were detected in normal skin tissues and psoriatic lesions by radioimmunoassay. Results Expression of SP was significantly enhanced in active and stable psoriasis than in recovery lesions and normal ones (P 0.05). Conclusion SP and EGFR may work together to play a key role in the early pathogenesis of psoriasis.

Objective To discuss the treatment of half hallux toenail flap transplantation for reconstruction of the distal segment finger defect. Methods Based on using the first plantar/dorsal metatarsal artery, or toe artery as pedicle to cut off partial toenail together with toe abdomen flap or partial toenail to repair 64 cases which include 69 fingers with distal segment complete and partial defect,the distal skin avulsion,skin tube in postoperative plastic,replantation failure and so on.Depending on the size of the donor foot wound use the first dorsal metatarsal artery,second toe artery,the perforating branch of peroneal artery or plantar metatarsal artery flap to cover the wound, and then observe the appearance of the finger and foot donor site after operation. Results The finger pulp was full and elastic,nail bed of the toe was well healing,nail groove which originated from the reconstructed nail fold was shallow,the appearance wos realistic,all feelings were recover,two-point discrimination was 4 to 5 millimeter,the donor toe was plump like the original one in appearance,patients were all satisfied with the reconstructed finger and the toe shape,after a follow-up from 3 month to 4 years. Conclusion Half hallux toenail flap transplantation is an effective way to repair the distal segment finger defect.

We expressed multi-epitope chimeric protein of CARDS toxin protein of Mycoplasma pneumonia (Mp) in prokaryotic cells,and purified and investigated its immunoreactivity.A recombinant multi-epitope chimeric gene including ten critical epitopes was connected by linker and cloned into prokaryotic expression vector pET-28a(+),and transformed into E.coli BL21(DE3) cells for expression under induction of IPTG.The antigenicity of expressed recombinant protein was identified with 6 × His monoclonal antibody and human positive serum by Western blot.The recombinant expression vector pET-CARDS was constructed and the about 30 kDa recombinant chimeric protein expressed in BL21(DE3) successfully.Western blot analysis showed that it can react respectively with 6 × His monoclonal antibodies and human positive serum.This study showed that the chimeric CARDS protein has an obvious immunoreactivity and a potential to be a new antigen for the diagnosis of Mp infection.