Objective To explore the methods of internal fixation for femoral neck fractures and evaluate the biomechanical characteristics of the interlocking expanding compressive screw (IECS)of Type HZS. Methods Based on the special biomechanical characteristics of the femoral neck fracture, the device of internal fixation was designed to consist of main screw, bifurcate screw, boost screw cap, washer, compressive screw cap, oblique interlocking screw and main screw cap. Its biomechanical performances were evaluated through comparison with those of the dynamic compressive screw (DCS), twin compressive screw (2CS) and three cannulated screws (3CS) respectively. Results The mechanical performances of the femoral neck fracture fixed with the interlocking expanding compressive screw were superior to those of the others in displacement ,axial and level stiffness, torsional strength and stiffness and terminal loading. Conclusion Interlocking expanding compressive screw can efficiently withstand the splitting, torsional, shearing, tensional and compressive forces in the fracture, and is simple, safe and easy to use. [

Objective : To evaluate the value of contrast-enhanced ultrasound quantitative analysis in the differential diagnosis of benign and malignant breast intraductal lesions. Methods ¡¤ A retrospective analysis was made on the characteristics of conventional ultrasound and the time-intensity curve (TIC) of contrast-enhanced ultrasound (CEUS) of 71 breast intraductal lesions in 63 patients, which were surgically resected and pathologically confirmed in Shanghai Tenth People’s Hospital from January 2016 to December 2018. The parameters of CEUS perfusion in the lesion area were obtained. Independent sample t test was used to analyze the differences of quantitative parameters between benign and ma-lignant lesions. The receiver operating characteristic (ROC) curve was used to evaluate the value of conventional ultrasound, quantitative analysis of CEUS and their combination for the differential diagnosis of benign and malignant intraductal lesions of breast. Results ¡¤ There were 42 benign lesions and 29 malignant lesions. Among the quantitative parameters of CEUS, time to peak (t=2.072, P=0.042), peak intensity (t=-2.629, P=0.011), rise slope rate (t=3.015, P=0.004) and the area under the curve (AUC) (t=3.308, P=0.001) were statistically significant. The sensitivity, specificity, accuracy, positive predictive value, negative predictive value and area under the ROC curve of conventional ultrasound were 31.03%, 90.48%, 66.20%, 69.20%, 65.50% and 0.608. Those of quantitative analysis of CEUS were 75.86%, 71.43%, 73.24%, 64.70%, 81.10% and 0.776. And those of their combination were 86.21%, 97.62%, 92.96%, 96.15%, 91.11% and 0.943. Conclusion ¡¤ Conventional ultrasound is of limited value in the diagnosis of breast intraductal lesions, and quantitative analysis of CEUS is of great significance in differentiating benign from malignant breast intraductal lesions. The combination of conventional ultrasound and quantitative analysis of CEUS can significantly im-prove the diagnostic value.

OBJECTIVETo explore the mechamisms of mitochondria-mediated pathway in apoptosis of platelets resulted from in immune induced bone marrow failure.

METHODSThirty C57BL/6 mice were randomly divided into 3 groups (10 mice in each group): normal group, model group, cyclosporine A(CsA) group. Mouse model of immune bone marrow failure were established. After mouse model was successfully established, the mice in normal group and model group were given saline orally, the mice in CsA group was treated with CsA orally. Blood routine examination of mice in each group was performed by automatic blood cell analyzer; the mitochondrial membrane potential(ΔΨm), cytochrome C(Cyt C), phosphatidylserine (PS), Cawere measured by flow cytometry; expression of BAX, BAK, caspase-3, caspase-8, caspase-9 was detected by using Western blot method, the changes of bone marrow platelet ultrastructure were observed under transmission electron microscope.

RESULTSCompared with normal group, the platelet count of model group decreased significantly, while the level of ΔΨm, caspase-3, caspase-8, caspase-9 significantly decreased, the level of Cyt C, PS, Ca, BAX, BAK increased significantly (P<0.05). Compared with the model group, the platelet count of CsA group increased obviously, while the level of ΔΨm, caspase-3, caspase-8, caspase-9 of CsA group increased significantly, the level of Cyt C, PS, Ca, BAX, BAK of CsA group decreased significantly (P<0.05). Electron microscopy showed that compared with the model group, platelet damage in CsA group were alleviated.

CONCLUSIONmitochondrial pathway plays an important role in the reduction of platelet resulted from immune bone marrow failure.

OBJECTIVETo determine the roles of mitochondrial apoptosis and energy metabolism in hepatocytes during the pathogenic process of acute renal failure (ALF) by assessing disease-related differential activities of several key mitochondrial enzymes, including citrate synthase (CS), carnitine palmitoyltransferase-1 (CPT-1) and cytochrome c oxidase (COX).

METHODSThirty-two male Sprague Dawley rats were given D-galactosamine followed by and lipopolysaccharide (LPS) to induce acute liver failure and sacrificed after 4 (4 h group), 8 (8 h group) 12 (12 h group) and 24 hours (24 h group) of treatment. Eight unmodeled rats served as controls. Effects related to apoptosis were evaluated by pathological analysis of hepatic tissues and TUNEL staining. Ultrastructural changes in mitochondria were assessed by electron microscopy. The activity and expression of CS, CPT-1 and COX were measured.

RESULTSHepatocyte apoptosis was present in the 4 h treatment group and was increased obviously in the 8 h treatment group. Hepatocyte necrosis was first observed in the 12 h treatment group and was significantly higher in the 24 h treatment group, with inflammatory cell invasion. Ultrastructural changes in mitochondria were present in the 4 h treatment group, and the 24 h treatment group showed mitochondria with completely destroyed outer membranes, which resulted in mitochondrial collapse. Activity and protein expression of CS, CPT-1 and COX were increased in the 4 h group (vs. controls), were at their peak in the 8 h group (CS:t =1.481, P less than 0.01; CPT-1:t =2.619, P less than 0.05; COX:t =1.014, P less than 0.01) and showed a decreasing trend in the 12 h group. In addition, the activities of CS, CPT-1 and COX were enhanced at the stage of hepatocyte apoptosis, suggesting that these enzymes were involved in the initiation and development of ALF.

CONCLUSIONEnergy metabolism plays an important role in hepatocyte apoptosis during ALF.

Animals ; Apoptosis ; Carnitine O-Palmitoyltransferase ; metabolism ; Citrate (si)-Synthase ; metabolism ; Disease Models, Animal ; Electron Transport Complex IV ; metabolism ; Hepatocytes ; cytology ; enzymology ; Liver Failure, Acute ; metabolism ; pathology ; Male ; Mitochondria ; ultrastructure ; Rats ; Rats, Sprague-Dawley

Atrial Fibrillation/surgery* ; Catheter Ablation ; Heart Conduction System/surgery* ; Humans ; Syncope, Vasovagal/surgery*

Objective:To observe the clinical efficacy of Kinesio Taping guided therapy on facial paralysis and salivation after stroke. Methods:From January to July, 2018, 30 patients with central facial palsy were randomly divided into control group (n = 15) and observation group (n = 15). The control group accepted ice stimulation, facial massage, facial muscle function training and low-frequency electrical stimulation, while the observation group accepted Kinesio Taping of "Y" or "O" shape alternately during massage and facial muscle function training, and kept taping for a day if possible. They were assessed with Teacher Drooling Scale (TDS), House-Brackmann (H-B) Scale and Facial Nerve Function Scale before and four weeks after treatment. Results:Both groups improved in the scores of TDS, H-B Scale and Facial Nerve Function Scale after treatment (Z > 2.460, t > 4.971, P < 0.05), and improved more in the observation group than in the control group (Z > 2.817, t > 4.964, P < 0.01). Conclusion:Kinesio Taping guided therapy is effective on central facial paralysis and salivation after stroke.