Aim: To evaluate the safety of glufosfamide in Beagle dogs. Methods: The safety of glufosfamide in Beagle dogs following single iv and multiple iv administration for 3 months was observed. Results: The minimum lethal dose of glufosfamide was 100 mg/kg. The maximum non-lethal dose of glufosfamide was 50 mg/kg. The approximate lethal dose of glufosfamide was 75-100 mg/kg. No serious adverse reactions was observed in Beagle dogs following multiple iv administration once a week for 3 months at the dose of 20 mg/kg, 40 mg/kg, and 60 mg/kg. However, organ injury was observed extensively in the high dose-group and mediumt-dose group. The low-dose group showed a light change in individual organs. Conclusion: Under the experimental conditions, 40 mg/kg and 60 mg/kg iv administration once a week for 3 months can lead to extensive organ injury in Beagle dogs, so the dose shouldn't be higher than 20 mg/kg.

Objective To observe the effect of aloe on intestine motility in the old costive mice and investigate the mechanism for aloe promoting an intestinal motility. Methods The content of aloin in aloe powder was determined by high performance liquid chromatography(HPLC).The old mice aged 15 months were randomly divided into 6 groups(10 in each group):blank control,positive control,constipation model,low-dose aloe,middle-dose aloe,high-dose aloe plus model.The mice of with equivalent volume of distilled water.On the eighth day,the mice except control group were given Compound Diphenoxylate to establish constipation model. With the black Indian ink as marker,the first time of black stool discharge,the character and weight of the stool,and the ink propulsion rate by intestines in mice were observed respectively.The serum level of nitric oxide(NO)was determined by spectrophotometry. Results The content of aloin in aloe powder was 0.266%.Compared with constipation model group,aloe groups in different dose decreased the first black stool time and increased stool grains and weight in 6 hours of constipated mice.The ink propulsion rates of intestines in the aloe groups were significantly higher than that of model group as well.The NO level in high-dose aloe group decreased more significantly compared with model group(P＜0.05),and there was a negative correlation between the serum NO level and propulsion rate of intestines(r=-0.346.P＜O.05). Conclusions Aloe could promote the mobility of intestine and ameliorate the constipation of mice,which might attribute to the decrease of the serum NO level.