The authors used high sensitive micellar enhanced fluorimetric method to monitor amiodarone blood-medical concentration. SLS severed as micellar reagent. Under experimental condition,we used 930 fluorophotommeter which made in China as the main analytic instrument,and obtained a series pharmacokinetic parameter ,eg. t_(1/2) (?) was 1.18 h, t_(1/2)(?)was 40.75 h, K_(21) was 0.278 l/h,K_(10) was 0.036 l/h,K_(12) was 0.291 l/h. The linear range of this method was 2 ? 10_(-9)～8 ? 10_(-6) mol/L,determined limit was 1.3 ? 10~(-9)mol/L, average recovery was 99.93%.

Objective To evaluate and compare the therapeutic effects of the open prostatectomy(OP)?transurethral electrovaporization ablation prostatectomy (TUEVAP) and interstitial laser coagulation(ILC) for the treatments of symptomatic BPH. Methods 282 symptomatic BPH patients randomly underwent OP (n=98)?TUEVAP(n=91) or ILC(n=93) respectively and the therapeutic effects were evaluated. Results There are significant improvements in symptoms and physical signs in each group after the procedure.The average catheterization time following OP?TUEVAP and ILC was 185 h?76 h and 362 h,the immediate morbidity rate being 28.9%?16.7%? 14.8 % and the retreatment rate 1.0%?6.6% and 16.1% respectively. OP group showed the longest average operative time and the most blood loss both of which were the least in ILC group. Conclusions OP is the most effective of the three procedures, but the trauma to the patient is the most.The therapeutic effect of TUEVAP is similar to OP's,with less injure.ILC is the easiest and safest procedure with the least side effects.

Objective To clone cDNA of trichosanthin (TCS) and purify TCS, and to study its influence on apoptosis and growth inhibition of colorectal carcinoma LoVo cells in vitro. Methods MTT assay was adopted to measure the growth inhibition ratio of LoVo cells treated with TCS, and apoptosis was assayed by agarose gel eletrophoresis. Results The results showed that the higher concentration of TCS and the longer testing time, the stronger growth inhibition of LoVo cells. DNA agarose gel eletrophoresis showed a gradient, which confirmed that TCS could induce the apoptosis of LoVo cells. Conclusions TCS can inhibit the growth of LoVo cells in vitro and induce its apoptosis. Our study provides evidence for the application of TCS in clinical treatment of human colorectal carcinoma.

ObjectiveTo explore the diagnosis and the surgical operation of concealed penis, and raise the therapeutic efficacy.Methods16 cases of concealed penis were reviewed retrospectively.ResultsAll 16 cases were treated by Devine surgery and got the satisfactory effect, the penis recovered to its good external appearance. All 16 cases had been followed up for four months to two years. The revelation of penis and its external appearance were all satisfied. No complications such as hydrophallus, skin ischemia, haematoma and erection ache occurred.ConclusionThe real concealed penis should be strictly distinguished from buried penis, microphallus and redundant prepuce, and suitable operation pattern can obtain satisfactory effect.

Objective To investigate the voicing acoustic characteristics of sulcus vocalis patients with mini-mum glottal area(MGA) .Methods 60 normal subjects (30 male ,30 female) ,and 62 subjects with sulcus vocalis (33 male and 29 female)were recruited .They all had stroboscopic laryngoscopy ,and had MGA measured when say-ing /i:/using software supporting KIPS .The acoustic parameters consisted of vocal cord fundamental frequency (F0) ,jitter and shimmer ,normalized noise energy ( NNE) ,harmony to noise ratio (HNR) and signal noise ratio (SNR) ,maximum phonation time(MPT) and respectively make correlation analysis with voice MGA .Results The voicing MGA of male patients with sulcus vocalis was 434 .74 ± 112 .83 ,larger than the males with normal vocalis 298 .25 ± 93 .63 .This was statistically significant (P<0 .05) .The voicing MGA of females with sulcus vocalis was (484 .75 ± 143 .91) ,significantly larger than those of females with normal vocalis (293 .43 ± 93 .73) and the differ-ence was statistically significant (P<0 .05) .The voicing MGA on both males and females with sulcus vocalis was noticeably relation to the F0 (r=0 .972 ,P<0 .05) and (r=0 .928 ,P<0 .05) ,Jitter (r=0 .978 ,P<0 .05) and (r=0 .910 ,P<0 .910) and Shimmer (r=0 .973 ,P<0 .05) and (r=0 .921 ,P<0 .05) ,normalized noise energy(r=0 .883 ,P<0 .05) and (r=0 .960 ,P<0 .05) ;and negative relation to the signal noise ratio (r= -0 .947 ,P<0 .05) and (r = -0 .957 ,P<0 .05) ,signal noise ratio (r= -0 .959 ,P<0 .05) and (r= -0 .944 ,P<0 .05) ,max-imum phonation time (r= -0 .891 ,P<0 .05) and (r= -0 .936 ,P<0 .05) .Conclusion Patients with pathological sulcus vocalis have glottal areas larger than normal .Acoustic analysis of the voices of the sulcus vocalis can be used as an objective laboratory examination .

Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

OBJECTIVETo investigate the diagnosis and treatment of renal trauma.

METHODSRetrospective analysis of 298 patients with renal trauma was carried out. Among them, 272 (91.3%) had blunt renal injuries and 26 (8.7%) had penetrating injuries; 123 (41.3%) had multiple complicated intraabdominal injuries and 56 (18.8%) had concomitant shock. Normal-dose-IVU examination was used in 39 patients and double-dose-IVU in 44 patients, ultrasonography in 109 patients, and CT in 45 patients. Conservative and supportive therapy was done in 193 patients (64.8%) and operation in 105 patients (35.2%).

RESULTSThe positive rate was 48.7% by the normal-dose-I VU examination and 90.9% by double-dose-IVU, 78.8% by ultrasonography, and 95.6% by CT. One hundred and eighty-three patients were cured by conservative therapy and 101 by operation. Fourteen patients died.

CONCLUSIONSB-ultrasound can be conveniently used for primary assessment of renal injuries, while CT shows rapid, accurate and proper condition of a renal trauma patient. The treatment depends on the severity of the injury. The conservative therapy is employed in most cases which present slight or moderate injury and no evident massive bleeding. Severe injury requires surgical exploration. The operative approach is by using a transabdominal incision, which makes it relatively easy to explore intraabdominal organs and control the injured kidney. It is also very important to control shock and prevent other severe complications in the early stage of the treatment.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Humans ; Kidney ; injuries ; Male ; Middle Aged ; Retrospective Studies ; Tomography, X-Ray Computed ; Treatment Outcome ; Ultrasonography ; Wounds and Injuries ; diagnosis ; diagnostic imaging ; therapy

Objective:To investigate the efficacy and safety of combined catheter-directed thrombolysis and anticoagulation for acute pulmonary embolism combined with lower limb deep vein thrombosis.Methods:A retrospective analysis was conducted using a case-control study approach on the clinical data of 58 patients with acute pulmonary embolism who received treatment at the Inner Mongolia Autonomous Region People's Hospital from August 2021 to August 2023. These patients were categorized into two groups based on whether they had lower-limb deep vein thrombosis or not. The group with pulmonary embolism alone ( n = 14) underwent targeted catheter thrombolysis in combination with anticoagulation treatment using low molecular-weight heparin. The group with both pulmonary embolism and deep vein thrombosis in the lower limbs (combined group, n = 44) received inferior vena cava filter placement, along with targeted catheter thrombolysis and anticoagulation treatment with low-molecular weight heparin. The improvement in pulmonary circulation was observed before and after the surgical interventions in both groups. A comparison was made regarding the improvement in indicators including arterial oxygen pressure, fingertip oxygen saturation, the proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area, and respiratory rate, both before and after pulmonary artery catheterization and thrombolysis in both groups. Results:The surgery was successful for all patients. The symptom of shortness of breath was obviously relieved after surgery. After surgery, in the pulmonary embolism alone group, the respiratory rate was (22.75 ± 4.38) beats/min, the mean pulmonary artery pressure was (32.53 ± 3.63) mmHg (1 mmHg = 0.133 kPa), the arterial oxygen partial pressure was (81.46 ± 7.24) mmHg, and the fingertip oxygen saturation was (90.53 ± 4.57)%, respectively. In the combined group, the respiratory rate, mean pulmonary artery pressure, arterial oxygen partial pressure, and fingertip oxygen saturation were (23.35 ± 5.8) beats/min, (31.34 ± 4.53) mmHg, (82.34 ± 4.62) mmHg, and (92.57 ± 3.45)%, respectively. After surgery, the respiratory rate, pulmonary artery pressure, arterial oxygen partial pressure, and fingertip oxygen saturation in each group were significantly higher compared with the corresponding levels before surgery ( t = 6.33, 12.12, 17.32, 8.13, 6.86, 13.02, 15.52, 7.20, all P < 0.001). The proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area in the group with pulmonary embolism alone was (19.56 ± 7.53)%, while in the combined group, it was (20.63 ± 4.83)%. After surgery, the proportion of pulmonary artery trunk thrombosis area to pulmonary artery trunk area in each group decreased compared with the corresponding value before surgery ( t = 19.36, 18.23, both P < 0.001). Conclusion:The surgical method combining catheter-directed thrombolysis with inferior vena cava filter placement is effective and safe for the treatment of acute pulmonary embolism combined with deep vein thrombosis in the lower limbs.