The spirit of Betheune is the majorpant of the regloation of medical ethics in China. Based on local facts, this article talks of the means and experience of enforcing the hospital staff with the spirit of betheune to seta good profession image.

Objective To explore the effect of Baogan Jiedu Granule on liver cell ultrastructure of model mice with acute liver injury induced by tetracycline.Methods Totally 60 mice were randomly divided into tetracycline model group,large-dosage,medium-dosage and small-dosage groups of Baogan Jiedu Granule,Ganlixin group and control group.Each group was respectively perfused with corresponding drug for 5 days and tetracycline was perfused once to make the model.The tissues were observed by electron microscope,and the liver cell ultrastructure was measured and analyzed by three-dimensional metrology.Results The amount of mitochondria Vv,heterochromatin Vv,mitochondria ?m,endoplasmic reticulum ?m and Golgi's apparatus ?m all obviously decreased in model group,while that of euchromatin Vv and lipid droplet Vv increased.Baogan Jiedu Granule could remarkably increase the amount of indexes referred above,and decrease the amount of euchromatin Vv and lipid droplet Vv.Conclusion Baogan Jiedu Granule can significantly ameliorate the pathologic change of liver cell ultrastructure,thus having a good hepatoprotective effect.

Objective To explore the effects of Baogan Jiedu Granule on lipid peroxidation of free radicals in acute liver injury mice induced by tripterygium glycosides.Methods Totally 60 mice were randomly divided into tripterygium glycosides model group,large-dose,medium-dose and small-dose groups of Baogan Jiedu Granule,Ganlixin group and control group.Each group was respectively perfused with the corresponding drug for 5 days and tripterygium glycosides were perfused once to make the model.The serum levels of ALT,AST,SOD,MDA and GSH-Px were measured.Results The serum levels of SOD and GSH-Px in model group decreased and MDA rose obviously.Compared with model group,Baogan Jiedu Granule of all doses could effectively decrease ALT,AST and MDA level and obviously improve GSH-Px level in each group.In addition,in the large-dose group SOD level was markedly improved(P

Objective To investigate the changes of platelet aggregation rate and platelet surface CD 41+CD62P+ expression af-ter clopidogrel discontinuation in the patients with percutaneous coronary interventions (PCI)) .Methods The platelet aggregation rates and platelet surface CD41+CD62P+ in 52 PCI patients with oral clopidogrel for near 12 months and discontinuation soon were measured before clopidogrel therapy(T0 ) ,in 1 week after clopidogrel therapy(T1 ) ,1 week before clopidogrel discontinuation(T2 ) , 1 week(T3 ) and 1 month after clopidogrel discontinuation (T4 ) .Results Compared with T0 ,the platelet aggregation rate and the expression of platelet CD41+CD62P+ at T1 were significantly decreased ,the difference showing statistical significance (P<0 .05) , which at T2 maintained the lower levels ;which at T3 were increased ,which at T4 were recovered to those at T0 .The platelet aggre-gation rates at various time points were (44 .20 ± 18 .36)% ,(25 .38 ± 12 .10)% ,(23 .74 ± 8 .15)% ,(51 .79 ± 10 .55)% and(45 .97 ± 16 .42)% respectively ,and the positive rates of CD41+ CD62P+ were(12 .96 ± 11 .48)% ,(3 .93 ± 3 .33)% ,(4 .72 ± 3 .14)% , (13 .90 ± 10 .38)% and(10 .84 ± 8 .13)% ,respectively .Conclusion In the patients treated with 12-month clopidogrel after PCI ,the platelet aggregation rate and the CD41+CD62P+ positive rate are mildly increased at 1 week after clopidogrel discontinuation and gradually returned to the level before discontinuation at 1 month after clopidogrel discontinuation .

Background Mitochondria are the primary sites for ROS production within cells,Tempol(4-Hydroxy 2,2,6,6,tetramethyl piperidine)is a classic compounds targeting ROS scavengers in mitochondria.Objective To investigate the effects of Tempol on aortic function and remodeling in renovascular hypertensive rats.Methods The 2 kindey 1 clip hypertensive model was established in 24 male Wista rats and randomized to untreated hypertensive rats(n=6) or treated with Tempol(1 mmol/L) in drinking water(n=6) for 8 weeks.BP blood plasma angiotensinⅡ(AngⅡ),nitric oxide(NO),8-iso-PGF_(2?) level were determined.Isometric tension change of aortic rings were recorded;RT-PCR were used to measure the expression of NADPH p22 phox mRNA of aorta.Results Hypertensive rats had highter BP,AngⅡ,8-iso-PGF_(2?),media wall,media wall/lumen(W/L)(P

Objective To study the preparation of Fructus perrillae oil nanoemulsion and its quality evaluation. Methods The formulations of Fructus Perrillae oil nanoemulsion were optimized by drawing the pseudoternary phase diagrams and investigating the form, viscosity, particle size distribution, stability, conductance, and refractiratio. Results The optimal formulation was composed of wheat oil and cremophor RH40, electron microscopy presented Fructus Perrillae oil nanoemulsion as small spherical drops, viscosity was (5.49?0.02)/mm2?s-1, conductance was 316/ms-1, refractiratio was (1.124?0.002 50), and mean diameter of particle was (50.3?1.1) nm.The average recovery determined by GC was 98.9%, its mean RSD was 0.88%. Conclusion The solubility and stability of Fructus Perrillae oil are promoted by nanoemulsion with better produncibility. It is a favorable medical matrix.

Objective To investigate the present situation of diabetic foot knowledge among nurses in Changzhi of Shanxi Province. Methods Adopting convenience sampling,100 nurses were recruited from 3 secondary hospital in Changzhi of Shanxi Province and evaluated by the questionnaire making by author. Results The average level of DF among nurses was unsatisfied. There are significant deference between who experiencing continuing education and not. Conclusions The present situation of diabetic foot knowledge among nurses in rural areas in China which should arouse more attention from management of nurse in rural area. Continuing education and professional training education should be carried out for nurses in rural area who also should develop their ability to read article,and Cognition of prevention and health education.

According to the specialty characteristics of medical college and university and the reality of teaching on the analytic chemistry experiment,this article explored a new thought on teaching of analytic chemistry experiment for majors of medicine and pharmaceutics.By optimizing the course teaching system,enhancing the requirement of experience teaching,renewing the teaching contents and integrating basal experiment with integrated and designed experiment,we obtained good teaching effect.

Objective:To explore the expression of IL-21 and the relationship with adenine nucleotide translocator antibody, ( ANT antibody ) in different courses of viral myocarditis ( VMC ) mice, and to explore the role and significance of IL-21 in VMC.Methods:To induced VMC mice model, BALB/c male mice were peritoneally injected ( IP ) with Coxsackievirus B3, mice peritoneally injected with PBS were taken as the controls.On 0,1,2,3,4 and 6 w after IP,the level of IL-21 mRNA in mice myocardic tissue was determined by real-time RT-PCR.The expression of IL-21 protein and ANT antibody in serum was evaluated by EILSA.Results:Comparing with the controls,the expression of IL-21 mRNA and protein increased from 1st week after IP,reaching the peak on the 2nd week,and maintaining a higher trend until 6th week.All the results at each time except that of 0 week were higher than those of controls(P<0.05).The levels of ANT antibody were also increased from 1st to 6th week,reaching the peak on the 2nd week all the results at each time except that of 0 week were higher than those of controls(P<0.05).Pearson analysis showed that there was rela-tionship between IL-21 and ANT antibody( r=0.88,P<0.01).Conclusion:The datas show that local significantly increased levels of IL-21 in myocardium and serum, and have positive relationship with the level of adenine nucleotide translocator antibody, which indicates that IL-21 may play a role in the pathogenesis of mice virus myocarditis.

Objective To prepare serum calibrators for CRP measurement.Methods Fresh serum without infectious diseases , hemolysis, lipemia and choloplania were collected and divided into 3 groups, low, medium, and high, according to the CRP concentration.Each serum pool was mixed , filtered, sterilized and aliquoted.The materials were tested for homogeneity and stability.The values of the CRP was assigned by particle enhanced immunonephelpmetry , and calibrated with international reference materials.The expanded uncertainty was evaluated.Results The materials were tested to be homogeneous (Ubb﹤Ur, P>0.05) with Ubb values being 0, 0.125, 0, Ur values being 0.046, 0.213, 0.785, and F values being 0.803, 1.686, 0.966 in CL, CM, CH groups respectively.Stability study, where F values are 0.609, 0.259, and 1.557 at 22-25℃, 1.217, 4.583, and 0.893 at 2-8℃(P>0.05), showed that the materials were stable for at least 3 days at 22-25 ℃or 30 days at 2-8 ℃, respectively.The certified values of the 3 levels materials for CRP were ( 2.64 ±0.14 ) , ( 31.17 ±0.63 ) , ( 73.85 ±1.74 ) mg/L, respectively.Conclusion The calibrators prepared for serum CRP measurement were homogeneous , stable and accurately assigned and can be used to calibrate the CRP measure system.