[Objective]To Study the sensitive sequence of MR for all stages of articular cartilage erosion and to evaluate the effect of MR on the correct diagnosis in the early stage of articular cartilage.[Method]One human fresh amputated knee joint and 4 normal pig knee joints were used to study and select the best sequence by T2WI,PDWI,GE,STIR,3D FS-FSPGR sequences and 6 pig oatcoarthritis(OA)model knee joints were studied by the selected best sequence,MRI was compared with correspont histological evidence to evaluate the effect of MR on the correct diagnosis of OA cartilage.[Result]The best results of tissue resolution and diagnosis rate were seen in 3D FS-FSPGR of all five sequences respectively 67.9% and 93.7%,of proteogly(PG)and collagen fiber(CF)mainly distributed in the deep cantilage.Dyeing of PG in diffent cantilage erosion was decreased while CF increasing.[Conclusion]3D FS-FSPGR is a favorable scan sequence to examine the OA changes of articular cartilage.MRI could show the pathological changes including early stage and have good veracity to the early pathological changes of OA cartilage.

[Objective]To compare the value of the MR imaging and arthroscopy for detecting articular cartilage erosion,and to evaluate the clinical effect of MR imaging on the correct diagnosis in the early stage of articular cartilage lesion.[Method]Twenty-six patients(27 knees)with persistent knee pain who were scheduled for arthroscopy underwent MR scanning,including 3D FS-FSPGR,FSE PDWI and FSE T1WI sequences.The results of each sequence were then compared with the arthroscopic findings.[Result]Using arthroscopic results as the standard of reference,the 3D FS-FSPGR images had the higher sensitivity(96.5%)and accuracy(95.0%)than the standard MR imaging,the 3D FS-FSPGR sequence was well consistent with the result of arthroscopic,Kappa value was higher(0.776)than the other sequences(P

Objective To investigate the repair effect of low intensity ultrasound on radioactive mandibule bone to provide new idea for prevention of osteoradionecrosis of the mandibule after irradiation.Methods The animal models of radioactive mandibular injury were made and treated with low intensity ultrasound.Low intensity ultrasound treatment group ( Group Ⅱ ) and not with low intensity ultrasound treatment group (Group Ⅰ ) were observed by microvessel density detection,micro-CT,ponceau trichrome stain and so on,and compared with control group.Then,the obtained data were statistically analyzed.Results The animal models of radioactive mandibular injury were successfully established.The microvessel density in Group Ⅱ was greater than that in Group Ⅰ.The bone volume fraction,trabecular thickness,bone surface/bone volume and the trabecular number in Group Ⅱ were significantly larger than those in Group Ⅰ (P ＜ 0.05).The ponceau trichrome staining showed that the osteocytes largely disappeared and that the cancellous bone trabecular atrophied in Group Ⅰ,while new bone formation,trabecular and numerous osteoblasts around trabecular were largely observed in Group Ⅱ.Conclusion Low intensity ultrasound has a good recovery effect on bone tissue after irradiation.

Objective: To analyze the relationship of school natural environment perception, exercise identification and exercise behavior, so as to provide references for promoting and improving exercise behavior among junior high school student. Methods: A total of 607 junior high school students were selected from Jiangsu, Anhui, Jiangxi, Jilin and Henan provinces by adopting a combination of convenience sampling and stratified cluster random sampling methods, and a longitudinal tracking survey was conducted over a threemonth period in two stages (T1: March 2023, T2: June 2023), from March to June 2023. Assessment was carried out by using School Natural Environment Perception Scale, Physical Exercise identification Scale and Physical Activity Rating Scale. The associations of school natural environment perception, exercise identification and exercise behavior were explored using correlation analysis and the crosslagged model. Results: For the junior high school students during T1 and T2 periods, there were positive correlations between the two of school natural environment perception, exercise identification and exercise behavior (r=0.04-0.61, P<0.01). The crosslagged analysis revealed that school natural environment perception in T1 period significantly predicted exercise identification (β=0.22, P<0.01) and exercise behavior (β=0.18, P<0.01) in T2 period. Moreover, the exercise identification in T1 period significantly predicted school natural environment perception (β=0.18，P<0.01) and exercise behavior in T2 period (β=0.19, P<0.01). However, the effects of exercise behavior on the school natural environment perception (β=0.03) and exercise identification (β=0.03) were not statistically significant (P>0.05). Conclusions For the junior high school students, school natural environment perception and exercise identification are the cause variables of the exercise behavior. Moreover, school natural environment perception and exercise identification have an interactive effect.

Objective To detect the level of CD4+ T cell-derived leptin(LP) in peripheral blood mononuclear cells(PBMC) from the patients with Hashimoto′s thyroiditis(HT) and to investigate its role in the occurrence and development of HT .Methods The peripheral blood samples in the patients with HT and the healthy controls were collected and separated for obtaining PBMC .The CD4+ T cells magnetic beads was adopted to separate CD4+ T cells for culturing in vitro .The LP level in supernatant was detected by ELISA .The relative expression amount of the orphan nuclear receptor RORγt was detected by real-time quantitative PCR (qRT-PCR) method .Results The LP level of CD4+ T cell culture liquid in the HT patients was markedly higher than that in the healthy control group ,difference was statistically significant (P<0 .05) .Furthermore ,the LP level in CD4+ T cell culture liquid was posi-tively related with the relative expression amount of RORγt in HT patients .Conclusion The CD4+ T cell-induced leptin level is in-creased in HT patients ,which is closely related with the occurrence and development of HT .

Objective: To investigate the temperature control methods for long-distance transportation of red blood cell suspensions in military affairs or emergence.Methods: We loaded blood transport cars with red blood cell suspensions to full capacity and kept the temperature by combined cooling methods.Results: The temperature in the container was kept between 2-6.2℃ during the 72 h transportation,even when the refrigeration system of the car was out of work.Conclusion: The required temperature for long-distance transportation of red blood cell suspensions could be attained by combined cooling methods in case of insecurity of transportation time and the effect of single cooling method.

By applying the methods of inter—element meter andmean line,decoction solution of 110 recipes were anal-ysed for the characteristics of distribution of inorganicelements.In those recipes containing more warm-heat drugs,the contents of most elements were higherthan the line of average value,while those with morecool—cold drugs,lower than the mean line,suggest-ing that the distribution value of inorganic elementsmay be a determining factor responsible for the cold-heat quality of a recipe.

s: Objective To explore the role of microRNA-206 in peripheral blood mononuclear cells (PBMC) in the pathogenesis and development of childhood asthma. Methods Twenty-seven asthmatic children and 25 healthy controls were enrolled in the study. Peripheral blood mononuclear cells were isolated in both healthy subjects and asthmatic children in acute attack and remission stages. Total RNAs were extracted from PBMC stimulated by PMA and ionomycin, and then the RNA was reversely transcribed into cDNA. The expressions of microRNA-206 and Kruppel-like factor 4 (KLF4) and IL-17 mRNA were detected by real-time quantitative PCR (qRT-PCR) method. Results There was signiifcant difference of microRNA-206 levels among asthmatic children in attack stage and in remission stage and normal controls (F=46.58~72.81, P=0.000). Through pair-wise comparison, the microRNA-206 levels of asthmatic children in attack stage were signiifcantly lower than those in remission stage and normal control groups (P<0.01). The KLF4 and IL-17 mRNA levels of asthmatic children in attack stage were signiif-cantly higher than those in remission stage and normal control groups (P<0.01). There was no signiifcant difference of miR-206, KLF4 and IL-17 mRNA between asthmatic children in remission stage and the healthy controls (P>0.05). Furthermore, a negative correlation was found between the expression of miR-206 and KLF4 (r=–0.66, P<0.01) and between the expression of miR-206 and IL-17 mRNA (r=–0.81, P<0.01) in asthmatic children in attack stage. A positive correlation was also found between KLF4 and IL-17 mRNA in asthmatic children in attack stage (r=0.70, P<0.01). Conclusions The expression of miR-206 is decreased in asthmatic children, and miR-206 might be involved in the pathogenesis and development of asthma.

Objective To explore the role of miR-206 in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients.Methods Human PBMCs were isolated by standard densitygradient centrifugation over Ficoll-Hypaque solution in SLE and healthy controls.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,thenthe RNA was transcribed reversely into cDNA.The expression of miR-206 and Kruppel-like factor 4 (KLF4) and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative polymerase chain reaction (qRT-PCR) method.T test and Spearman's correlation test were used for statistical analysis.Results The expression of miR-206 in the PBMCs of SLE patients was significantly decreased compared with that of healthy controls (0.066±0.021 vs 0.143±0.059,t=3.136,P＜0.01).The levels of KLF4 and RORγt mRNAin the PBMCs of SLE patients were increased significantly than those of healthy controls (0.637 ±0.186 vs 0.104 ± 0.028,t=6.673,P＜0.01),(0.575±0.263 vs 0.065±0.014,t=7.386,P＜0.01).Furthermore,there was a negative correlation between the expression of miR-206 and KLF4 or RORγt mRNA in SLE patients (r=-0.627,P＜ 0.01),(r=-0.853,P＜0.01).Conclusion These results indicate that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Thl7 cells in SLE patients.

Objective To explore the role of microRNA-206 (miR-206)in peripheral blood mononuclear cells (PBMC)from rheumatoid arthritis (RA)patients.Methods 27 patients with RA and 25 healthy controls were enrolled into the current study.Human peripheral blood mononuclear cells (PBMCs)were isolated by standard density-gradient centrifugation over Ficoll-Hypaque solution in rheumatoid arthritis and healthy control volunteers.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,then the RNA was transcribed reversely into cDNA.The expression of mi-croRNA-206 and Kruppel-like factor 4 (KLF4)and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative PCR (qRT-PCR)method.Student’s unpaired t-test and spearman correlation were used for statistica1 analysis. Results The expression of miR-206 in the PBMCs of RA patients was significantly decreased compared with that of the healthy controls (0.056±0.019 vs 0.138±0.057,t=3.103,P<0.01),The levels of KLF4 and RORγt mRNAin the PB-MCs of RA patients were increased significantly verves those of the healthy controls(0.604±0.183 vs 0.098±0.027,t=6.651,P<0.01;0.583±0.271 vs 0.069±0.018,t=7.438,P<0.01),Furthermore,a negative correlated between the ex-pression of miR-206 and KLF4 or RORγt mRNA in RA patients (r=-0.639,P<0.01;r=-0.842,P<0.01).Conclusion These results indicated that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Th17 cells in RA patients.