Objective: To select the optimum prepartion procedure for Sanhuangweixueping, Capsules. Methods: The optimum conditions were selected with the contents of active components: emodin, berberine, and baicalin as markers. Results: The optimum preparation procedure was: berberine was extracted with 95% alcohol. Rhizoma Bletillae was immersed with water, then precipitated with 70% alcohol. Radix et Rhizoma Rhei, Radix Scutellariae and Radix Glycyrrhizae were decocted, then precipitaed with 60% alcohol. Sanhuangweixueping Capsules prepared with the optimum preparation procedure possessed stronger pharmacological effect. Conclusion: The optimum prepartion procedure is reliable and the extraction ratio of active compoents is high.

Objective: To study the effect of zinc supplement on bone-contacting implant surface ratio (BCSR).Methods: 40 rabbits with titanium implants placed into the proximal tibial heads were divided randomly into zinc supplement group(n=20) and control group(n=20). 1% zinc sulfate in 0.9% saline was administered intramuscularly at the dose of 4 mg/(kg?d) for each tested animal, and the same amount of 0.9% saline was used for the controls. The animals were sacrificed 1, 2, 4, 8 and 12 weeks after treatment respectively.BCSR,bone zinc content and serum Cu/Zn values were measured with morphometry, atomic absorption photometry and atomic nebulizntion absorption method respectively.Results: Zinc supplement could significantly increase the BCSR value (P0.05). Conclusion: Moderate zinc supplement may increase the bone-contacting implant surface ratio, but not alter the zinc metabolism.

There was almost one hundred years since surgical stapler invention to now. Using stapler (stitching instrument) in surgery has many advantages, such as stitching fast, easy to operating, few side effects and complications. It makes tumor excision surgery can’t be cut focus in past possible and improving the curative effect of the surgery. Surgical staplers also give difficult surgery and new technology strong supports. In the last ten years, for cheap price and rapid development of the technology level, domestic staplers’ market share is increasing year by year and breaking the foreign monopoly product. In open surgery, domestic stapler has progressively more than foreign brands. In endoscopy surgery, domestic staplers also have a place. In current, surgical staplers are used more and more popular in the domestic tertiary medical institutions and becoming indispensable tools in many surgeries. Surgical staplers (stitching instrument) are broadly used in esophageal reconstruction, gastrointestinal tract reconstruction, endoscopic surgery in pulmonary bulla resection, uterus resection, pancreas resection, duodenum resection

Objective To discuss the relationship between FokI gene polymorphism and colorectal canc-er.Method The references that met the inclusive criteria were assessed .The extracted data were summarized in the form of tables .Data were analyzed by heterogeneity test .Meta-analysis was conducted by using fixed effects model or random effects model .Results Fifteen experimental studies were included in this study ,including 7, 859 colorectal cancer patients and 9,933 controls.The association between VDR gene FokI and colorectal cancer was expressed as the odds ratio(OR)and 95% confidence interval(CI).FokI recessive genetic model,ff versus FF+Ff,ORrecessive=1.01(95% CI=0.87~1.17,I2 =54%,P=0.91),ORdominant =0.94(95% CI=0.83~1.06,I2 =64%,P=0.30);allele model,fversus F,OR=0.98(95% CI=0.89~1.08,I2 =71%,P=0.72).In subgroup analysis,recessive genetic model,ORCaucasian race =0.95(95% CI=0.86~1.05,I2 =0%,P=0.33);ORyellow race=1.12(95%CI=0.77~1.63,I2 =72%,P=0.54).In subgroup analysis,dom-inant gene model,ORCaucasian race =0.95(95% CI=0.86~1.04,I2 =27%,P=0.29);ORyellow race =0.89(95%CI=0.65~1.22,I2 =78%,P=0.47).In subgroup analysis,allele model,ORCaucasian race=0.97 (95%CI=0.90~1.04,I2 =31%,P=0.38);ORyellow race =1(95% CI =0.79 ~1.27,I2 =82%,P=1.00).Conclusion Our results suggested that in VDR gene FokI ,F and f alleles were not obviously directly correlated with colorectal cancer .No difference was found between Asian and Caucasian .The conclusions in other populations should be further identified by extensive experimental studies .

Objective: To prepare human cementum extract and to assess its protein content. Methods: Human permanent teeth were collected from the oral surgery clinic. Cementum was obtained from freshly extracted healthy teeth without inflammation.Connective tissue adhereing to the root surfaces was removed, and cementum was harvested with a curette. Then the cementum was treated by CH 3COOH and guanidine respectively to obtain two extracts. Protein content was assessed by sodium dodecyl sulphate ( SDS) polyacrylamide electrophoresis. Results: Extract obtained from CH 3COOH treatment consisted of two kind of proteins ( M r 55 000 and M r 68 000); that of guanidine consisted of four kind of proteins ( M r 62 000, M r 60 000 , M r 5 5000 and M r 41 000).Conclusion: The methods used is an applicable way to prepare cememtum extracts.

Objective: To study the expressien and heterogeneity of epidenmoid growth factor receptor(EGFR) in human mucoepidermoid carcinoma MEC 1 cells. Methods: EGFR1 McAb (MaH) immunofluorescent stain and flowcytometer (FCM) were used to detect the positive percentage and heterogeneity of EGFR expression in MEC 1 cells. Results: Positive expression of EGFR was found in 100% of MEC 1 cells. Average peak channel of relative fluorescent intensity was 8.006?4.410. Double peaks of MEC 1 cells from single parameter histogram was observed with FCM. Conclusion: All MEC 1 cells express EGFR and there are heterogeneous cell populations of EGFR expression in MEC 1 cell line.

Objective: To observe the interface bonding between the coral and new bone. Methods: The block coral was implanted on rabbit calvaria subperiosteally. The specimens were observed by the scanning electron microscopy 2, 4, 8, 12 weeks after operation respectively. Results: The coral was progressively absorbed and replaced by new bone. The coral bond to bone directly. No intervening layer was found between coral and bone. Conclusion: The coral bond to bone by direct mechanical way. [

Objective To investigate effect of laparoscope anesthesia inducted by sevoflurane combined with different doses of propofol on vascular endothelial growth factor-C (VEGF-C) and prostaglandin E2(PGE2)in patients with hysteromyoma.Methods 77 cases of patients with uterine fibroids were selected and divided into two groups.40 cases in group A were anesthetized inducted by 2.0 mg/kg propofol combined with sevoflurane, 37cases in group A were anesthesia inducted by 1.0 mg/kg propofol combined with sevoflurane.VEGF-C and PGE2 in serum were compared before and 48h after the operation.Results Compared with preoperation, VEGF-C and PGE2in serum were lower (P<0.05), VAS score was lower (P<0.05);Compared with group B, VEGF-C and PGE2 in serum of the group A were lower (P<0.05), VAS score was higher (P<0.05).Conclusion Different doses of propofol on serum VEGF-C and PGE2 in patients with laparoscopic uterine fibroids have different effects, 1.0 mg/kg has a sedative effect, 2 0 mg/kg with anesthesia induction.

Objective To investigate the mechanisms of mesenchymal stromal cells (MSCs) in promoting the transplanted islets neovascularization.Methods We performed co-transplantation of allogeneic islet and MSCs into non-obese diabetic (NOD) mice and the lymphocyte chimeric rate was detected by flow cytometry.The MSCs migration,transformation and secretion of cytokines were detected by immunofluorescence.Results In mice of co-combination transplantation of islet with MSCs,donor lymphocytic chimerism in graft was increased.BM-MSCs can migrate to transplanted islet,differentiate into vascular smooth muscle cells,and also secrete vascular endothelial growth factor (VEGF).Conclusions MSCs can migrate to transplanted islet and promote neovascularization.Also,it enhanced allograft immune tolerance of islet grafts via increasing donor lymphocytic chimerism.

objective: To produce titanium dioxide hydrogel layer on the pure rough titanium(Ti) surface. Methods: Pure Ti discs( d =15mm) were polished to 600 grind, sandblasted and etched with acid, socked in 60 ℃ 5 mol/L NaOH solution for 24 h and then heated to 600℃ for an hour to get goughening, alkaline and heat treated Ti(RAH). The smooth, rough and RAH surfaces were analyzed with scanning electron microscope(SEM) and energy despersive X ray analysis(EDXA). Results: On smooth surfaces only regular polished grooves could be seen, while holes in different diameters formed on rough surfaces. A porous framework was found on the surfaces of the RAH observed by SEM. EDXA showed that the smooth and rough surfaces were consisted of 100% Ti, while there were a lot ofoxygen and sodium in RAH discs. Apatite formed on the RAH Ti surfaces when it is soaked in simulated body fluid for two weeks. Conclusion: A porous Ti dioxide hydrogel layer forms on the rough titanium substrate during alkaline and heat treatment.