Objective To examine the expression change of HIF-1α and VEGF in protein levels and the corelation of them in Q8C939 cell line under chemical hypoxia induced by CoCl2 in vitro. Methods ELISA and immunohistochemical technique were used to examine the change of VEGF and HIF-1α protein level in QBC-939 line in different concentrations (200, 150, 100, 50, 0 μmol/L) of COCl2 and different periods (6 h, 12 h, 24 h, 48 h) in hypoxia. Results CoCl2 can up-regulate the expression of HIF-1α and VEGF at least partly in a dose and time dependent pattern. ELISA demonstrated that the level of VEGF protein in different concentrations and different hypoxia periods, the result showed that the VEGF protein was in different concentration groups over contrast group (P<0.05). The VEGF protein also increased continuously with the growing of hypoxia time (P<0.05). Immunohistochemical detection of concentrations of different groups, HIF-1α, VEGF protein in the average gray compared with the control group was different(P<0.05). Different time periods of hypoxia HIF-1α, VEGF protein in the average gray, any two group comparisons have difference (P<0.05). Different concentrations, the normal control group, different time period HIF-1α and VEGF protein expression of relevance were analyzed. The correlation coefficient was 0.830, P <0.01, 0.909, P <0.01. Conclusion In QBC939 cell line of hypoxia, there is up-regulation HIF-1α and VEGF.HIF-1α regulated the expression of VEGF.

Objective To investigate the relationship between the mechanism of mitigation of neuropathic pain by spinal cord stimulation (SCS) and high mobility group protein box-1 (HMGB1)/Toll-like receptor 4 (TLR4)/NF-κB signaling pathway in the spinal cord of rats.Methods Forty-eight male Sprague-Dawley rats,aged 8 weeks,weighing 250-300 g,were randomly divided into 4 groups (n =12 each) using a random number table:sham operation group (S group),chronic constrictive injury (CCI) group,sham SCS group (S-SCS group),and SCS group.Neuropathic pain was induced by CCI in the animals anesthetized with intraperitoneal chloral hydrate.The sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread.Electrodes were placed into the epidural space at 5 days after CCI in S-SCS and SCS groups,and in addition SCS was performed at 12-14 days after CCI in SCS group.Paw withdrawal threshold to mechanical stimulation (MWT) were measured at 1 day before operation and 1,4,7,14 days after CCI.After measurement of pain threshold at day 14 after CCI,the animals were sacrificed and the lumbar segments (L4-6) of the spinal cord were obtained for determination of mRNA expression of HMGB1,TLR4,and NF-κB p65 (in the nuclear protein,by real-time fluorescent quantitative PCR),protein expression of TLR4 and NF-κBp65 (by Western blot),and protein expression of HMGB1 (by immunohistochemistry).Results Compared with S group,MWT was significantly decreased after CCI,the mRNA and protein expression of HMGB1 and TLR4 was up-regulated,and the expression of NF-κB p65in the nuclear protein and mRNA was up-regulated in CCI,S-SCS and SCS groups.Compared with CCI group,MWT was significantly increased after spinal cord stimulation,the mRNA and protein expression of HMGB1 and TLR4 was down-regulated,and the expression of NF-κB p65 in the nuclear protein and mRNA was down-regulated in SCS group.Conclusion The mechanism by which SCS mitigates neuropathic pain may be related to inhibition of HMGB1/TLR4/NF-κB signaling pathway in the spinal cord of rats.

Objective To evaluate the effect of spinal cord stimulation (SCS) on the expression of spinal substance P (SP) and calcitonin gene-related peptide (CGRP) in rats with neuropathic pain.Methods Forty-eight male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were randomly divided into 4 groups (n =12 each) using a random number table: sham operation group (S group) , n europathic pain group (group NP), sham electrical stimulation group (N-SCS group) , and SCS group.Neuropathic pain was induced by chronic constriction injury (CCI) in anesthetized rats.The sciatic nerve was exposed, and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 chromic catgut.Electrodes were placed into the epidural space at 5 days after CCI in N-SCS and SCS groups, and in addition, SCS was performed at 12-14 days after CCI in SCS group.The mechanical paw withdrawal threshold (MWT) was measured at 1 day before CCI, and 1, 4, 7 and 14 days after CCI.After measurement of pain threshold at day 14 after CCI, the animals were sacrificed, and the lumbar segments (L4-6) of the spinal cord were obtained for determination of the expression of SP and CGRP in the spinal cord by immunohistochemistry.Results Compared with group S, the MWT was significantly decreased, and the expression of SP and CGRP protein and mRNA was up-regulated in NP, N-SCS and SCS groups (P＜0.05).Compared with group NP, the MWT was significantly increased, and the expression of SP and CGRP protein and mRNA was down-regulated in group SCS (P＜0.05).Conclusion The mechanism by which SCS mitigates neuropathic pain may be related to down-regulated expression of SP and CGRP in the spinal cord of rats.

Objective To summarize the nursing experience of a child with serious skin problems at the puncturing site of peripherally inserted central catheter (PICC) for methotrexate (MTX) infusion.MethodsA pediatric patient from the Hematology Ward of Beijing University People''s Hospital was found to be with serious skin problems at the puncturing site of PICC for MTX infusion.Local irradiation with ultraviolet, disinfection of local skin with iodine volts and 0.9％ sodium chloride injection, coverage of the broken skin with alginate dressing, and fixation of PICC with sterile gauzes were applied.Results After 22 days of nursing, the pain and itching at the broken skin around PICC disappeared.The skin returned normal, without exudates and prolapse of catheter.Conclusions After MTX use via PICC, the children can easily become irritable due to local symptoms and their own feelings after skin allergy.Meanwhile, prolonged treatment and nursing may make the child cry and increase the stress of parents.Caregivers needs to explain the related factors of allergy to the children and their families, and give the correct nursing intervention, so as to improve local allergy and facilitate the retention of PICC.

Objective:To Observe the effect of recombinant tissue-type plasminogen activator (rt-PA) in treatment of acute myocardial infarct.Methods:Two groups of patients were observed.The subjects in the control group (35 patients) were treated with routine intervention,while those in thrombolysis group (15 patients) were treated with rt-PA in addition to the routine intervention.Results:There is a better outcome in the thrombolysis group as compared with that in the control group,in terms of length of angina relief,degree and length of EKG changes as well as the time for serum enzyme changes.The mortality rate in the thrombolysis group was significantly lower than that in the control group (P＜0.01).Conclusion:The rt-PA for thrombolysis in acute myocardial infarct patients can reduce the infarct size and improve the cardiac function rapidly as well as increase the survival rate.

Chinese herbal medicine (CHM) has been widely used in the treatment of multiple sclerosis (MS). However, there is no systematic review to assess the efficacy and safety of CHM.

Objective To setup a measurement of human bone marrow micromegakaryocyte which based on CD41a and PI double‐labeled flow cytometric analysis ,and study the significance in the diagnosis of MDS .Methods In 42 cases of MDS patients , their bone marrow megakaryocytes were obtained by Percoll density gradient separation medium .The megakaryocyte glycoproteinⅡb/Ⅲa(CD41a)were marked with fluorescein isothiocyanate through its corresponding monoclonal antibody ,and their DNA were marked with PI .Then the megakaryocyte ploidy was analyzed by flow cytometry(FCM ) .Results The method for micromegakaryo‐cyte identification and analysis was established .In 42 patients with MDS ,the detection rate of micromegakaryocyte was 90 .5 per‐cent by FCM analysis ,but only 54 .8 percent by Wright‐Giemsa staining test and 64 .3 percent by immunohistochemistry ,the differ‐ence among them was statistically significant(χ2 = 13 .640 ,P= 0 .001) .The 42 patients with MDS were divided into two groups (low‐risk group and high‐risk group) .The detection rates of micromegakaryocyte were 81 .8 percent in low‐risk group and 100 per‐cent in high‐risk group separately by FCM analysis ,the difference was statistically significant(χ2 =4 .019 ,P=0 .045) .Conclusion The detection rate of micromegakaryocyte by FCM with CD41a and PI double marker is higher than that by cytochemical staining . The detection rate of micromegakaryocyte in the high‐risk group is higher than that of the low‐risk group ,which shows that the de‐tection of micromegakaryocyte is of great significance for MDS prognosis assessment .

AIM: To investigate the relationship between expression of Bmi-1(B cell-specific MLV integration site-1) in gastric cancer and its clinicopathologic significance.METHODS: 146 surgical patients with gastric carcinoma were followed up at least 2 years.Expression of Bmi-1 protein was examined by immunohistochemistry in their archival paraffin embedded tissue specimens.RESULTS: The intensive positive rate of Bmi-1 expression in gastric cancer was 67.8%(99/146).Expression of Bmi-1 was highly correlated with tumor size,clinical stage,lymph node metastasis and T classification(P0.05).The survival rate in the patients with Bmi-1 expression was much lower than that in those patients without Bmi-1 expression(P

OBJECTIVETo develop a LC-MS/MS method for determination of astragalin in rat plasma and study its pharmacokinetics in rats.

METHODSThe analytical column was packed with ZORBAX SB-C18, and the mobile phase was methanol-water containing 10 mmol/L ammonium acetate-formic acid (80:20:0.15, V/V/V). Quercetin was used as the internal standard (IS). Multiple reaction monitoring (MRM) mode was employed, and the transition of m/z was m/z 449.1→m/z 287.1 for astragalin, and m/z 301.1→m/z 151.1 for IS.

RESULTSA good liner relationship was obtained within the range of 1.00-1000 ng/ml (r(2)=0.9929), and the lower limit of quantification of astragalin was 1.00 ng/ml in rat plasma. The extraction recoveries were all above 93%. After oral administration of astragalin, the maximum plasma concentration of astragalin was 231.1∓67.3 ng/ml and the time to reach this value was 0.5∓0.1 h, with a half-life of 3.9∓1.3 h and an AUC of 782.6∓152.8 ng·h/ml.

CONCLUSIONThe method is highly sensitive, selective and rapid for determination of the concentration of astragalin in rat plasma to facilitates the study of its pharmacokinetics.

Objective: To investigate the health-seeking intention for urinary incontinence among adult women in Gansu Province, so as to provide insights into the management of female urinary incontinence. Methods : Women at ages of 20 years and older who lived in 8 communities and 8 villages of Gansu Province for at least one year were recruited using the multi-stage stratified cluster random sampling method. A face-to-face questionnaire survey was conducted from October 2019 to February 2020, and the demographic features, urinary incontinence status and health-seeking intention were collected and descriptively analyzed. Results: A total of 3 580 questionnaires were allocated and 3 485 were recovered, with a recovery rate of 97.35%. The respondents had a mean age of ( 51.18±17.13 ) years, with 1 759 respondents ( 50.47% ) that lived in urban areas, and 1 726 ( 49.53% ) that lived in rural areas. There were 1 150 respondents with self-reported urinary incontinence ( 33.00% prevalence ), including 340 cases with stress urinary incontinence ( 29.57% ), 78 cases with urge urinary incontinence ( 6.78% ) and 732 cases with mixed urinary incontinence ( 63.65% ). The overall proportion of health-seeking intention for urinary incontinence was 41.57%, and the proportions of health-seeking intention were 51.76%, 39.74% and 37.02% for stress, urge and mixed urinary incontinence, respectively. The proportion of health-seeking intention for urinary incontinence appeared a tendency towards a decline with the increase in household monthly income per capita and frequency of urinary leakage, and appeared a tendency towards a rise with the increase in educational levels ( P<0.05 ). In addition, a higher proportion of health-seeking intention for urinary incontinence was seen in women living in urban areas than in rural areas (5 3.63% vs. 31.98%, P<0.05 ), and a higher proportion was found in women with mental labors than in those with physical labors ( 60.81% vs. 40.24%, P<0.05 ), while a higher proportion was found in married women than in divorced or widowed women ( 44.33% vs. 23.53%, P<0.05 ). Conclusions There is a low proportion of health-seeking intention for urinary incontinence among adult women in Gansu Province. Age, occupation, place of residence, educational level, income, marital status, and frequency of urine leakage may affect the intention to seek medical care for urinary incontinence among adult women.