Objective To evaluate the effect of sofren injection on acute lung injury (ALI) in septic mice.Methods Ninety-six pathogen-free made Kunming mice,aged 8-10 weeks,weighing 30-35 g,were divided into 4 groups (n =24 each) using a randon number table:sham operation group (group Sham),ALI group,vehicle group (group Vehicle) and sofren injection group (group Sofren).Sepsis was induced by cecal ligation and puncture.In Vehicle and Sofren groups,double distilled water and sofren injection 4 ml/kg were intraperitoneally injected,respectively,after the model was established,followed by repeated admninistration once every 12 h.At 12,24 and 48 h after operation,the animals were sacrificed and lungs were removed for mnicroscopic examination and for determination of wet to dry weight ratio (W/D ratio),malondialdehyde (MDA) content,superoxide dismutase (SOD) activity,and contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in lung tissues (by enzyme-linked immunosorbent assay).The lung injury score (LIS) was calculated after haematoxylin and eosin staining.Results Compared with group Sham,the LIS,W/D ratio and contents of MDA,TNF-α and IL-6 were significantly increased,and the SOD activity was decreased at each time point in ALI and Vehicle groups (P＜0.05).Compared with group ALI,the LIS,W/D ratio and contents of MDA,TNF-α and IL-6 were significantly decreased,and the SOD activity was increased at each time point in group Sofren (P＜0.05),and no significant change was found in each parameter mentioned above in group Vehicle (P＞0.05).Conclusion Sofren injection can attenuate ALI in septic mice.

Objective To observe the clinical effect of ulinastatin(UTI)combined with thymosin alpha 1(Tα1)in the treatment of septic shock.Methods A retrospective analysis was conducted on the clinical data of 88 patients with septic shock admitted to our hospital from June 2021 to October 2023.The patients were divided into UTI group and UTI+Tα1 group according to different treatment methods,with 44 patients in each group.The treatment effects,clinical indicators,microcirculatory perfusion indicators[central venous oxygen saturation(ScvO2),lactate(LAC),capillary refill time(CRT),mean arterial pressure(MAP)],Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE Ⅱ)score,Sequential Organ Failure Assessment(SOFA)score,immune indicators,plas-ma and serum inflammatory indicators[soluble triggering receptor expressed on myeloid cells-1(sTREM-1),procalcitonin(PCT),interleukin(IL)-6,tumor necrosis factor-α(TNF-α)],and prognosis were compared between the two groups.Results After 7 days of treatment,the effective rate of treatment in the UTI+Tα1 group was higher than that in the UTI group(P＜0.05).The dura-tion of vasoactive drug use,mechanical ventilation time,ICU stay,and hospital stay were shorter in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 24 and 72 hours of treatment,ScvO2 and MAP gradually increased,LAC gradually decreased,and CRT gradually shortened in both groups(P＜0.05).After 24 hours of treatment,ScvO2 and MAP were higher,and CRT was shorter in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 72 hours of treatment,CRT was shorter,and MAP was higher in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,both APACHE Ⅱ and SOFA scores decreased in both groups compared to treatment before,and their scores were lower in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,the levels of CD3+and CD4+T lymphocytes increased in both groups compared to treatment before,and the levels were higher in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,the levels of sTREM-1,PCT,IL-6,and TNF-α decreased in both groups compared to treatment before,and the levels were lower in the UTI+Tα1 group than those in the UTI group(P＜0.05).After a 28-day follow-up,there was no sta-tistically significant difference in mortality between the two groups(P=0.398).Conclusion UTI combined with Tα1 can effectively promote the recovery of patients with septic shock,improve micro-circulatory perfusion,reduce plasma sTREM-1 and serum PCT levels,inhibit inflammatory responses,and improve prognosis.

Objective To observe the clinical effect of ulinastatin(UTI)combined with thymosin alpha 1(Tα1)in the treatment of septic shock.Methods A retrospective analysis was conducted on the clinical data of 88 patients with septic shock admitted to our hospital from June 2021 to October 2023.The patients were divided into UTI group and UTI+Tα1 group according to different treatment methods,with 44 patients in each group.The treatment effects,clinical indicators,microcirculatory perfusion indicators[central venous oxygen saturation(ScvO2),lactate(LAC),capillary refill time(CRT),mean arterial pressure(MAP)],Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE Ⅱ)score,Sequential Organ Failure Assessment(SOFA)score,immune indicators,plas-ma and serum inflammatory indicators[soluble triggering receptor expressed on myeloid cells-1(sTREM-1),procalcitonin(PCT),interleukin(IL)-6,tumor necrosis factor-α(TNF-α)],and prognosis were compared between the two groups.Results After 7 days of treatment,the effective rate of treatment in the UTI+Tα1 group was higher than that in the UTI group(P＜0.05).The dura-tion of vasoactive drug use,mechanical ventilation time,ICU stay,and hospital stay were shorter in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 24 and 72 hours of treatment,ScvO2 and MAP gradually increased,LAC gradually decreased,and CRT gradually shortened in both groups(P＜0.05).After 24 hours of treatment,ScvO2 and MAP were higher,and CRT was shorter in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 72 hours of treatment,CRT was shorter,and MAP was higher in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,both APACHE Ⅱ and SOFA scores decreased in both groups compared to treatment before,and their scores were lower in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,the levels of CD3+and CD4+T lymphocytes increased in both groups compared to treatment before,and the levels were higher in the UTI+Tα1 group than those in the UTI group(P＜0.05).After 7 days of treatment,the levels of sTREM-1,PCT,IL-6,and TNF-α decreased in both groups compared to treatment before,and the levels were lower in the UTI+Tα1 group than those in the UTI group(P＜0.05).After a 28-day follow-up,there was no sta-tistically significant difference in mortality between the two groups(P=0.398).Conclusion UTI combined with Tα1 can effectively promote the recovery of patients with septic shock,improve micro-circulatory perfusion,reduce plasma sTREM-1 and serum PCT levels,inhibit inflammatory responses,and improve prognosis.