BACKGROUND:Regulation of neural stem cells differentiation hampers its application,and the regulation mechanism remains poorly understood.Anterior subventricular zone(SVZa)is one of the rich zones of neural stem cells.The neural stem cells at SVZa can immigrate for a long distance with undifferentiated states and finally differentiate into intemeurons at the olfactory bulbs.Bone morphogenetic protein-2(BMP2)and DLX5 play a significant role in immigration and differentiation of neural stem cells at SVZa towards olfactory bulb.OBJECTIVE:To study the feature of construction and immigration of neural stem cells at SVZa,and to review the role of BMP2 and DLX5 in neural stem cells differentiation,in addition,to explore the regulation mechanisms of neural stern cells differentiation.METHODS:Literatures from PubMed database(http://www.ncbi.nlm.nih.gov/PubMed),CNKI database(www.cnki.net/index.htm)and WANFANG database(http://www.wanfangdata.com.cn)published between January 1997 and January 2009 were searched with the key words of"neural stem cells,SVZa,BMP2,and DLX5".The repetitive and obsolete studies were excluded.RESULTS AND CONCLUSION:A total of 121 literatures were selected and primarily collected,including 86 Chinese literatures and 35 English literatures.Finally,according to standardization,28 literatures were further analyzed.Neural stem cells of SVZa have unique construction and can immigrate for a long distance.BMP2 and DLX5 play a significant role in neuronal immigration and differentiation of the NSCs of SVZa,but the detailed mechanism of BMP2 and DLX5 in SVZa is not clear.

Objective:To comprehensively evaluate the quality of Herba Clematidis Intricatae through HPLC multi-index components, chemometrics combined with EW-TOPSIS. Methods:A total of 18 batches of Herba Clematidis Intricatae samples from seven provinces were collected. Contents of luteolin-7-O-glucoside, rutoside, hyperoside, quercitrin, quercetin, luteolin, apigenin and kaempferol in Herba Clematidis Intricatae were simultaneously determined by HPLC. Chemometrics method was used to comprehensively analyze the content determination results, and the main potential markers affecting the quality of Herba Clematidis Intricatae were analyzed. The quality of Herba Clematidis Intricatae from different origins was evaluated. Results:The eight components showed good linear relationships within their respective ranges ( r≥0.999 1), and accuracy was good ( RSD<2.0%). The chemometrics method showed that 18 batches of Herba Clematidis Intricatae could be clustered into 3 categories, showing certain regional differences. Rutoside, hyperoside and luteolin-7-O-glucoside were the indicative components affecting the difference of chemical constituents in Herba Clematidis Intricatae; results of EW-TOPSIS method showed that the optimum quality of Herba Clematidis Intricatae from Inner Mongolia and Liaoning, followed by those of Hebei, Shanxi and Shanxi, and lowest in Qinghai and Gansu. Conclusion:The established HPLC method is convenient and accurate, and combined with chemometrics and EW-TOPSIS method can be used for the comprehensive evaluation of the quality of Herba Clematidis Intricatae.