Objective To study the changes of the cellular composition in peripheral blood stem cell transplantation(PBSCT) donor with granulocyte colony stimulating factor(G-CSF)and the short-term influence of G-CSF on the donors.Methods Eighteen donors of PBSCT were administrated with G-CSF,5-10?g/(kg?d),for 4-5d.Peripheral blood mononuclear cells (MNC) were collected after the administration of the G-CSF and analyzed by flow cytometry.The side effects of G-CSF on donors were also studied.Results The peripheral white blood cell (PWBC) counts peaked on day 4 or 5 after mobilization.Compared with the number of WBC before mobilization,it increased 7 to 14 times after mobilization (P

Group-A rotaviruses are recognized as the most common cause of acute diarrhea. Phylogenetic analyses of the VP7 genes of rotaviruses circulating in Nanjing (China) could aid in the development of rotavirus vaccines. A total of 908 stool specimens were collected from patients suffering from acute diarrhea in Nanjing between October 2012 and December 2013, and were tested further for rotaviruses. Fifty rotavirus isolates selected randomly were typed by reverse transcription-polymerase chain reaction using serotype-specific primers for G genotyping. VP7 genes of 19 G9 strains were sequenced for further genetic characterization. Among the 908 stool specimens examined during the surveillance period, 103 (11.34%) were rotavirus-positive. G9 was the most predominant genotype (78.0%), followed by G2, G1 and G3. Sequence and phylogenetic analyses of the VP7 genes of serotype G9 rotaviruses revealed these strains to comprise two lineages (G9-VI, G9-III) and to be dominated by the G9-VI lineage (which belonged to a unique subcluster of Japanese and Chinese G9 strains). Amino-acid sequences of the four antigenic regions (A, B, C or F) were variant among a portion of strains, which may have contributed to the prevalence of G9 rotaviruses in this area.
Adult ; Amino Acid Sequence ; Antigens, Viral ; chemistry ; genetics ; Capsid Proteins ; chemistry ; genetics ; China ; Evolution, Molecular ; Humans ; Infant ; Molecular Sequence Data ; Mutation ; Phylogeny ; Rotavirus ; genetics ; immunology ; physiology ; Serogroup