7-Bromethyoxybenzentetrahydropalmatine ( EBP) possessed antiarr-hythmic effect in several animals models.The authors studied the direct influence of EBP on the automaticity, refractory period, excitability and the contractility of isolated papillary muscle and atrium of guinea pig.EBP was found to decrease the automaticity and prolong the functional refractory period and increase the contractility of guinea pig papillary muscle, but it exerted no influence on its excitability.The decrease in automoticity and prolongation of refractory period was thought to be ascribed to its antiarrhythmic action.

AIM To observe the anti hyperglycemic effect of sodium orthovanadate on type 2 diabetes rats. METHODS Rats were fed by highly fatted food to grow into obesity. Then values of plasma free fatty acid (FFA) were tested. Glucose disposal was measured with the hyperinsulinemic euglycemic clamp technique to assess insulin resistance in vivo. Small dose of streptozocin was injected ip with insulin resistance. Those with plasma glucose over 11 1 mmol?L -1 were assigned to type 2 diabetes model group, continually fed with sodium orthovanadate for 3 days and assayed the values of plasma glucose in fasting state. RESULTS ①Rats fed with fatted food needed more insulin to maintain normal plasma glucose, ie (0 54?0 02) U?min -1 , higher than that of normal control ( P

Aim To establish the technology of isolation rat’s mesenteri c lymphatics and the culture method of their endothelium and confirm the ability o f synthesis nitric oxide and Factor Ⅷ related antigens in the endothelium in vitro. Methods With an operating microscope and microsurgical in struments, rat’s mesenteric lymphatics were isolated and the endothelium were p rimarily cultured and subcultured. Exerting immunohistochemical method, Factor Ⅷ related antigens and nitric oxide synthase were evaluationed. The contents of NO metabolite were measured. Results The rat’s mesenteric lympha tics were isolated and the endothelium were Fusiform shape or polygon, the monol ayer cultures displayed typical cobblestone or pavingstone morphology. Factor Ⅷ related antigens were expressed and their positive rate was 98%. The positive rate of nitric oxide synthase was 83%. Positive reaction products were buffy ar ound nuclear. NO metabolite was (10.26?2.17) mol?L -1(n=7). Conclusion The culture method we introduced was very practical, simple and reliable. The endothelium is in normal structure and function characters. It also certificated that the endothelium in vitro could express nitric oxide synthase and Factor Ⅷ related antigens.

Aim To investigate the effects of 4-aminopyridine (4-AP),K+ channel blocker, on the gastrointestinal function of murine. Methods Charcoal suspension was used to detect the effects of 4-AP on gastrointestinal motility of mice in vivo, contraction of isolated fundus longitudinal strips and duodenum of rats were studied in vitro,gastric acid secretion was measured by Gastric Secretion Test. Results 4-AP(5 mg?kg -1,ig)inhibited the gastrointestinal motility of mice. 4-AP(5 mmol?L -1) increased the maximum contractive force and minimum relaxation force, decreased the amplitude and frequency of the isolated duodenum peristaltic contraction. 4-AP(2.5 mg?kg -1,ip) significantly enhanced gastric acid secretion of rats. Conclusion 4-AP inhibited gastrointestinal motility and enhanced gastric acid secretion in murine.

HERG/IKr plays an important role in repolarization of cardiac action potential. In pathological condition such as cardiac ischemia, congestive heart failure and myocardial hypertrophy, the density and kinetic properties of HERG/IKr will change. This review is about modulation of HERG/IKr in pathological condition and effect of traditional chinese medicine on HERG/IKr.

Translational medicine is a novel concept about combination of basic research and clinical application. The aim of translational medicine is to realize the translation of basic research into clinical practice. microRNAs (miRNAs) are non-coding single-stranded RNAs with 21-25 nucleotides in length as newly discovered factors in regulating gene expression. Recently, the key regulatory role of miRNA in the cardiovascular system has been elucidated and amount of remarkable results has been achieved, particularly in the regulation of cardiac arrhythmias. A series of studies demonstrate that miRNAs are involved in the regulation of expression of a variety of proteins associated with cardiac electrical activity, and are the potential targets of occurrence of cardiac arrhythmias and anti-arrhythmic drugs. miRNAs as a therapeutic target regulate the stability of mRNAs of target genes or play an inhibitory role in the translation process. Stability of the corresponding miRNA expression levels in the target organ may be a new approach for the disease therapy. Regarding the dysfunction of miRNA, we employed miRNA re-expression strategy and anti-miRNA strategy to correct target protein function and provide a new entry for the therapy of arrhythmia. With the technology of miRNA mimics and antagomirs, miRNAs are expected to treat various cardiovascular diseases and will provide a fresh impetus to achieve transform medicine.

Objective To explore the clinical effect of Tuina therapy for knee osteoarthritis (KOA) patients and their musculus quadriceps fexoris strength. Methods The 114 patients with KOA who met the inclusion criteria were randomly divided into the observation group and the control group (57 in each group). The patients in observation group were treated with Tuina therapy, and the control group were treated with Meloxicam. All the patients were treated for 30 days. The Isokinetic muscle strength test was used to evaluate the strength of the four biceps femoris muscle, and HSS score and pain score were observed before and after treatment. Results The total effective rate of the observation group was 91.2% (52/57), and the control group was 71.9% (41/57). The difference between 2 groups was statistically significant (χ2=3.971, P=0.018). After treatment, the HSS scores (79.0 ± 2.0 vs. 66.3 ± 1.4; t=3.121, P<0.01) in observation group were significantly higher than that in control group, and the pain scores (1.7 ± 1.1 vs. 3.2 ± 1.1; t=2.953, P<0.01) in observation group were significantly lower than that in control group. The PT (59.91 ± 25.90 Kgm vs. 55.17 ± 25.88 Kgm, t=2.652), AP (30.04 ± 12.59 W vs. 28.02 ± 17.03 W, t=3.618), TW (496.47 ± 257.26 Nm vs. 466.19 ± 225.70 Nm, t=3.227) of 60/s isokinetic muscle strength test and PT (37.22 ± 18.73 Kgm vs. 32.54 ± 15.22 Kgm, t=2.511), AP (39.43 ± 18.02 W vs. 36.14 ± 23.88 W, t=2.183), TW (326.03 ± 159.38 Nm vs. 267.66 ± 156.93 Nm, t=2.073) of 180/s isokinetic muscle strength test in observation group were significantly higher than those in control group (P<0.05). Conclusions Tuina therapy could improve KOA patients' musculus quadriceps fexoris strength, which curative effect were better than oral treatment of Meloxicam.

Objective To investigate the clinical effects differences of two kinds of D2 lymphadenectomy in the treatment of patients with advanced gastric cancer including open operation and laparoscopy operation.Methods 90 patients with advanced gastric cancer were chosen in recent years in our hospital and were randomly divided into both group including group A (45 patients)with D2 lymphadenectomy by open operation and group B (45 patients) with D2 lymphadenectomy by laparoscopy operation;and the clinical indicators in peri-operation period,the dissec-tion number of lymph node,survival rate in 1 year with follow-up and postoperative complication incidence of both groups were compared.Results The clinical indicators in peri-operation period of group B was significantly better than that of group A (t=2.13,2.56,1.98,1.96,1.94,2.05,2.10,all P<0.05).The N2 dissection number of lymph node of group B was significantly fewer than that of group A (t=2.15,P<0.05).The survival rate in 1 year with follow-up of group A and group B were separately 88.89%and 91.11%,there was no significant difference in survival rate in 1 year with follow-up between the two groups(χ2 =1.67,P>0.05).The postoperative complication incidence of group A and group B were separately 20.00% and 8.89%,the postoperative complication incidence of group B was significantly lower than that of group A (χ2 =9.44,P<0.05).Conclusion Compared with D2 lymph-adenectomy by open operation,D2 lymphadenectomy by laparoscopy operation in treatment of patients with advanced gastric cancer can efficiently decrease the degree of surgical trauma,accelerate the process of recovering after opera-tion,and be helpful to reduce the risk of postoperative complications.

Cardiovascular disease, with high morbidity and mortality, has been threatening the health of human beings. Therefore, expecting to find a more effective therapeutic method, a plenty of researchers devote themselves to the study of the cardiovascular disease all the time. Since discovered on the heart, M3 receptor of muscarinic acetylcholine receptor (mAchR, M receptor) became a new starting point of the research of the cardiovascular disease. With more and more investigation, many people found that M3 receptor could protect the heart from kinds of cardiovascular disease, which may make it a new hopeful therapeutic point. So, expecting to give support to the reference and encouragement for the study of disease related to M3 receptor in future, this review expounds M3 receptor on the heart from the main following aspects: the effect on the heart, the influence on the cardiovascular disease and the mechanism of M3 receptor involved.

soda lime). It took significant less time to reach the peak CO concentration with baralyme than with the other two CO2 absorbents. The temperature of top canister went up faster than that of the bottom one with soda lime; whereas with baralyme the temperature of the bottom canister went up faster. Conclusion In a simulated closed circuit the risk of CO poisoning was higher with baralyme than with soda lime. But KOH-free soda lime which still contains NaOH, such as sofnolime, may produce more CO than standard soda lime.