1.A case and pedigree report of hereditary hemorrhagic telangiectasia.
Jiafeng LIN ; Jianqiu CHEN ; Baodong WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(3):209-211
A case of hereditary hemorrhagic telangiectasia was reported. Repeated hemorrhage in nasal and digestive system are main clinical manifestation. Capillary expanded on tongue and finger is the main physical sign. Main clinical manifestation and typical physical signs, combined with family history, can help to establish a diagnosis.
Humans
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Male
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Middle Aged
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Pedigree
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Telangiectasia, Hereditary Hemorrhagic
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genetics
2.Analysis of the expression of the outer membrane protein in carbapenem-resistant Acinetobacter baumannii
Tonghui WANG ; Qingqiang YUAN ; Baodong LING
International Journal of Laboratory Medicine 2015;(16):2295-2296,2300
Objective To Screen the carbapenem-resistant acinetobacter baumannii so as to understand the drug resistance,and to analyze the outer membrane protein expression between the carbapenem-resistant Acinetobacter baumannii and the sensitive strains.Methods The twofold agar dilution method was used to screen the carbapenem-resistant acinetobacter baumannii.PCR technique was used to detect the outer membrane protein-encoding gene carO.SDS-PAGE was used to analyze the expression of outer membrane protein.Results 32 strains were carbapenem-resistant acinetobacter baumannii separated from 1 10 strains,which were 100% sensitive to polymyxin B and were resistant to other drugs with different degrees O.They all carried carO gene.There were different protein bands between the carbapenem-resistant strains and the sensitive strains,in which two protein bands of 50× 10 3 and 22×10 3 were recognized as outer membrane proteins through the analysis of SDS-PAGE.Conclusion Drug resistance of acinetobacter baumannii is serious,and the outer membrane protein expression between the carbapenem-resistant strains and the sensitive strains presents discrepancy.
3.Biocompatiblity of various polypropylene meshes in breast ptosis correction
Zhi WANG ; Wenze WANG ; Hailin ZHANG ; Baodong SUN ; Qun QIAO
Chinese Journal of Medical Aesthetics and Cosmetology 2011;17(5):369-372
Objective Implanting surgical meshes to support breast in periareoler mammaplasty shows an excellent prospect.But shrinking of the mesh and the adhesion formation affected the postoperative shape and palpation of the breast.The aim of this study was to compare biocompatible properties of four different polypropylene meshes (Prolene(R) ; Vypro Ⅱ(R) ; Premilene Mesh (R) and Premilene Mesh LP(R)) and to discuss the inclusion criteria of meshes in this technique.Methods The study was carried out in pigs.Four different polypropylene meshes were implanted subcutaneously in belly.90 days after implantation the mesh samples were explanted,and complications,foreign-body reaction,collagen fiber and elastic fiber formation were observed,as well as ultrastructures for the biocompatible properties.Results The biocompatible properties of each mesh were different.The foreign-body reactions determined the biocompatible properties,and affected the postoperative shape greatly.Conclusions Premilene Mesh LP o as a newer low-weight polypropylene mesh,has superior properties to the others.
4.Effect of hypoxia on cultural hair cells of rat cochlea in vitro
Liping WANG ; Ping WANG ; Bo DU ; Baodong DU
Journal of Jilin University(Medicine Edition) 2006;0(02):-
0.05).IHC loss was found under hypoxia for 2 h to 48 h in a time dependent manner,the cell density in unit area had remarkable difference compared with control(P
5.Expressions of Bcl-2 and Bax protein in cochlea of diabetic rats
Bo DU ; Ping WANG ; Yusheng WANG ; Baodong DU
Journal of Jilin University(Medicine Edition) 2006;0(06):-
Objective To investigate the correlation of cochlear injury and expressions of Bcl-2 and Bax in the cochlea of diabetic rats.Methods Thirty five Wistar rats were randomly divided into diabetic group(n=25) and control group(n=10).Diabetic deafness was induced in rats by intraperitoneal injection of streptozotocin,while the rats in normal group were injected with normal saline.Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in the cochlea after 3 months.Results No significantly morphological change in cochlea of diabetic rats was found.The Bcl-2 levels in stria vascularis in diabetic group and control group were 135.32?21.69 and 45.32?9.08,respectively,there was significant difference(P
6.Effect of Nucha Electroacupuncture on 5-hydroxytryptamine in Cerebrospinal Fluid and Blood of Acute Cerebral Infarction Patients
Baodong LI ; Jing BAI ; Yingguo YANG ; Zhiyong WANG
Chinese Journal of Rehabilitation Theory and Practice 2012;18(7):634-635
Objective To study the effect of nucha electroacupuncture (NEA) on 5-hydroxytryptamine (5-HT) in cerebrospinal fluid (CSF) and blood of acute cerebral infarction (ACI) patients. Methods 72 cases with first ACI were randomly divided into NEA group (n= 38) and control group (n=34). Their 5-HT levels in CSF and blood were determined before and 4 weeks after treatment. Results Before treatment, the 5-HT in the CSF was (0.67±0.13) μmol/L in the NEA group and (0.71±0.11) μmol/L in the control group (P>0.05), while that in the blood was (0.44±0.19) μmol/L in the NEA group and (0.41±0.10) μmol/L in the control group (P>0.05). After treatment, the 5-HT in the CSF was (1.12±0.32) μmol/L in the NEA group and (0.83±0.15) μmol/L in the control group (P<0.05), while that in the blood was (0.87± 0.14) μmol/L in the NEA group and (0.63±0.07) μmol/L in the control group (P<0.05). Conclusion NEA can increase the 5-HT in both CSF and blood after ACI, which may facilitate to reduce post-stroke depression.
7.An investigation and analysis of animal plague in Huairou District of Beijing City between 2009 and 2011
Chao LI ; Jichen ZHOU ; Yang LIU ; Baodong WANG ; Xue YAN
Chinese Journal of Endemiology 2014;33(1):77-79
Objective To investigate the species composition and quantitative dynamic state of rodent and parasitic flea in Huairou District of Beijing City,and to carry out serologic monitoring of animal plague.Methods Rodent density was investigated at different habitats in eight villages and towns between 2009 and 2011.Serum samples from captured rodents were detected by indirect hemagglutination test,and genotypes of collected parasitic fleas were identified.Results A total of 709 rodents which belong to eight different kinds were captured with a rodent density of 2.52% (83/3 300),and Rattus niviventer and Apodemus peninsulae were dominant species,acounted for 46.4% (329/709) and 18.1%(128/709),respctively.Five hundred and sixty-three serum samples of rodents were tested by plague indirect hemagglutination,and all of them were plague antibody negative.Two hundred fleas were checked with a flea infected rate of 29.63% (48/162),and a flea index of 1.23; Paraceras crispus and Leptopsylla pavlovskii were dominant flea species,a total of 30 and 27,respectively.Conclusion Some environment conditions are suitable for rodents survival,even though there is no sign of animal plague epidemic.
8.Protective Effect of N-acetylcysteine on Rifampicin-induced Hepatic Injury in Mice
Yanlin ZHANG ; Baodong YUAN ; Jing XU ; Xiansong WANG
Herald of Medicine 2016;35(7):724-727
Objective To investigate the role of N-acetylcysteine as a protective agent in rifampicin-induced hepatic injury of mice. Methods Thirth-two Kunming mice were randomly divided into four groups(n=8 each).The mice in each group were injected intraperitoneally with 0.9% sodium chloride solution (control), N-acetylcysteine (NAC), combination of rifampicin (R), or NAC and R (NAC+R) once every day.After 14 days, the liver index (LI), alanine aminotransferase (ALT) and aspartate aminotransferase ( AST) activity in serum, and the level of malondialdehyde( MDA) ,superoxide dismutase( SOD) activity in liver tissues were measured respectively.Hepatic tissue morphology was observed under light microscope. Results Macroscopic analysis revealed that rifampicin led to severe liver tissue injury,including a wide range of hepatocellular vascular congestion,fatty change and local necrosis, whereas the administrationof NAC produced a significant reduction of rifampicin-induced hepatotoxicity .LI,ALT and AST activities in R or NAC+R group were significantly elevated as compared with the control group(P<0.01) .LI, activities of ALT and AST in serum,and MDA levels in liver tissues in NAC+R group were significantly lower than those in R group ( P<0.01) ,but the SOD activity in NAC+R group was increased significantly in comparison with R group (P<0.01). Conclusion Rifampicin was able to cause severe hepatic injury.Pre-administration of NAC reduced the side-effect induced by the treatment with the rifampicin.
9.Relationship between thyrotropin receptor gene mutation and toxic multinodular goiter
Fengjun WANG ; Baodong GAI ; Zhongtian JIN ; Deheng ZHANG
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
DNA was extracted from thyroid nodular tissue in 16 patients with toxic multinodular goitor (TMG). After PCR, the product of PCR was sequenced, and 3 cases with point mutation (A1964T) and 2 cases with insertion mutation (a G was inserted after nucleotide 1928) in TSH receptor gene were found. It is postulated that these mutations may play an important role in the pathogenesis of TMG.
10.Application of ~(125)I seeds brachytherapy in advanced head and neck malignant tumor combined with surgery
Bo DU ; Yusheng WANG ; Yan ZHANG ; Baodong DU
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(07):-
OBJECTIVE To evaluate the effectiveness of ~(125)I seeds brachytherapy in advanced head and neck malignant tumor.METHODS Twelve patients with post-radiotherapy recurred laryngeal cancer with bilateral cervical lymph node metastasis, tongue cancer with bilateral cervical lymph node metastasis or recurred maxillary sinus cancer and so on were operated radically,and ~(125)I seeds were implanted in wound area during operation.RESULTS With a follow-up of 24 to 32 months,no tumor recurred in 11 patients,and only one patient with tongue cancer recurred at 6 month after operation.No local infection, acute radioactive lesion,vessel broken or seeds migration happened.CONCLUSION The ~(125)I brachytherapy can be used as one of the comprehensive treatment method in advanced head and neck malignant tumors,especially in the recurred tumors after operation or radiotherapy or in the cancer which can not be radically removed.It's also an effective method to improve local control in the subset of patients with doubtful positive margins.