AIM: To establish a method for determing GC fingerprint of the chemical constituents in Bulbus lilii. METHODS: GC/MS were used to optimize the fingerprint determination, and to identify the main peaks in the GC fingerprint. RESULTS: A preferable determination for GC fingerprint of the chemical constituents in Bulbus lilii.was established. CONCLUSION: A general acquaintance of the chemical constituents in Bulbus lilii.can be obtained by using the preferable GC fingerprint determination method,which is useful for quality evaluation of the crud drug of Bulbus lilii.

AIM: To compare the chemical compositions in the expressed oil from euphorbia before and after processing.METHODS: GC-MS was used to compare the chemical compositions in the expressed oil before and after processed euphorbia.RESULTS : Tirty-four chemical compounds were detected in the expressed oil of the raw and 30 of the processed.CONCLUSION: Processing has the major effect on the chemical compositions in the expressed oil of euphorbia.

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AIM:To compare the chemical compositions in the expressed oil from euphorbia before and after processing.METHODS:GC-MS was used to compare the chemical compositions in the expressed oil before and after processed euphorbia.RESULTS:Tirty-four chemical compounds were detected in the expressed oil of the raw and 30 of the processed.CONCLUSION:Processing has the major effect on the chemical compositions in the expressed oil of euphorbia.

AIM: To compare the difference between medicinal and edible lilium brownii. METHODS: GC method was used for the determination of stigmasterol (the reference standard) in medicinal and edible Lilium brownii. And pharmacology experiment on stigmasterol was carried out. RESULTS: Stigmasterol had the antiinflammatory and anti-coughing effect. It represented the main effect of Lilium brownii. It was reasonable to be the reference standard. There were obvious differences in the stigmasterol content of medicinal and edible Lilium brownii. The stigmasterol content of medicinal Lilium brownii was much higher than that of the edible. CONCLUSION: Edible Lilium brownii can not be applied to clinical.

Objective To develop a LC-MS/MS method for the determination of inosine in human plasma and to apply it in the isoprinosine pharmacokinetic researches .Methods With adefovir as the internal standard ,methanol -10 mmol/L ammonium ace-tate(15 :85 ,v/v) was adopted as the mobile phase .The chromatographic column was the Agilent SB-C18 column(5 μm ,150 mm × 4 .6 mm I .D .) .Electrospray ionization(ESI) source was applied and the detection was conducted by the multiple-reaction monito-ring(MRM) mode .The ion reactions for the quantitative analysis were m/z 135 .0-267 .3(inosine) and m/z 134 .1-272 .0(adefo-vir) .Results The linear range of plasma inosine was 10-3 000 ng/mL .The lower detection limit was 10 ng/mL .The inter-and in-tra-day precisions(RSD) were less than 5% .The average recovery rate was above 90% without the matrix effect .Conclusion This method has the strong specificity ,convenience in treating the sample and high sensitivity ,and is suitable for the inosine pharmacokinetic re-searches .

Objective To optimize the extraction process and to establish a method for the content determination of the polysaccharides in Ophiopogonjaponicus.Methods With extracts rate of polysaccharide as the index,an orthogonal de- sign test was adopted to optimize the extraction process.And phenol-sulphuric acid colorimetric method was used to de- termine the content of polysaccharides in Ophiopogonjaponicus.Results The optimum extract conditions were as follows: refluxing twice with eight times amount of water,thirty minutes each time,and precipitation with 80 % alcohol.A good linearity of polysaccharides was in range of 0.02032～0.10008 mg(r=0.9998),and the average recovery rate was 102.41%,RSD was 1.78 %.Conclusion The polysaccharide can be fully extracted under these conditions.

Objective To study the phase transformations and chemical composition changes of Pyritum Preparata (PP) influenced by different processing. Methods To analyse the changes of microstructure, morphology, mineral phase, and chemical compositions in different PP by ore microscope, electron probe microanalyzer technique. Results Greater changes have taken place in chemical compositions of PP after calcination: pyrite (FeS_2) changed into pyrrhotite (Fe_(1-x)S), even changed into magnetite (Fe_3O_4); and chalcopyrite generated mutation to be low-copper chalcopyrite, the contents of Cu reduced, the rate of Cu/Fe changed. At the same time, in the pyrrhotite around the mutative chalcopyrite, the contents of Cu increased. That is to say, the Cu got into pyrrhotite and generated solid-solution with high dispersion, and then ore became loose and porous. In the hard-burned spot of calcined quenched samples by vinegar, the contents of Pb and S are far lower than non-burned spot, the minimum estimate of Pb is less than 1/10 of crude ore. Conclusion There is a co-relativity between phase transformations and dissolution of chemical compositions before and after calcining PP.

Objective To observe the influence of storage time and condition on the chemical components in the volatile oil of Fructus Evodia by GC-MS.Methods The volatile oil was extracted from Fructus Evodia by steam distillation and the amount of each components in the volatile oil was determined by area normalization method and the components were identified by GC-MS.Results One hundred and sixteen components were separated and identified,which accounted for more than 90% in the total constitutes of the volatile oil.The content of volatile oil in Fructus Evodia was influenced by the storage time and condition.Conclusions The method is reliable and has a good repeatability,and can be applied to analyze volatile components in Fructus Evodia.This will provide a certain scientific basis for the storage and clinical application of Fructus Evodia.

Objective To research the pharmacodynamics of lilium brownii Polysaccharide.Methods Five tests,including hypoxia tolerance test,serum content determination of SOD and MDA in mice,carbon clearance and weight determination of immune organ,content determination of serum hemolysin,and delayed-type hypersensitivity test were carried out to evaluate the main pharmacological indexes of lilium brownii Polysaccharide.Results Lilium brownii Polysaccharide can increase the hypoxia tolerance,anti-oxidation action and immuno-enhancing activity in mice.Conclusion Lilium browni Polysaccharide is one of the main active constituents of lilium brownii.