Objective To establish a HPLC method for the determination of allantoin in Rhizoma Dioscoreae from different producing areas. Methods The RP- phase HPLC is applied. Separation and determination of allantoin was achieved by using an Allttima ODS C18 column(250 mm? 4.6 mm, 5? m) and a mobile phase of methanol- water (1 ∶ 39, V/V). The flow- rate was 0.8 mL/min, temperature at 30 ℃ and detection wavelength at 224 nm. Results A good linearity of allantoin was in the range of 0.24 ? g～ 2.40 ? g, r=0.9992; the average recovery rate was 103.1 % , RSD =2.0 % (n=6). Conclusion Rhizoma Dioscoreae from Henan Province is of the best quality and Rhizoma Dioscoreae from other provinces are only qualified for food. 

AIM: To establish a method for determing GC fingerprint of the chemical constituents in Bulbus lilii. METHODS: GC/MS were used to optimize the fingerprint determination, and to identify the main peaks in the GC fingerprint. RESULTS: A preferable determination for GC fingerprint of the chemical constituents in Bulbus lilii.was established. CONCLUSION: A general acquaintance of the chemical constituents in Bulbus lilii.can be obtained by using the preferable GC fingerprint determination method,which is useful for quality evaluation of the crud drug of Bulbus lilii.

Quality standard research of pieces of Chinese crude drugs is the important content of research of Chinese medicine processing, and it is also the key point of the whole standardization system of Chinese medicine. Although we have set up a preliminary quality standard system of pieces of Chinese crude drugs, it still under a low level. During the research of quality standard of pieces of Chinese crude drugs, we still face some problems, such as how to set up a more comprehensive, scientific, and reasonable quality standard. In this paper, we proposed some advices that we should pay more attention to traditional processing theories, processing mechanisms and effective substances during research of quality standard research of pieces of Chinese crude drugs.

AIM: To compare the chemical compositions in the expressed oil from euphorbia before and after processing.METHODS: GC-MS was used to compare the chemical compositions in the expressed oil before and after processed euphorbia.RESULTS : Tirty-four chemical compounds were detected in the expressed oil of the raw and 30 of the processed.CONCLUSION: Processing has the major effect on the chemical compositions in the expressed oil of euphorbia.

Objective To establish a HPLC method for the simultaneous determination of chlorogenic acid,liquiritigenin,cinnamic acid,and monoammonium glycyrrhetate in Tong Saimai Pellets.Methods A Zorbax 80A Extend-C18 column was used.The mobile phase consisted of acetonitrile and 0.1% phosphoric acid,and was used for gradient elution.Chlorogenic acid,liquiritigenin,cinnamic acid and monoammonium glycyrrhetate were determined by dual wavelength,?s=276nm,?R=590nm.Results The linear range of chlorogenic acid was 0.0505~1.6160 ?g,and r=0.9999;the average recovery was 98.17%,and sR=4.28%(N=6).The linear range of liquiritigenin was 0.0252~0.8064 ?g,and r=0.9999;the average recovery was 93.76%,and sR=1.94%(N=6).The linear range of cinnamic acid was 0.0111~0.3552?g,and r=0.9999;the average recovery was 98.25%,and sR =2.72%(N=6).The linear range of monoammonium glycyrrhetate was 0.3300~10.5600?g,and r=0.9999;the average recovery was 102.6%,and sR=1.93%(N=6).Conclusion This method is convenient,rapid and accurate,and it can be used for quality control of the production of Tong Saimai Pellets.

AIM:To compare the chemical compositions in the expressed oil from euphorbia before and after processing.METHODS:GC-MS was used to compare the chemical compositions in the expressed oil before and after processed euphorbia.RESULTS:Tirty-four chemical compounds were detected in the expressed oil of the raw and 30 of the processed.CONCLUSION:Processing has the major effect on the chemical compositions in the expressed oil of euphorbia.

Objective To optimize the technology for processing Fructus Corni steamed with alcohol. Methods With the yield of polysaccharide from Fructus Cornias as the indicators,three factors(of wine volume,moistening time, steaming time)were optimized by orthogonal test . Results The optimal processing technology for processing Fructus Corni is as follows: the wine volume for 25 %,moistening for 2 h, steaming for 4 h. Conclusion The optimal processing technology for processing Fructus Corni is reasonable.

Objective To compare the immunoregulatory role of polysaccharides from Rhizoma Dioscoreae and Rhizoma Dioscoreae Preparata in mice with immunosuppression. Methods Carbon clearance test and hemolysin test were used to evaluate the non-specific and specific immunoregulatory roles of polysaccharides from Rhizoma Dioscoreae and Rhizoma Dioscoreae Preparata by different extraction methods..Result The polysaccharides extracted with cold water from Rhizoma Dioscoreae Preparata had a stronger effect than Rhizoma Dioscoreae on increasing the index of K and enhancing phagocytic function of mononuclear-macrophage and hemolysin level. Conclusion The polysaccharides extracted with cold water from Rhizoma Dioscoreae Preparata have a stronger effect in improving the cellular and humoral immune function than Rhizoma Dioscoreae, which accords with the clinical application and will lay a foundation for further explaining the processing mechanism of Rhizoma Dioscoreae Preparata.

AIM: To compare the difference between medicinal and edible lilium brownii. METHODS: GC method was used for the determination of stigmasterol (the reference standard) in medicinal and edible Lilium brownii. And pharmacology experiment on stigmasterol was carried out. RESULTS: Stigmasterol had the antiinflammatory and anti-coughing effect. It represented the main effect of Lilium brownii. It was reasonable to be the reference standard. There were obvious differences in the stigmasterol content of medicinal and edible Lilium brownii. The stigmasterol content of medicinal Lilium brownii was much higher than that of the edible. CONCLUSION: Edible Lilium brownii can not be applied to clinical.