BACKGROUND:At home and abroad, a large number of studies have demonstrated that repetitive transcranial magnetic stimulation can evoke changes in cortical excitability that can be sustained beyond the time of stimulation, which provides a new research direction for application of magnetic stimulation in rehabilitation treatment for cerebral infarction. However, its long-term clinical efficacy and safety deserve further investigation. OBJECTIVE: To observe the influence of repetitive transcranial magnetic stimulation on nerve regeneration microenvironment in the infarcted cerebral area and rat neurological functional recovery after cerebral infarction. METHODS:The rats were randomly divided into model group, sham stimulation group and repetitive transcranial magnetic stimulation group (80%, 100% and 120% motor threshold subgroups). Right middle cerebral artery occlusion models were established in rats by the intraluminal suture method. At 24 hours after middle cerebral artery occlusion, 20 Hz repetitive transcranial magnetic stimulation was performed in each repetitive transcranial magnetic stimulation subgroup at the designated motor thresholds. Rats in the sham stimulation group underwent sham stimulation, while the model group rats were not given any treatment. RESULTS AND CONCLUSION: Seven days after middle cerebral artery occlusion, cerebral infarction volume in the repetitive transcranial magnetic stimulation group was significantly less than that in the model and sham stimulation groups (P < 0.05). RT-PCR and Western blot assays showed that at 72 hours after middle cerebral artery occlusion, aquaporin 4/9 mRNA and protein expression in the repetitive transcranial magnetic stimulation group was significantly increased than in the model group (P < 0.05). Compared with 1 day after middle cerebral artery occlusion, neurological deficit scores at 15 days after repetitive transcranial magnetic stimulation was significantly improved (P < 0.05). Immunohistochemical detection results showed that glial fibrilary acidic protein expression in the ischemic penumbra in each repetitive transcranial magnetic stimulation subgroup was significantly lower than that in the model group (P < 0.05). The results reveal that repetitive transcranial magnetic stimulation can aleviate rat neurological deficits and improve nerve regeneration microenvironment by inducing cerebral ischemic tolerance, reducing nerve cel apoptosis and decreasing aquaporin 4/9 mRNA and protein expression.

Objective　To investigate the effect of rapid fluid replacement on hemorheology in delayed resuscitation after burn. Methods　A total of 32 dogs inflicted with 40%TBSA full thickness scalding were randomly divided into 4 groups: scald control group(C group), delayed Gelofusion even replacement group (GE group), rapid fluid replacement group (GR group), and delayed plasma rapid fluid replacement group (PR group). The femoral arterial pressure, viscosity of blood and plasma, packed cell volume and aggregation of RBC were detected at the intervals of before and 2, 6, 8, 12, 24, 36 and 48 hours after scalding. Results　The viscosity of blood markedly increased at 2 hours after scalding, and the hemorheology parameters decreased after fluid resuscitation. The hemorheologic parameters were obviously lower in GR group than in GE group at 2 hours after rapid resuscitation, the viscosity of blood and RBC aggregation in GR group were obviously lower than those in PR group. Conclusion　Under the condition of delayed resuscitation after burn, rapid fluid replacement can quickly decreased the state of blood high viscosity and may play a role in improving microcirculation and treating burn shock.

OBJECTIVETo investigate the influence of N-acetyl-L-cysteine (NAC) on pulmonary injury and oxygen stress caused by smoke inhalation injury.

METHODSRats inflicted with smoke inhalation injury were employed as the model. WBC in BALF (bronchoalveolar lavage fluid), MPO (myeloperoxidase) activity, hydrogen peroxide (H(2)O(2)) content, GSH (glutathione) content and total antioxidant (TAO) capacity in pulmonary tissue were determined.

RESULTSPostinjury WBC in BALF, MPO activity in pulmonary tissue and H(2)O(2) content decreased obviously after NAC treatment. But the pulmonary tissue contents of GSH and ATO increased evidently after the treatment with NAC.

CONCLUSIONNAC treatment could ameliorate pulmonary oxygen stress after smoke inhalation injury. AS a result, the pulmonary antioxidant capacity was improved.

Acetylcysteine ; pharmacology ; Animals ; Bronchoalveolar Lavage Fluid ; Disease Models, Animal ; Free Radical Scavengers ; pharmacology ; Glutathione ; metabolism ; Hydrogen Peroxide ; metabolism ; Leukocytes ; drug effects ; Oxidative Stress ; drug effects ; Peroxidase ; metabolism ; Rats ; Rats, Wistar ; Smoke Inhalation Injury ; enzymology ; metabolism ; pathology

Objective:To investigate the serum metabolites and their metabolic characteristics of patients with optic neuritis.Methods:Case-control study. From January 2021 to January 2022, 9 serum specimens of diagnosed patients with optic neuritis were collected in Department of Neurology from Beijing Tongren Hospital and 9 healthy subjects as the control. Among them, there were 5 females and 4 males in the optic neuritis group, aged (35.8±12.9) years; there were 5 females and 4 males in the healthy control group, aged (32.6±8.6) years. Liquid chromatography-mass spectrometry was used to detect metabolites in serum of healthy control and patients with optic neuritis. The principal component analysis (PCA) and orthogonal partial least-squares discriminination analysis (OPLS-DA) were used to analyze the differential metabolites . The variable importance projection value of OPLS-DA model and the P value of t-test was applied to find the different metabolites. Results:Thirty-seven metabolites were finally identified from serum samples. Four metabolites with variable important in projection (VIP) values larger than 1 and P values less than 0.05 were teased out, three metabolites, LysoPC (P-16∶0), LysoPC (16∶0), LysoPC (P-18∶0) belonge to phospholipid and one metabolite was L-Threonine, they were all down-regulated. The area under curve were 0.951, 0.889, 0.963 and 0.944, respectively. Conclusion:Based on metabonomic analysis, some metabolites in serum have changed, which can provide basis for biomarkers screening of optic neuritis.