Objective To detect the effects of laminin and type Ⅳ collagen on the attachment and proliferation of Schwann cells Method The plated were covered by laminin and collagen Ⅳ, the control groups were added antibody of laninin and collagen Ⅳ, collagen Ⅰ and polylysine The same concentration of Schwann cells were cultured in each groups plates The rates of cells attachment of each group were determined after 2,6,24 hours After 72 hours 3*"H TdR were mixed into culture matrix fluid and the cpm were mensurate Results The attachment rates of Laminin and collagen Ⅳ groups were higher than that of the control groups and the amount of mixed 3*"H TdR were also higher than that of control groups Conclusion Laminin and collagen Ⅳ promote the attachment and proliferation of Schwann cells

BACKGROUND: Schwann cells play an important role in regeneration of peripheral nerves and the extracellular matrix(ECM) is also important to growth of Schwann cells.OBJECTIVE: To explore the effects of laminin and type Ⅳ collagen on the adhesion and proliferation of Schwann cells.DESIGN: A controlled trial in groups with Schwann cell as subject.SETTING: Plastic Surgery Laboratory of the Fourth Military Medical University of Chinese PLA.MATERIALS: The trial was conducted in the Plastic Surgery Laboratory of Xijing Hospital Affiliated to the Fourth Military Medical University of Chinese PLA from January through April in 1995. The Schwann cells were extracted from newborn rabbits.METHODS: The culture plates were coated with laminin and type Ⅳ collagen. While in the control group, the antibody of laminin and collagen Ⅳ,collagen Ⅰ and polylysine were added respectively. The Schwann cells of the same concentration were cultured in plates of each group. The cells attachment rate in each group ,was determined after 2, 6, 24 hours respectively. After 72 hours 3H-TdR were incorporated into culture matrix fluid and double channel liquid scintillation counter was used for measurement of radiactivity count per minute.MAIN OUTCOME MEASURES: ① Attachment rate of Schwann cell. ②3H-TdR counting.RESULTS: The attachment rates in laminin and collagen Ⅳ groups (66%and 59% ) were higher than those of the type Ⅰ collagen and polylysine groups(45% and 43% ) after 2-hour culturing. Those in laminin antibody and collagen Ⅳ antibody groups were lower. The incorporation value were (10.0±2.7)×103, (1.3±0.3)×103, (10.4±2.4)×103, (1.4±0.5) ×103, (5.8±2.7)×103, (3.3±1.0)×103 per minute respectively in laminin, laminin antibody, collagen Ⅳ, collagen Ⅳ antibody, collagen Ⅰ and polylysine groups. The incorporation values in laminin and collagen Ⅳgroups were higher than controls.CONCLUSION: Laminin and type Ⅳ collagen promote the attachment and proliferation of Schwann cells in vitro. This study provides experimental basis for applying the action of Schwann cell to the nerve tissue engineering.

BACKGROUNDTo explore the clinical significance of detection of T helper cell (Th1 and Th2) in patients with lung cancer and to provide a foundation for immunological treatment.

METHODSRIA and ELISA were used to detect the level of serum IL-2, IL-4, IL-6, IL-8 and TNF-a in 86 patients with lung cancer, 59 patients with benign pulmonary diseases and 45 healthy people. IL-2 and TNF-a were used to represent cytokines of Th1 type, and IL-4, IL-6 and IL-8 to represent cytokines of Th2 type.

RESULTSThe level of IL-2 [(24.6±12.0) μg/L]in cancer group was significantly lower than that in benign group [(71.1±25.4) μg/L] ( t =3.82, P < 0.01) and normal group [(69.3±19.5) μg/L]( t=2.76, P < 0.01), the level of IL-6 in cancer group [(0.13±0.04) μg/L] was significantly lower than that in normal group [(0.23±0.05) μg/L]( t= 3.39 , P < 0.01), but the levels of IL-4 [(254.2±78.0) μg/L], IL-8 [(0.49±0.16) μg/L], and TNF-a [( 2.76 ±1.12) μg/L] in cancer group were significantly higher than those in benign group [(63.6±18.6) μg/L, ( 0.36 ±0.18) μg/L, (0.96±0.20) μg/L respectively] and those in normal group [(60.9±19.6) μg/L, ( 0.35 ±0.07) μg/L, (0.93±0.19) μg/L respectively] ( t =4.10, 4.89, 3.76 respectively, all P < 0.01). No significant difference of IL-2, IL-4, IL-8 and TNF-a level was observed between benign group and normal group (all P > 0.05). The level of IL-6 in cancer group was similar to that in benign group [(0.15±0.04) μg/L] ( P > 0.05 ). The level of IL-6 in benign group was significantly lower than that in normal group [(0.23±0.05) μg/L] ( P > 0.05 ). There was no significant difference in these cytokines among lung cancer patients with different histological types and in different TNM stages.

CONCLUSIONST helper cell cytokines are out of balance in patients with lung cancer, and this may play a certain role in the pathogenesis of lung cancer. Correcting this immune malfunction may become an important method in lung cancer therapy.

BACKGROUNDMany researches indicate that endocrine imbalance plays an important role in the oncogenesis and development of malignant tumor. The aim of this study is to investigate the clinical significance of serum sexual hormones' level in male patients with lung cancer.

METHODSRIA and IRMA were used to detect the levels of serum estrodiol (E₂ ), testosterone (T), prolactin (PRL), growth hormone (GH), luteinizing hormone (LH), follicle-stimulating hormone(FSH) and thyroid-stimulating hormone (TSH) in 62 lung cancer patients, 30 benign pulmonary disease patients and 30 healthy men.

RESULTSThe level of T in male patients with lung cancer was obviously lower than that in healthy men (t=0.348, P < 0.01 ); the levels of E₂, PRL and FSH remarkably increased in male patients with lung cancer compared with healthy men (t=0.362, P < 0.01; t=2.913, P < 0.05; t=2.739, P < 0.01); the level of T in male patients with lung cancer was obviously lower than that in patients with benign pulmonary diseases (t= 3.903 , P < 0.05 ); the levels of T in squamous cell carcinoma group and adenocarcinoma group decreased significantly compared with small cell lung cancer group (t=0.358, P < 0.01; t=3.902, P < 0.05). The levels of FSH and TSH in squamous cell carcinoma group were significantly higher than those in adenocarcinoma group (t= 3.918, P < 0.01; t=2.912, P < 0.05). The level of T in lymph node metastasis group decreased obviously compared with non-lymph node metastasis group (t=3.914, P < 0.01), but the other hormones were at the same level in different pathological types of lung cancer.

CONCLUSIONSThere are some disturbance of serum sexual hormones' level in lung cancer patients. The detection of serum E₂ , T, PRL and FSH may have some value to lung cancer diagnosis and can be an index to predict the state of lung cancer.