1.Cilinical analysis of invasive fungal infection in patients with newly diagnosed acute leukemia during their induction therapy.
Jin LU ; Xijin LU ; Li BAO ; Xiaohui ZHANG ; Xiaojun HUANG
Chinese Journal of Practical Internal Medicine 2001;0(03):-
Objective To analyze the incidence of invasive fungal infection and the risk factor in primary acute leukemia patients.Methods Retrospectively analysis of clinical data from Jan 2007 to Jun 2008 about 80 newly diagnosed patients with acute leukemia primary treated in Peking University People's hospital.Results The incidence of invasive fungal infection is 13.8%,elderly patients、higer WBC counts and longer antibiotics treatment are the risk factor for the fungal infection.Conclusion There are high incidence of invasive fungal infection in induction therapy for primary acute leukemia,the prophylaxis therapy should be consider in high risk patients.
2.The survey about health knowledge condition in HBV-positive pregnant women
Meijuan BAO ; Yan LU ; Xiaoling WANG ; Juan ZHANG ; Lin ZHANG
Chinese Journal of Practical Nursing 2009;25(23):34-36
Objective To investigate the HBV- positive pregnant women about the knowledge of the route of hepatitis B transmission, hepatitis B hazards, health care during pregnancy, announcements of child- care to explore targeted and effective health education model with self- designed question-naires. Methods Nurses in obstetrics department carried out this questionnaire investigation in the outpa-tients of HBV- positive pregnant women, after that the results underwent statistic analysis. Results The overall knowledge of HBV- positive pregnant women in announcements of child- care, health care during pregnancy and hepatitis B hazards was not very well, and the announcements of child- care was the lowest, followed by health care during pregnancy and hepatitis B hazards. The route of hepatitis B transmission was better, but still not satisfactory. The education should highlight these areas. Well- educated women were better than those who lack education. The age between 20 to 30 years old was better. Conclusions The HBV-positive pregnant women is lack of heath knowledge, so we should gave health intervention in a multi- level, multi- stage standardized and a variety of ways.
3.Progress in the treatment of diabetic wound healing via stem cells transplant.
Qing-xin CAI ; Lu WANG ; Yan ZHANG ; Bao-feng YANG
Acta Pharmaceutica Sinica 2015;50(1):1-6
The morbidity of diabetes has been increasing rapidly in recent years. Delayed wound healing has become a common complication in diabetes, which seriously affects the orthobiosis of patients. Exploring and finding the molecular mechanisms of diabetic wound healing and the effective therapies to promote wound healing have important clinical significances. Stem cells transplant has become a research hotspot in accelerating diabetic wound healing. This article reviewed the present approaches concerning stem cells transplant in diabetic wound healing both at domestic and abroad, and looked forward the clinical therapy of stem cells on diabetic wound healing.
Diabetes Mellitus
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therapy
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Humans
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Stem Cell Transplantation
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Stem Cells
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Wound Healing
4.Endoplasmic reticulum stress-mediated high-fat diet, palmitic acid-induced insulin resistance in skeletal muscle and interventional effects of fenofibrate
Yingying BAO ; Yunxia LU ; Guanjun CHEN ; Jingjing CHENG ; Qiu ZHANG
Chinese Pharmacological Bulletin 2014;(11):1557-1563
Aim To investigate endoplasmic reticulum stress ( ERS)-mediated high-fat diet and palmitic acid-induced insulin resistance ( IR) in skeletal muscle and interventional effects of fenofibrate both in vivo and in vitro tests. Methods Female SD rats were randomly subjected to a standard control diet ( SCD) or high-fat diet ( HFD) for 20 weeks, then the HFD group was di-vided into high-fat-diet group and high-fat-diet group treated with fenofibrate ( HFD +FF, 30 mg · kg-1 · d-1 ) for another 8 weeks. The changes of IR and ex-pression of peroxisome proliferator-activated receptor α( PPARα) , glucose regulated protein 78 ( GRP78 ) and transcription factors GADD153 ( CHOP ) were as-sessed respectively. C2C12 myotubes were divided into normal control group ( NC ) , model group ( palmitic acid, PA) , postive control drug group ( tunicamycin, TM) and treatment group ( fenofibric acid, FA+PA) , the expressions of GRP78 and CHOP were assessed re-spectively. Insulin-stimulated phosphorylation of Akt was also analyzed to detect changes of insulin sensitivi-ty in C2 C12 . Results The high-fat diet induced obvi-ous IR and upregulated ERS markers GRP78 and CHOP in skeletal muscle of rats, and these responses were attenuated by treatment with fenofibrate. Incuba-tion of myotubes with palmitic acid or tunicamycin sig-nificantly increased expression of ERS markers GRP78 and CHOP. Meanwhile, insulin-stimulated phosphoryl-ation of Akt was inhibited obviously. Pre-incubation with FA markedly inverted PA-induced ERS and insu-lin-stimulated phosphorylation of Akt. Conclusion Fenofibrate ( fenofibric acid) has obvious effects of IR on skeletal muscle tissues and cells, which may be re-lated with reduced expression of GRP78 and CHOP in ERS.
5.RU486 reverses dexamethasone inhibition of interleukin—2 prodnction and gene expression in lymphocytes of rat spleen
Fa-Quan LIANG ; Bao-Zhang LU ; Pei-Shen TANG ;
Chinese Journal of Immunology 1985;0(05):-
By using ~3H—TdR incorporation and CTLL—2 cell line,we observed effects of RU486,anantagonist of type Ⅱ glucocorticoid receptor,on dexamethasone inhibition of lymphocyte prolifer-ation and interleukin—2(IL—2)production in rat spleen.It was shown that RU486 obviouslyantagonized the inhibitory actions of dexamethasone on lymphocyte proliferation and IL—2 pro-duction.By means of dot hybridization and Northern blot analysis,effects of dexamethasone andRU486 on IL—2 gene expression were investigated in lymphocytes of rat spleen.The datademonstrated that dexamethasone markedly decreased IL—2 mRNA production,RU486 alonedidn't affect IL-2 mRNA levels,but obviously reversed dexamethasone-mediated downregala-tion of IL—2 mRNA production in ConA—activated lymphocytes.These results suggest thatthe above effects of dexamethasone may be mediated by glucocorticoid receptor in lymphocytesof rat spleen.
6.rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis in Zhejiang Province
Yingyan LU ; Wei WANG ; Yaping BAO ; Weimin ZHANG ; Fujian LI
Chinese Journal of Clinical Infectious Diseases 2013;(1):27-30
Objective To characterize rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis (M.tuberculosis) in Zhejiang Province.Methods A total of 188 clinical isolates of M.tuberculosis from 188 patients with pulmonary tuberculosis in Tongde Hospital of Zhejiang Province and Integrated Chinese and Western Medicine Hospital of Zhejiang Province were collected.Conventional drug resistance analysis was performed and the mutation of rpoB gene was detected by PCR-based DNA sequencing.The association between gene mutations in rifampin-resistance determining region of M.tuberculosis and clinical resistance was analyzed.Results Fifty-seven out of 188 isolates (30.3%) were drug-resistant strains,including 18 isolates (9.6%) with single-resistance to rifampin,28 isolates (14.9%) with single-resistance to other anti-tuberculosis drugs (10 to isoniazid,12 to streptomycin and 6 to ethambutol),and 11 isolates (5.9%) with multi-drug-resistance (rifampin plus one or more drugs of isoniazid,streptomycin and ethambutol).Among 29 rifampin-resistant strains,rpoB gene mutation existed in 27 strains (93.1%),and the most frequently mutated sites were codons 526 (55.6%,16/27),513 (22.2%,5/27),531 (14.8 %,4/27)) and 529 (7.4%,2/27).Among 28 strains which were resistant to other anti-tuberculosis drugs,rpoB mutations existed in 4 strains (14.3%),and the mutated sites were codons 526 (2 strains) and 513 (2 strains).All 13 sensitive isolates had no mutation in rpoB gene.Conclusion Rifampin resistance in M.tuberculosis is closely correlated with rpoB gene mutations in Zhejiang province,and the most frequent sites of mutation are at codons 526 and 513.
8.Bortezomib-based combination therapy for newly diagnosed multiple myeloma
Li BAO ; Xijing LU ; Xiaohui ZHANG ; Yan ZHANG ; Honghu ZHU ; Xiaojun HUANG
Chinese Journal of Internal Medicine 2008;47(2):107-110
Objective To study retrospectively the response and side effects in two groups of patients with untreated multiple myeloma receiving bortezomib-based regimen(VD/VT)and vincristine combined with adriamycin and dexamethasone(VAD).Methods Eighteen patients were enrolled in a group of VD or VT,receving bortezomib 1.0 mh/m2 or 1.3 mg/m2 on days 1,4,8 and 11,along with dexamethasone 20-40 mg on days 1-4(12 cases);or thalidomide 100 mg/d continuously(6 cases). Twenty-four patients treated with VAD entered into a control group,receiving vincristine 0.4 mg/d on days 1- 4,adriamycin 9 mg·m-2·d-1 on days 1-4 and dexamethasone on days 1-4,9-12,17-20,with 28 days as a cycle.Results After bortezomib-based combinations,16 of with 18 patients(88.9%)achieved at least a partial response,including complete response and near complete response in 7 patients(38.9%).Side effects in the VD/VT group were predictable and manageable;they were mainly haematologic, gastrointestinal,and peripheral neuropathic and were more evident during early cycles.The main side reactions in the VAD group were infections.loss of hair and phlebitis.Conclusion Bortezomib-based combinations therapy is an effective and safe induction regimen for newly diagnosed multipli myeloma patients and appears significantly superior to VAD,yielding high response rates even in patients with poor prognostic features.
9.Study on suitable harvest time of Dendrobium officinale in Yunnan province.
Shan-bao ZHANG ; Ke-jun ZHOU ; Zhen ZHANG ; Rui-rui LU ; Xian LI ; Xiao-hua LI
China Journal of Chinese Materia Medica 2015;40(18):3549-3552
In order to determine the suitable harvest time of Dendrobium officinale from different regions in Yunnan province, the drying rate, mannose and glucose peak area ratio, extract, contents of polysaccharide and mannose of D. officinale samples collected from six producing areas in Ynnnan province were determined. The results indicate that drying rate and the contents of polysaccharide and mannose arrived the peak from January to April, extract reached a higher content from September to December, and mannose and glucose peak area ratio from October to February of the coming met the requirment of the Chinese Pharmacopoeia. Hence, the suitable harvesting time of D. officinale in Yunnan province is from December to February of the coming year,according to the experimental results and the request of the Chinese Pharmacopoeia.
China
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Dendrobium
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chemistry
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growth & development
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metabolism
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Glucose
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analysis
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metabolism
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Mannose
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analysis
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metabolism
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Time Factors
10.Correlation Analysis between Serum Complement C3,C4 Levels and HDL-C in Patients with Coronary Atherosclerotic Heart Disease
Ruiting LIN ; Bo ZHANG ; Canxia HUANG ; Runlu SUN ; Weifeng LU ; Jinlan BAO ; Yuling ZHANG
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(1):72-77
Objective]The aim of this study was to investigate the level of serum C3,C4 and HDL-C in patients with coronary heart disease and the correlation between C3,C4 and HDL-C in patients with coronary heart disease.[Methods]We collected 251 cases of patient diagnosed with coronary artery disease by coronary angiography in Sun Yat-sen Memorial Hospital ,Sun Yat-Sen University from 2015-12 to 2016-07 and collected over our Boji Medical Center healthy people in 214 cases. These patients were divided into acute coronary syndrome group with 180 cases and stable coronary heart disease group with 71 cases. Each test results was adopted from clinical laboratory of Sun Yat-Sen Memorial Hospital ,Sun Yat-Sen University.[Results]Compared with the healthy control ,the difference of serum C3,C4 and HDL-C from acute coronary syndrome group and stable coronary heart disease group,was statistically significant(P<0.05). However,in patients with acute coronary syndrome and stable coronary artery disease groups,there was no significant correlation(P > 0.05)between C3,C4 and HDL-C. In healthy group,complement C3 negatively correlated with HDL-C,the difference was statistically significant(P<0.05).[Conclusions]In patients with coronary heart disease, the level of C3 and C4 increased,while the level of HDL-C decreased ,and inflammation may affect the relevance judgments between complement and HDL-C.