Objective To investigate the effect of dyslipidemia on retinol binding protein4(RBP4) and their correlation in type 2 diabetes(T2DM ) patients .Methods A total of 55 patients with T2DM were selected into T2DM group ,51 healthy persons were selected into control group .Serum total cholesterol(CHOL) ,high density lipoprotein cholesterol(HDL-C) ,low density lipoprotein cholesterol(LDL-C) ,triglyceride(TG) ,apolipoprotein A1(ApoA1) ,apolipoprotein B(ApoB) ,glucose(Glu) and RBP4 were detected by automatic biochemical analyzer ,and compared between the two groups ,the relationship between TG and RBP4 was analyzed .Re-sults The levels of serum RBP4 in patients with T2DM was significantly higher than that of control group .Positive correlation was observed between RBP4 and TG in patients with T2DM (r=0 .333 ,P=0 .013) ,however ,no correlation was found in patients in control group(r=0 .065 ,P=0 .614) .Serum RBP4 in patients with TG>1 .70 mmol/L were significant higher than those patients with TG≤1 .70 mmol/L (P=0 .011) .Conclusion High level of serum RBP4 was observed in patients with T2DM ,and positive correlated with TG ,which means level of RBP4 might be affected by TG .

Objective .To evaluate the role of cystatin c in the risk judgement of coronary artery disease. Methods 77 patients with coronary artery disease were involved in the study.They were divided into two groups:Group 1 were patients with angiographically diagnosed coronary artery disease ( CAD) with their first acute coronary syndrome ( n=55);Group 2 were patients who had clinically diagnosed coronary disease but were negative on angiog-raphy ( n=22) .Serum Cys C levels in the two groups and the correlation to CAD were analyzed.Results Serum Cys C levels in patients of Group 1 were higher than that of Group 2 [(1.23 ±1.07)mg/L vs.(0.71 ±0.32)mg/L,P=0.002],but no significant difference was found between the two groups in crea levels [(123.76 ±80.44)μmmol/L vs.(117.63 ±65.43)μmmol/L,P=0.230].A ROC analysis revealed that there was a significantly higher risk of ACS above a cystatin c level of 0.875mg/mL (sensitivity:84.3%,specificity:38.8%).A similar analysis carried out for eGFR did not find any significant cut-off points above which risk of ACS was higher.Conclusion The data showed that a high level of cystatin c level is a risk factor for ACS.This could play an important role in the early diag-nosis and prevention of adverse cardiovascular events.

Humans ; Liver Cirrhosis ; diagnosis ; pathology ; therapy

Animal models are of great value in the study of allergic bronchial asthma.There are a varie-ty of methods to build asthma models,and model evaluation lacks standardized criteria.By retrieval analyzing the recent articles about asthma animal experiments at home and abroad,we conduct a comprehensive assessment on the experimental animal selection,model preparation,especially establishment and evaluation of ovalbumin-in-duced models,to help on the application and optimization about asthma models.

Objective To investigate the genotoxicity of heavy rare-earth holmium to bone-marrow cells of mice.Methods Kunming mice were randomly divided into six groups and were given holmium-trioxide-HCl by gavage at several doses of 0,10,20,40,80 and 160 mg/kg bw respectively one time a day for two days;other Kunming mice were randomly divided into 3 groups and holmium nitrate was given i.p.at doses of 10,40 and 80 mg/kg bw respectively one time a day for two days.24 hours after the last treatment,the bone-marrow cells of thighbone were collected for micronuclei analysis and single cell gel electrophoresis (SCGE).Results Frequencies of micronuclei were increased by the 2 kinds of holmium solutions at the dosage from 10 to 80 mg/kg bw with a dose-effect relationship,but at the dose of 160 mg/kg bw,it was lower than the negative control.In SCGE,the average tail length increased significantly (P

Methamphetamine (MA) is a highly addictive psychostimulant that has effects on central nervous system.Chronic MA useis linked to serious neurotoxic effects and ultimately cognitive impairments, thus understanding the effect of chronic MA use on cognitive function can improve the treatment of MA abusers and reduce MA relapse. In the present study, we summarized the main results of animal experiments, neuropsychological studies and brain imaging studies on MA abuse and cognitive impairment, which may provide the basis for future research and clinical treatment.

Objective To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.\ Methods\ Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunitⅠ(mtCOⅠ) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.\ Results \ The mtCOⅠgene sequences of Lanping sample were identical to that of T\^saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCOⅠgene sequences, while the sample from Congjiang had the same mtCOⅠgene sequences as T\^saginata . The homology between these two groups of gene sequences was 97\^44%, while the homology of amino acid sequences reached to 99\^16%. The constructed phylogenetic tree revealed that the relationship between T\^saginata asiatica and T\^saginata is closer, both are distant relative to T\^solium and other species of cestodes.\ Conclusion \ The Taenia prevalent in Lanping, Dali and Duyun is identified as T\^saginata asiatica , while that isolated in Congjiang is the typical T\^saginata.

The biological principle of genetic code and genetic computation in natural selection is discussed and a new frame of genetic computation is proposed.

Objective To analyze the factors affecting the development of genome technology-based genetic testing services. Methods Purposive sampling was used to select experts in the field of genome or genetic testing. Following interview with 11 experts,data was obtained from 25 individuals and analyzed by using quantitative SWOT analysis, including the concept of SWOT method, Analytic Hierarchy Process,Internal Factor Evaluation ( IFE ) Matrix, and External Factor Evaluation ( EFE ) Matrix. Consistent ratio (CR) was chosen to test the logic. Results Of the 15 critical affecting factors, the weighting coefficient of deficiency of laws and market regulations, state supporting policies, and lack of intellectual property protection was 0. 099,0. 091 and 0. 087, respectively. The development of genetic testing services was found in the third quadrant of SWOT analysis. Conclusion These findings suggest that defensive strategy should be taken to improve genetic testing services and to reduce the risk of genetic testing.

Lipocalin-2 is an adipocytokine secreted mainly by adipose tissue. Increasing lines of evidence suggest that lipocalin-2 is an inflammatory factor associated with insulin resistence, obesity and its complications. The precise mechanism of the development of obesity-related disorders induced by lipocalin-2 is not very clear, however, it may be a useful target in drug therapy for obesity-related metabolic and cardiovascular diseases.