Objective:To realize the different disaster recovery of infectious disease net reporting system of Chinese Center for Disease Control and Prevention (China CDC) in the same city.Methods:We adopted B/S/DBMS three-tier architecture to deploy the infectious disease net reporting system in the disaster recovery center, realized data level disaster recovery through the remote replication technology, realized application level disaster recovery through technology of the data asynchronous replicating and transmitted disaster recovery data of the infectious disease net reporting system through asynchronous mode. Results: According to the existing resources, we established different disaster recovery center of the same city, realized the disaster recovery data backup of important information system in the same city, completed the security of software and hardware in information security level protection, and passed the level evaluation of Ministry of Public Security.Conclusion: The establishment of disaster recovery center in the off-sete of same city can make sure the security of the system and data, and ensure the continuity of work efficiency.

Objective To observe the clinical efficacy of acupoint application with Xiong Bi Tie in treating unstable angina pectoris. Method Sixty eligible subjects with unstable angina pectoris were randomized into a control group (treated with basic Western medication) and a treatment group (intervened by the basic Western medication plus acupoint application with Xiong Bi Tie), 30 cases in each group. The symptoms of unstable angina pectoris, syndromes and signs of traditional Chinese medicine (TCM), and cardiogram were recorded, and the clinical efficacies were also observed. Result In comparing the improvement of angina pectoris, the treatment group was better than the control group, with a statistical significance (P<0.05). From the perspective of TCM syndromes and signs, the efficacy of the treatment group was significantly better than that of the control group (P<0.05). Regarding the cardiogram, the total effective rate was 76.7% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05). Conclusion Acupoint application with Xiong Bi Tie can significantly improve the angina symptoms and cardiogram result of unstable angina pectoris (blood stagnation caused by qi deficiency syndrome), and it’s safe and easy-to-operate.

Objective To explore the role of HPV16 and p53 protein in the pathogenesis of tumors of nasal cavity and sinus of Chinese people. Methods The expression of HPV16 and p53 in 58 tumor samples of nasal cavity and sinus was detected by immunohistochemistry. Results There was no p53 expression in inverted pipillomas(IP). HPV16 infection rate in IP was 62.5%. The positive rate of p53 protein in the malignant tumors and IP with atypical hyperplasia was 56.0% and 44.4%, respectively, and HPV16 infection rate in the both tissues was 24.0% and 66.7%, respectively. The positive rate of p53 and HPV16 simultaneous expression in IP with atypical hyperplasia were 33.3%, which was higher than that in the malignant tumors and IP without atypical hyperplasia. Conclusion The high-risk HPV16 infection may be primary cause of IP. p53 gene mutation may be close relation with the pathogenesis of nasal cavity and sinus malignant tumors. HPV16 and p53 may have synergic effect in the malignant transformation of nasal cavity and sinus benign tumors.

0.05). Conclusion The p53 gene mutation has close relation with nasal malignant tumor , and may has no related to the pathogenesis of IP possible.

There is a high prevalence of gastric cancer in China,but most of the patients are diagnosed at advanced stages,when chemotherapy is regarded as an important component of the multimodal treatment.Chemotherapy has demonstrated advantages over best suppor-tive care in prolonging survival and improving quality of life.Over the past 40 years,the clinical responses of two or three-drug combination chemotherapy regimens have been accepted,and 5-fluorouracil,cisplatin and epirubicin have been most frequently used in gastric cancer.Recently,the therapeutic effects of advanced gastric cancer treated by new regimen consisted of docetaxel,irinotecan,oxaliplatin and capecitabine have been further enhanced.The molecular targeted agent,cetuximab and bevacizumab,combined with chemotherapy,would be more promising therapeutic regimens in the treatment of advanced gastric caner.

Objective To study the changes of gene and protein expressions of key components of renin-angiotensin system (RAS) in myocardium of simulated weightless rats. Method Tail-suspended rats were adopted as animal model of simulated weightlessness. RT-PCR and Western blot analysis were performed to examine mRNA and protein expressions in rat myocardium, respectively. Result After 1 week tail-suspension,no obvious changes in angiotensinogen (AGT), angiotensin converting enzyme (ACE), 1a and 1b subtype of type 1 angiotensin Ⅱ receptor (AT 1aand AT 1b), key components of RAS in myocardium were observed. But after 4 weeks tail-suspension, the expressions of AGT mRNA and higher molecular weight band of AGT protein increased significantly (P

Objective To investigate the expression of hypoxia inducible factor-1?and VEGF in lung of mice during hypoxia and its relationship with angiogenesis.Methods The mice were divided into two group(hypoxia group and control group).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5%O2 respectively for 3,6,9 days.Immuno- histochemical staining technique was used to examine HIF-1?,VEGF and MVD.The p rotein expression of HIF-1? and VEGF were observed by Western blot.Results HIF-1?proteins didn't express and VEGF proteins expressed weakly in control group mice;Compared with the controls,the expression of HIF-1?and VEGF increased dramadically.There were significant differences between the hypoxia groups and the control group;Positive relationships were found between the expression of HIF-1?,VEGF and MVD.Conclusion HIF-1?/VEGF pathway may play an important role in angiogenesis of hypoxia in the lung of mice.

ObjectiveTo evaluate the therapeutic effect of encircling constriction of superficial femoral vein in the treatment of primary deep venous insuficiency(PDVI).MethodsFrom Jan.2004 to Jun.2011,187 patients( 196 lower extremities)with primary deep venous insufficiency were treated with encircling constriction of venous wall at the first pair of superficial femoral venous valve pulse superficial varicose stripping.Results The 183 limbs in 176 patients were followed-up from 7 months to 8 years,with a mean period for 63.5 months.Ninety-four point one percent of the patients were followed up (176/187).Clinical symptomatic improvement achieved in 87.4％ ( 160/183 ) of the lower extremities.There was 2.2％ ( 4/183 ) of recurrence of the varicose vein,9.3％ ( 17/183 ) of light edema,8％ ( 2/25 ) of recurrence of ulcer.ConclusionEncircling constriction of superficial femoral vein is an effective method in the treatment of primary deep venous valve insufficiency.

BACKGROUND:Potential mechanisms of scar formation and scar prevention strategies have become more important in current medical research. Scar assessment scales have the potential to contribute to better evaluation of scar properties in both research and clinical diagnosis. OBJECTIVE: To summarize the literatures addressing scar prevention and clinical assessment. METHODS: A computer search of CNKI database from 2000 to 2014, PubMed database from 1975 to 2014 and Foreign Medical Journal Ful-Text Service Database from 2000 to 2014 was performed using the keywords of scar, prevention, treat, cicatrix, assessment, measure in Chinese and English to retrieve articles related to scar prevention strategies and scar assessment scales. Totaly 39 articles meeting the inclusive criteria were included in result analysis. RESULTS AND CONCLUSION: During wound healing, the unbalance between colagen anabolism and katabolism can lead to the formation of pathological scar. Prevention and treatment of pathological scars should be combined, and early intervention was used in wound healing. General scar assessment scales include Vancouver Scar Scale, Visual Analog Scale, Patient and Observer Scar Assessment Scale, Manchester Scar Assessment Scale, Stony Brook Scar Assessment Scale, al of which have the potential to contribute to better evaluation of scar properties in both research and clinical diagnosis.

Aim To quantitatively analyze the lymphocyte proliferation using the time-series data of carboxyfluorescein diacetate succinimidyl ester(CFSE).Methods The model of immune suppression in mice was created by cyclophosphamide and CFSE dye was used for staining the lymphocyte of both control group and treatment group animals. The data were analyzed through mathematical model-fitting.Results The first generation of cell proliferation of the control group and treatment group were 27.17 h and 22.88 h; cell death rates in each division were respectively 20% and 40%; the half-life of cells before the proliferation was respectively 31.53 h and 43.32 h.Conclusions The mathematical fitting of CFSE data can quantitatively analyze the mechanism of the drugs attecting the proliferation of lymphocyte.In this experiment the mechanism of cyclophosphamide inhibiting lymphocyte proliferation may be due to the increase of cell death rate of each division caused by cyclophosphamide.