Objective To study the effect of TanshinoneⅡA(TSN) on vascular smooth muscle cells(VSMCs) induced by high glucose and its relationship with MAPK signal pathway.Methods After the establishment of the model of diabetic rats,the aortic of rats was isolated and cultured.DNA synthesis was analyzed by ELISA of BrdU.The activity of MAPK was measured by liquid scintillation counting.Results TSN inhibited the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats in dose-dependent manner.TSN at the dosage of 0.5 mg/L significantly decreased the activity of MAPK as well as of U0126.Conclusion The effect of TSN on the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats is potentially mediated by inhibition of MAPK.

[Objective] Summing up the specific application of the three stages therapy of Professor XU Li in the treatment of cancer.[Methods]By copying the prescription and consulting the medical books of Professor XU, learning his clinical experience in treatment of cancer,summing up the core thinking of the three stages therapy and the experience of Chinese medicinal plant. [Result]By summing up the three stages therapy(assisting ,maintaining and palliative treatment),the special herb and attaching importance to develop the XIAN TIAN and HOU TIAN in the treatment of cancer ,it can improve radiation and chemotherapy therapeutic efficacy and resistance to transfer the maintenance treatment of recurrence ,improve the quality of life and prolong survival time of palliative care.By the guidance of the three stages therapy,smoothly finish the postoperative radiotherapy of nasopharyngeal carcinoma,successfully reverse the precancerous lesions of chronic atrophic gastritis with intestinal metaplasia,the two typical medical cases have significant curative effect. [Conclusion] Taking the three stages therapy in the process of the treatment of the tumor and precancerous lesions ,it can get good clinical effect,it is worth spreading in clinic.

Objective: Digital images processing is widely applied in the fields of biology and medicine, and some achievements have been gained by this technique. But there is few reports on measure the parameters of morphology of pathology from the patients which were threated by ovulation induction, by computer processing techniques. The work shows it has significant value in clinical diagnosis and reproductive medicine at present and in the future. Methods: Write the programmeces by Image Processing Toolbox in MATLAB. The original pathology images are preprocessed by 4 steps: step 1: read imagejstep 2: imopen the curve background brightness; step 3: adjust the background; step 4: adjust the contrast. Then segmentation image by 4 steps; step 1: read image; step 2: estimation the image; step 3: remove the noise; step 4: find the boundaries. The end, measure the parameters of morphology. Results: The pathology was studied percase for their area, perimeter, maximum diameter, equivalent diameter and metric. Conclusions: Image Processing Toolbox is useful to measure the parameters of morphology of pathology.

Objective:To compare the content differences of 18 amino acids in plancenta histolysate determined by pre-column de-rivatization HPLC and post-column derivatization cation-exchange chromatography ( AARO) . Methods: The HPLC method was per-formed on a C18 column and 2, 4-dinitro chlorobenzene ( DNCB) was used for pre-column derivatization, and then the determination was carried out after adding 0. 1 mol·L-1 borax buffer (pH=9. 1), and the AARO method was used for the direct determination with a strong acid cation-exchange chromatographic column and post-column derivatization. Results: The RSD for reproducibility of the AARO method was 1. 84%-0. 91%, while that of the HPLC method was 1. 87%-1. 04%. Conclusion:Both AARO method and HPLC method can be used for the quantitative determination of 18 amino acids in plancenta histolysate with the similar results. However, pre-column derivatization HPLC method may cause incomplete derivatization and instable derivatives.

Objective To explore the application value of the diffusion-weighted imaging (DWI)and dynamic contrast-enhanced MRI in detection of peripheral zone of hepatic alveolar echinococcosis (HAE).Methods Twenty patients with HAE underwent abdominal plain scan,DWI and dynamic enhanced MRI.The ADC values and enhanced ratio of the central necrosis,solid component,peripheral zone and normal liver parenchyma were measured at a workstation,and further were analyzed and compared.Results 32 lesions were detected in 20 patients with HAE.Two groups were divided according to the lesion with central necrosis (group A)or not (group B).In group A, the ADC values of the solid part and normal liver parenchyma were (1.52±0.30)×10 -3 mm2/s and (1.22±0.30)×10 -3 mm2/s. Meanwhile,in group B those were (1.50±0.25)×10-3 mm2/s and (1.03±0.28)×10-3 mm2/s,exhibiting no statistical differences between the two groups.In group A and B,the ADC values of the peripheral zone of lesion were 1.34(1.10,1.61)×10 -3 mm2/s and 0.96 (0.86,1.22)×10 -3 mm2/s,exhibiting statistical differences (Z =2.867,P =0.004);meanwhile,the enhanced ratio were 1.70± 0.36 and 1.58±0.30,exhibiting no statistical difference.No correlation between ADC values and enhanced ratio was found in the peripheral zone.Conclusion DWI at 3.0T can display visually the HAE,and the ADC value may reflect the DWI features of periph-eral zone.The ADC values in combination with enhanced ratio may help for the diagnosis.

Using immunohistochemical method,the antigens were shown to be mainly located in the cecum,tegument,esophagus,pharynx,excretory bladder,uterus and oral sucker of Pagumogonimus skrjabini.Histochemical analyses showed that antigens contained enzymes such as acid phosphatase (ACP),alkaline phosphatase (AKP),and acetyleholinesterase (AChE).Other chemical substances,for example,alkaline protein,basic protein,glycogen and acid mucopolysaccharides were also detected in the antigens of P.skrjabini.

By literature research and questionnaire, the paper analyzed development mode and status of scientific researching institutions of Traditional Chinese Medicine in other countries, discussed necessity and realistic meaning of establishment of the standards, and advocated the standards must be followed the rule of standing out characteristic of traditional Chinese medicine, making use of modern scientific technology and instrument, emphasizing commonness, classifying instruction and getting hold of major point.

The utility of anticoagulants is a significant content of total quality management(TQA)of clinical laboratory.The accurate results of laboratory investigations are closely correlated witll appropriate application of blood anticoagulants.The paper reviews the utility field and effects on investigation result of different anticoagulants,and it also raises the current problems of using anticoagulants and Dut forward corresponding solutions.

Objective To detect serum concentrations of Dickkopf-1(DKK-1) and soluble receptor activator of nuclear factor-κB ligand (sRANKL) in patients with multiple myeloma (MM) and to investigate its clinical significance. Methods Serum DKK-1, sRANKL, osteoporotegerin(OPG) and tartrate-resistant acid phosphatase 5b (TRACP-5b) levels were quantified in 30 newly diagnosed MM patients and 20 healthy control subjects by using sandwich ELISA. Results The serum DKK-1, sRANKL,OPG and TRACP-5b levels were significantly higher than those in the healthy controls (42.96 μg/L vs 5.33 μg/L, 1.83 pmol/Lvs 0. 79 pmol/L, 1799. 30 pmol/L vs 822.40 pmol/L, 5.81 U/L vs 0. 28 U/L, respectively; all P＜0. 05). Serum levels of DKK-1 were positively correlated with sRANKL and TRACP-5b, respectively.Serum concentrations of DKK-1 and sRANKL were significantly elevated in stage Ⅲvs stages Ⅰ and Ⅱaccording to International Staging System (ISS) (46. 33 μg/L vs 37.91 μg/L, 2.26 pmol/L vs 1.19pmol/L, respectively, all P ＜0.05). Serum concentrations of DKK-1 , sRANKL and TRACP-5b were significantly higher in patients with more than 3 lytic bone lesions than those with only 1-3 lytic bone lesions (46. 30 μg/L vs 31.98 μg/L, 2. 18 pmol/L vs 0. 69 pmol/L, 6.02 U/L vs 5. 13 U/L, all P ＜ 0.05).Conclusions MM patients have increased serum DKK-1, sRANKL, OPG and TRACP-5b levels as compared with the healthy controls. Serum concentrations of DKK-1 and sRANKL have close relationship with MM stage and lytic bone disease.

Chronic cough is one of the common symptoms of pediatric respiratory. The diagnosis of chronic cough is complicated, and the curative effect is not clear. At present, studies on chronic cough in children are still in the preliminary stage. This article summarized the etiology, pathogenesis and clinical treatment of chronic cough.