Objective To investigate the effect of autologous bone marrow mononuclear cells (BM-MNCs) implantation on neovascularization in rat model of hind limb ischemia, and explore its mechanism. Methods Rat BM-MNCs were isolated from tibia and femur using density gradient centrifugation. A rat model of ischemic hind limb was made by the ligation of the right femoral artery and its branches in imbred Wistar rats. BM-MNCs were injected into 7 points of the ischemic muscles (20?l/point). The levels of VEGF, bFGF and IL-1? in the ischemic musles at the third, 7th and 14th days after ligation were measured by ELISA. Micro-vessel density (MVD) in the ischemic mulsles was evaluated at the second and 4th weeks after ligation. Results The levels of bFGF and IL-1? in the implantation group were significantly higher than those in the control group. The MVD of ischemic mulsles in the implantation group was also significantly higher than that in the control group at the second and 4th weeks after treatment. Conclusion The implantation of autologous BM-MNCs could induce neovascularization in a rat ischemic hind limb, and bFGF and IL-1? secreted by BM-MNCs might play a role in the neovascularization.

Objective To study the effect of pulmonary artery perfusion with the modified low-potassium dextran(LPD) solution on cardiopulmonary bypass(CPB)-induced systemic inflammatory response. Methods 30 patients undergoing mitral valve replacement were randomly divided into control group (n=15) and lung perfusion group (n=15). The patients in the lung perfusion group were perfused with the modified LPD solution by pulmonary artery during CPB. The patients in the control group underwent the routine procedure of mitral valve replacement. The plasma concentrations of TNF-? and IL-8 were measured before and 0 h, 6 h after surgery by ABC-ELISA. Results The plasma levels of TNF-? and IL-8 significantly increased after surgery in both groups. The plasma levels of TNF-? and IL-8 were significantly lower(P

Objective To explore the effects of mirror therapy combined with Tongdu Xingshen acupuncture on the upper extremity function of stroke survivors.Methods Sixty stroke survivors were randomly divided into a mirror group (n =20),an acupuncture group (n =20) and a combined group (n =20).In addition to routine rehabilitation treatment,those in each group received mirror therapy,acupuncture or both for 4 weeks.Before the treatment and after 2 and 4 weeks of treatment,all of the patients were assessed using the Fugl-Meyer motor function assessment (FMA),Brunnstrom upper limb and hand staging (BSULH),the Barthel Index (BI) and in terms of the active range of motion in the wrist contralateral to their hemiplegia (AROM).The combined group was also evaluated using functional magnetic resonance imaging (fMRI) before treatment and after the 4 weeks.Results After 2 and 4 weeks of the treatment,the average BI,AROM,FMA and BSULH results had improved significantly compared with before the treatment.The improvements in the combined group were significantly greater than in the other groups after 2 and 4 weeks.The fMRI demonstrated that there were high-intensity signals in the primary motor area,the premotor area and the supplementary motor area on the affected side after 4 weeks of treatment.Conclusions Mirror therapy combined with Tungdu Xingshen acupuncture can improve the functional performance of the upper extremities of stroke survivors.

OBJECTIVE: To evaluate the efficiency of video-assisted thoracoscopic surgery for primary palm hyperhidrosis by T4 sympathicotomy plus bypass fiber resection, and to describe our experience regarding the prevention of related postoperative complications. METHODS: Clinical data for 32 cases of primary palmar hyperhidrosis in patients who underwent bilateral video-assisted thoracoscopic sympathictomy plus bypass fiber resection from October 2008 to June 2011 were analyzed retrospectively. RESULTS: The operation was performed successfully on all patients, and their palmar hyperhidrosis was completely alleviated after operation. No severe, surgery-related, postoperative complications occurred. CONCLUSION Video-assisted thoracoscopic sympathictomy is an effective, safe and minimally invasive procedure for primary palmar hyperhidrosis.
Adolescent ; Adult ; Female ; Hand ; Humans ; Hyperhidrosis ; etiology ; surgery ; Nerve Fibers ; Postoperative Complications ; prevention & control ; Sympathectomy ; methods ; Thoracic Surgery, Video-Assisted ; methods ; Young Adult

OBJECTIVE: To summarize the characteristics of reoperative valve surgery after previous open-heart valve surgery. METHODS: From 1996 to 2010, 155 patients who underwent reoperative valve surgery, either valve replacement or tricuspid annuloplasty or the repair of perivalvular leakage were included in the study. The reoperative interval was 1-266 (94.82 ± 85.37) months. All surgeries were carried out with extracorporeal circulation under moderated hypothermia. The cardioplegic solution in cold crystal or blood was used if heart beating was stopped during the surgery. RESULTS: The total in-hospital mortality was 5.81%, while it was 2.75% from 2005 to 2010. The end-diastolic dimension, size of atrium and ventricles were reduced after the reoperation. Ventricular arrhythmia and low cardiac output were the most frequent complications. CONCLUSION The success rate of reoperative valve surgery can be improved by the distinctive therapeutic strategies based on the clinical characteristics and therapy principles obtained from practice experiences.
Adolescent ; Adult ; Aged ; Cardiac Output, Low ; etiology ; Cardiopulmonary Bypass ; Child ; Female ; Heart Valve Diseases ; mortality ; surgery ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; adverse effects ; methods ; mortality ; Humans ; Male ; Middle Aged ; Reoperation ; Tachycardia, Ventricular ; etiology ; Young Adult

OBJECTIVE: To evaluate the safety and efficacy of modified technique for removing Nuss bar after Nuss procedure. METHODS: We reviewed 186 patients undergoing bar removal after repair of pectus excavatum with Nuss procedure at our institution from December 2008 to February 2012. All patients had unilateral incision (metallic stabilizers have been used on one side in all patients). Under general anesthesia with single lumen tracheal tube or laryngeal mask, with the patient lying down in supine position, the bar was pulled out along the thoracic wall without overturning or straightening. RESULTS: Totally 132 patients (71.0%) had the bar removed 2 years after the Nuss procedure, 1 (0.5%) removed within 1 year and 53 (28.5%) removed over 2 and half years. The operation time for bar removal was 9-20 (13.1 ± 3.4) min, and the operative blood loss was 3-20 (5.2 ± 2.7) mL. There was no hemorrhage. Three patients (1.6%) developed mild pneumothorax and none showed infection of incision after the operation. All patients were discharged 1 day after the surgery and followed up for 4-48 (21.4 ± 6.8) months. Recurrence was found in the one who which had the bar removed within 1 year (0.5%). CONCLUSION With modified procedures, Nuss bar can be easily and safely removed 2 years or longer after the Nuss operation. After removing the metallic stabilizer, the bar should be turned and then pulled out along the original surgical incision without bending or turning.
Adolescent ; Blood Loss, Surgical ; Child ; Child, Preschool ; China ; epidemiology ; Device Removal ; methods ; Female ; Funnel Chest ; surgery ; Humans ; Male ; Orthopedic Fixation Devices ; Pneumothorax ; epidemiology ; etiology ; Postoperative Complications ; epidemiology ; prevention & control ; Prostheses and Implants ; Sternum ; surgery ; Young Adult