Objective To optimize conditions for extraction of alkaloids from Evodia rutaecarpa by means of 732 cation exchange resin and performe its anti-breast cancer bioactivity.Methods The optimum processing route on extraction of alkaloids from Evodia rutaecarpa was investigated by means of 732 cation exchange resin.The performance of 732 cation exchange resin was compared with tranditonal process ( aqueous extraction-ethanol precipitation in extraction-purification process).The alkaloid reagent-potassium mercuric iodide test and MTT test were performed on the crudes.Results Compared with traditional purification process, it was much better to use 732 cation exchange resin approach for extraction of alkaloids from Evodia rutaecarp with high yield(16.81%) The best eluent should be saturated brine with excellent purification.No obvious correlations were found between the toxin of breast cancer cell and concentration of total alkaloids.When the concentration of total alkaloids was 50μmol/mL, cellular survival rate was 68%afterward 24 h.When the concentration of total alkaloids comes to 100 μmol/mL and 150 μmol/mL, cellular survival rates were slightly decreased by 66% and 60%.Conclusion 732 cation exchange resin performes much higher than traditional process in purification of total alkaloids extracted from Evodia rutaecarpa.Simultaneously, the extracted total alkaloids showe remarkable inhibition in breast cancer cell.

Bone formation on hydroxyapatite (HA) coating in the presence of gaps is important for clinical application. Pure Ti and hydroxyapatite coated by plasma sprayed samples were implanted in dog respectively. The implants were surrounded by gaps of 2 mm, and the follow-up period was 12 weeks. Histological examination and histomorphometry revealed that gaps could be bridged by bone provided the hydroxyapatite coating was applied, and that pure Ti implants were surrounded by fibrous tissue with no bone contact at all.
Animals ; Bone Substitutes ; Coated Materials, Biocompatible ; pharmacology ; Dogs ; Femur ; injuries ; surgery ; Hydroxyapatites ; pharmacology ; Implants, Experimental ; Male ; Osseointegration ; Osteogenesis ; drug effects ; Titanium

In order to solve the problesm in biological sealing of load-bearing percutaneous implants for a fairly long time, we investigated titanium with bioactivated anodic oxidized surface(group A) through the animal tests in vivo and the epithelium cell culture in vitro. Smooth Ti (group B) was used as control. The animal tests results showed that there was no evident difference in the inflammory reaction between the group A implant tissues and the group B implant/tissues. The bioactivated Ti surface could keep the implant not only bonding with the bone firmly but also adhering to the soft tissue closely, thus contributing to the formation of calcium phosphate layer and its micropores. The cell culture results also demonstrated that the microporous surface of group A could clasp and fix the skin. So, it can be concluded that the surface modified method of anode oxidization may be one of the most effective methods to resolve the problem of durable biological sealing.
Animals ; Biocompatible Materials ; chemistry ; Cells, Cultured ; Coated Materials, Biocompatible ; Epithelial Cells ; drug effects ; Materials Testing ; Osseointegration ; Prostheses and Implants ; Tibia ; surgery ; Titanium ; chemistry