Objective　According to Kaschin-Beck Disea se monitory standardization that had been adjusted by our country,we monitored the state of Kaschin-Beck Disease in Gansu province.Methods　So as to understand change of illness,we took methods of epidemiological investigation,clinical examination and X-ray diagnosis.Results　It is not detected in the clinical that patient suffered from more than I of KBD among 7～12 years old in Qingyang monitory netw ork.X-ray detectable rate is 3%,but 12 cases patients were showed in Zhangjiach uan.X-ray detectable rate is 22.22%.Conclusions　Illness was showed steady state and was con trolled in Qingyang region,but illness recurred clearly in Zhangjiachuan region.

Objective To observe the effect of microprismatic needle scalpel(MPNS) on headache induced by compression of greater occipital nerve or lesser occipital nerve.Methods 95 patients with headache induced by compression of occipital nerve were divided into the treatment group (50 cases, treated with MPNS) and control group (45 cases, treated with carbamazepine).Results In the treatment group, 42 cases were cured, 6 cases were effective, 2 cases were ineffective; while, those in the control group were 16 cases, 21 cases and 8 cases respectively. There was a significant difference between two groups ( P<0.01).Conclusion MPNS has an obvious effect on headache induced by compression of occipital nerves.

OBJECTIVETo estimate the number of death from malignant tumor and to assess the completeness of cancer registry data using the capture-recapture method.

METHODSData about death form malignant tumor were collected during the period of 2004 and 2005 from cancer registry, civil administration and police registries in Linzhou. Cases in every source were matched on the address, name, sex, ID card, date of death, cause of death. A 3-source log-liner poisson model was used to estimate the real number of death from malignant tumor during the period of 2004 and 2005 in Linzhou.

RESULTSThe real number of death from malignant tumor that estimated by capture-recapture method was 3140 (95% CI: 3124 - 3161) during 2004-2005 year in Linzhou. Underreported number by source linkage was 71. Log-liner model demonstrated positive dependence between the 3 sources, implying that 2-source model would underestimate missing cases. Using the mortality number estimated by the capture-recapture as the denominator, under-reporting rate of cancer registry was 6.6%.

CONCLUSIONIt is feasible to estimate the real number of death from malignant tumor and to correct data bias by applying the capture-recapture method.

Objective To discuss the effect of the family-nurse health education on the management of patients with pre-hospital pressure sores.Methods A total of 110 pre-hospital pressure sores patients were randomly divided into control group (n =55) and treatment group (n =55).Families of the treatment group participated directly in the nursing processes and the follow-up health education on the basis of regular education,while patients of the control group received regular education and their families received admission education and discharge instructions etc.The intervention lasted for three months,and the changes of both the patients and their families' mastery of rudiments of pressure sore,their nursing skills and the re-occurrence of pressure sore were observed.Results No significant difference was found in the cases of different level of pressure sore before the intervention between treatment group and control group(Ⅰ:4 vs 6,Ⅱ:22 vs 24,Ⅲ:19vs 16,Ⅳ:10 vs 9;u =0.796 7,P ＞ 0.05).After the intervention,patients and their families' awareness rate of rudiments of pressure sore and of relevant nursing skills etc.in treatment group was better than that in control group,and the difference was statistically significant (u =5.612 5,P ＜ 0.05).The reoccurrence rate of pressure sore of the treatment group was significantly lower than that of the control group (no reoccurred:45 vs 12,Ⅰ:4vs12,Ⅱ:5vs19,Ⅲ:1vs10,Ⅳ:0vs2;u=1.8526,P＜0.05).Conclusions The family-nurse health education for the patients with pre-hospital pressure sore during the nursing process and follow-up education is more helpful for the families to grasp the rudiments of pressure sore and the nursing sills that can prevent the occurrence of pressure sore,thus lowering the reoccurrence rate of pressure sore.

OBJECTIVETo analyze the distribution of single nucleotide polymorphisms (SNP) of growth hormone receptor (GHR) gene in Chinese Han ethnic population.

METHODSThe sample of 106 unrelated healthy Chinese Hans was studied by sequencing exons of the GHR gene to detect SNP.

RESULTSThere were 6 SNP spots identified in exon 6 and exon 10. Five of them were found in exon 10, and one in exon 6. There were differences between the allele frequencies of the SNP we found and those in the NCBI database. The highest heterozygosity of the SNP was found at 1630 A > C (I526L), which was 47.6%. The SNP 1483 A > C (P477T), 1735 A > C (P561T) and 1319 G > T (C422F) had polarity change. The SNP 536 A > G in exon 6 from the NCBI database was not detected in this population. The allele distribution of SNP was in good unity with the Hardey-Weinberg equilibrium.

CONCLUSIONIt is suggested that the SNP of GHR are unevenly distributed and different in different ethnic populations.

The purpose of this study was to investigate the efficacy of treatment with haploidentical donor's lymphocyte infusion(hiDLI) combined with interleukin-2 (IL-2) after transplantation of autologous peripheral blood stem cells mixed with haploidentical allogeneic bone marrow (mix-HSCT) for acute myeloid leukemia (AML). 49 patients diagnosed as AML were enrolled in this study. After preconditioning with TBI plus VEMAC regimen, all patients received mix-HSCT. Autologous peripheral blood hematopoietic stem cells were mobilized with chemotherapy-combined G-CSF, and haploidentical allogeneic bone marrow cells were not mobilized with G-CSF. 33 patients in test group were treated with hiDLI plus IL-2 for 1-8 times after hematopoietic reconstruction, 16 patients in control group received mix-HSCT only. All the patients were followed-up for more than 3 years. The results showed that all the patients obtained hematopoietic reconstruction, and no graft-versus-host disease (GVHD) was found. In two groups, the median time of absolute neutrophil count (ANC) ≥ 0.5×10(9)/L was 14 (12 - 18) and 14 (11 - 16) days, and WBC count ≥ 4.0×10(9)/L was 17 (16 - 22) and 18(17 - 20) days, Plt count ≥ 50×10(8)/L were 25 (24 - 29) and 25 (23 - 26) days. 9 patients in test group formed mixed chimerism (46XX/46XY) and sustained about 3 - 12 months; disease-free survival (DFS) was 63.6%, 3 patients in control group formed mixed chimerism (46XX/46XY), persistent about 3-6 months; DFS was 50.0%. It is concluded that treatment with hiDLI plus IL-2 after mix-HSCT for AML patients may increase DFS efficiently.
Adolescent ; Adult ; Female ; Hematopoietic Stem Cell Mobilization ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunotherapy, Adoptive ; Leukemia, Myeloid, Acute ; therapy ; Male ; Transplantation, Homologous ; Young Adult

Objective To find out the distribution pattern of environmental fluoride in the coal-burning endemic fluorosis areas to provide scientific evidence for establishing prevention and remedial measures in Chongqing. Methods According to historical data in Chongqing In 2008, 4 endemic villages(Lingyun and Lizi Villages of Wushan County, and Taiping and Daqing Villages of Pengshui County) and 2 non-endemic villages (ShuangLou and XianLong Villages of Yongchuan County) were investigated. Dental fluorosis of children in 8 to 12 year old and residents over 16 years of age for clinical skeletal fluorosis were examined in four endemic villages. Five households from each of 6 villages were taken, where 500 g of coal, mixed clay with coal, mixed coal of soil, coal cinder, soil were sampled; 15 people were taken in each village, each household gathering 500 g of corn, rice, potatoes, vegetables, grain and vegetable, 100 g of dried pepper and 250 ml of drinking water were sampled from 15 families of each village. Household drinking water samples were collected 1, each 250 ml. For those having tea-drinking habit, each household was collected 50 g of tea and 600 ml of drinking tea, the amount of fluoride were determined. Indoor and outdoor air was collected and measured in 5 households in each village. Results The detected rate of dental fluorosis of children in endemic areas was 74.65% (736/986). The detected rate of skeletal fluorosis of adult was 7.20%(736/986). The average fluoride content of coal, mixed clay with coal, mixed coal of soil, cinder coal, soil in the endemic villages was (310.56±209.46), (360.51±224.96), (293.62±65.15), (186.59±133.66), (497.54±294.70)mg/kg. The average fluoride content in non-endemic villages was (48.68±10.62), (275.66±62.69), (152.20±34.43), (209.14±188.66),269.98±58.21)mg/kg. The fluoride content level of endemic villages was significantly higher than that of non-endemic villages(t=7.67,31.54,5.82, 5.82, all P<0.05). The average fluoride content of drinking water, corn, pepper, flee, potato and vegetable in the endemic villages was (0.30±0.14)mg/L, (1.83±2.67), (23.50±91.80), (0.77±0.25), (0.44±0.11), (0.48±0.18)mg/kg, The average fluoride content in non-endemic village was (0.18±0.06)mg/L, (2.21±0.46), (2.82±2.51), (1.31±0.21), (0.64±0.41), (1.10±0.77)mg/kg. The fluoride content in drinking water and pepper in the endemic villages was significantly higher than that of the non-endemic villages(t=7.79, 2.33, all P<0.05). The fluoride content of rice, potato and vegetable in the non-endemic villages was significantly higher than that of the endemic villages(t=39.29,4.69,4.01, all P<0.05). There was no significant difference of fluoride content of tea and drinking tea between endemic villages[(99.41±55.83)mg/kg, (1.59±0.91)mg/L] and non-endemic villages[(79.95±43.78)mg/kg, (1.80±1.16)mg/L, t=1.01, 0.27, all P>0.05]. The amount of drinking tea in the endemic village[(1.45±0.68)L/d] was higher than that in non-endemic village[(1.00±0.47)L/d, t=4.27, P<0.05]. The average fluoride content of indoor air in the endemic village[(12.77±8.08)μg/m3] was higher than that in non-endemic village [(1.16±1.08)μg/m3, t=9.49, P<0.01]. There was no significant difference of fluoride content of outdoor air between endemic village and non-endemic village[(1.10±1.57), (0.39±0.31)μg/m3, t=2.01, P>0.05)]. Conclusions The fluoride source of coal-burning endemic fluorosis areas are coal and mixed coal of soil in Chongqing. Fluoride enters into human bodies mainly via respiratory, not from food. Although fluoride is rich in pepper, people don't eat it, so reducing the fluoride content in indoor air is the principle measure. Drinking tea may be was one factor of endemic fluorosis, which needs to be further studied.

Objective To assess the relationship between fractional esterification rate of high density lipoprotein cholesterol(FERHDL)and coronary artery disease.Methods A total of 131 hospitalized patients underwent coronary angiography due to chest pain were included in the study and Datients were divided into CAD group(n=76)and non CAD group(n=55)according to coronary allgiograrn.Clinical and laboratory data including biochemical laboratory,FERHDL and lipid subclasses were analyzed.Results The FERHDL value of CAD group was significantly higher than that of the non CAD group(21.70±8.73 vs.18.65 4±6.26,P<0.05).There was an increased trend of FERHDL with numbers of diseased coronary arteries,significant difference was evidenced between non CAD group and 3-vessel group(18.65±6.26 vs.24.00±9.22,P<0.05).FERHDL was positively correlated with TG(r=0.647,P<0.001).LDLb-C(r=0.441,P<0.001)and negatively correlated with HDL-C(r=-0.708,P<0.001)and HDL2-C(r=-0.748,P<0.001).Conclusion Our data showed that the values of FERHDL were significantly increased in CAD patients and correlated with the severity of the CAD.

Objective To analyze the relationships between MRI image changes of lateral pterygoid muscle (LPM) , disc position and condylar mobility. Methods Twenty Class Ⅱ1 patients were chosen and MRI images of LPM were taken. The relationships between MRI image changes of LPM, disc position and condylar mobility were analyzed. Results The rate of MRI image changes of LPM in Class Ⅱ1 patients was about 45%. No significant correlations were found between MRI image changes of LPM, condylar mobility and disc position. Conclusions MRI image changes of LPM in Class Ⅱ1 patients was common hut no significant correlations were found between MRI image changes of LPM, condylar mobility and disc position.

Objective To treat simple malocclusions preliminarily using Chinese-made invisible orthodontic aligners and discuss the indications, problems existed and future development. Methods Forty-one cases with different malocclusions were selected, including crowding, spaces and spaces due to periodontal problems. Invisible aligners were made and worn by patients and they were changed every 2-3 weeks. Results Acceptable treatment results were obtained in all cases, with nice alignments and good overbite and overjet. Treatment time ranged from 6-25 months. Conclusions Indications of this technique were still limited and the technique needed to be further developed in the future.