OBJECTIVE　A RP-HPLC method was establised for the determination of paeoniflorin and geniposide in Zhonggan oral liquid.METHODS　The c18 column was used.The mobile phese was consisted of CH3CN-0.1% H3PO4(15∶85).The detection wavelength was at 230 nm.RESULTS　The average recoveries were 98.78%(RSD=2.43%,n=5)for paeoniflorin and 99.60%(RSD=1.88%,n=5)for geniposide,respectively.CONCLUSION　The method was simple,rapid and accurate.

This study was aimed to observe the therapeutic effect of liver cirrhosis treated with Tibetan medicine . A total of 70 liver cirrhosis identified cases were orally administrated with Tibetan medicine for 45 days. The results showed that the cure rate, improvement rate, ineffective rate, and total effective rate were 24 . 29% ( 17/70 ) , 67 . 14% ( 47/70 ) , 8 . 57% ( 6/70 ) , and 91 . 43%. There were no significant differences on the routine tests of blood , urine , stool and renal function before and after the treatment . It was concluded that Ti-betan medicine has unique therapeutic effects in the treatment of liver cirrhosis without any obvious poisonous or side effect .

Objective To investigate the effects of Xanthoceraside on the learning and memory impairment induced in mire by innacere-broventricular injection of aggregated amyloid β peptide _(1-42)(Aβ_(1-42)). Methods Learning and memory functions in mice were examined us-ing step-through test and water maze test. Biochemical determination of acetylcholinesterase (AchE) and choline acetyl transferase (ChAT) were measured with spectrophotometric melhod. Results Administration of Xanthoceraside reduced number of error and prolonged the laten-cy in step-through test in mice impaired by Aβ_(1-42) (P < 0.05,P< 0.01,respectively). In water maze test,the swimming time decreased in mice treated with Xanthoceraside compared with the model mice impaired by Aβ_(1-42) (P< 0.05,P< 0.01,respectively). The results of bio-chemical determination showed that decrease level of AchE and ChAT in mice impaired by Aβ_(1-42) were significantly ameliorated by Xantho-ceraside administration (P < 0.05 and P < 0.01). Conclusion Xanthoceraside has the effect of improving learning and memory impairment in mice inducel by intracerebroventricular injection of Aβ_(1-42) via enhancing the cholinergic system functions.