Objective To analyze the literature distribution and research status of depression in the elderly published in Chinese Medical Association journals.Methods The literatures of elderly depression published in 85 kinds of Chinese Medical Association journals from 2003 to 2012 were collected.The publication year,distribution,content,author,institution and region of these literatures were analyzed.Results From 2003 to 2012,there were 85 articles on depression in the elderly published in 15 kinds of journals of the Chinese Medical Association.These literatures were published mostly in 2011 (18 articles,21.2％),secondly in 2010 (13 articles,15.3％) and 2012 (12 articles,14.1％),which were published mostly in Chinese Journal of Geriatrics (29,34.1％).These articles mainly included basic science,inventory survey,diagnostic methods and clinical treatment strategy,and the most published form was treatise (77 articles,90.6％).The author who published the most articles was YUAN Gui yong from Nanjing (3 articles,3.5％),the institution which published the most articles was People's Hospital of Hainan Province (4 articles,4.7％),and the provinces or cities which published the most articles were Beijing (15 articles,17.6％),Shandong (9 articles,10.6％) and Zhejiang (7 articles,8.2％) by turns.Among the 85 articles,31 articles (36.5％) were supported by foundations.The definition,epidemiology,mechanism,influence factors,clinical characteristics,diagnosis and treatment strategy of elderly depression,and especially secondary depression were well discussed in these literatures.Conclusions The literatures of elderly depression published in Chinese Medical Association journals are insufficient in quantity,and more than half of the literatures are published from 2010 to 2012.The distribution of journals is relatively concentrated,but the distribution of author,institution and region in these articles is dispersed.Secondary depression in the elderly is concerned especially.

Objective To investigate the situation and development of international literatures on geriatrics and gerontology in mainland China.Methods The literatures on geriatrics and gerontology written by mainland Chinese scholars as the first author were selected from Thomson Scientific database in 1979-2011 period.Researches on basic theory,summary reports of clinical practice and reviews were collected.The quantity of published articles,article types,international cooperation research and funding programs,hospital rankings,as well as the 2011 geriatrics professional journals,thesis topic disciplines distribution and high-performance papers were analyzed by Thomson Data Analyzer software.Results From 1979 to 2011,the quantity of literatures written by Chinese scholars as the first author (not including Hong Kong,Macao and Taiwan regions) was 1927,including 1769 research articles,86 reviews,18 editorials,54 letters.Average paper was cited 7.6 times and the average number of article citation was 30.6.International cooperation research articles occupied 379 (19.7 ％).Articles with research sponsorship were 734 (38.1 ％),in which most subject discipline was geriatric and gerontology.Conclusions The development of geriatric medicine in China is rapid.The international scientific and technological cooperation is very extensive.It is important to further improve the world impact of geriatric medicine from China.The development of geriatric medicine in a large,cooperative,comprehensive,interdisciplinary,interagency direction,and the prospective,multicenter,cooperative study need to promote.It is extremely urgent to establish the English version of Chinese journals of geriatrics with a international influence.

The distributioa of Se in Hb and the effects of Se intake and its chemical forms oil the distribution were studied. The results showed that Se in human Hb was all located in globin and divided equally in a and ? chains. However, a part of Se could fall from Hb during the process of isolation when more Se as SeMet was taken and "excess" Se appeared in Hb. It was implicated that there were at least two kinds of linkage between Se and Hb --tight and loose.Regarding the components of amino acid in ? and ? chains, we assumed that a tight linkage between Se and Hb was in the form of SeMet, which was incorporated into Hb by dietary Se during the synthesis of Hb; the loose linkage was formed by a part of "excess" Se and CyS, especially No.93 CyS in ? chain, being unstable sclcnotrisulf ide or selenopersulfide, which broke easily by reduction during isolation. They might be utilized first by human body when Se was required.

Objective To analyze the articles published in Chinese Journal of Geriatrics from 2008 to 2012 so as to understand the characteristics of published papers and research progress in geriatric medicine.Methods Based on Wanfang database,bibliometric analysis was conducted to analyze the quantity,types,authors,affiliations,fund support of all the articles published from 2008 to 2012 in Chinese Journal of Geriatrics.Results A total of 1567 papers were published in the journal from 2008 to 2012.The column with the most published papers was clinical research (1008 papers,64.3 ％),followed by experimental researches,reviews and lectures,investigations etc.The discipline with the most articles published in the journal was cardiovascular medicine,followed by surgery,neurology; respiratory etc.1356 authors from 506 affiliations published paper as the first author in the journal during this five years,with the maximal number being 7 papers and the major affiliation being affiliated hospitals of medical colleges (733 papers,49.3 ％).Authors from Beijing,Zhejiang,Jiangsu,Shanghai published more papers than those from other provinces.29.0％ (455papers) of the total papers were supported by fund,among which the majority was provincial fund,followed by national fund,municipal fund,ministry fund and military fund.Conclusions A relatively stable core author group has formed for Chinese Journal of Geriatrics,and the articles are of high quality.Chinese Journal of Geriatrics is one of the highest academic journals in geriatric medicine.

Objective To understand the research status and development tendency of domestic geriatrics.Methods To analyze all the key words in the academic articles from five representative Chinese journals of geriatrics from 2009 to 2013 through key words frequency analysis.Results From 2009 to 2013,the 55212 key words in 15314 articles were analyzed.The percentages of the articles indexing 3,4 and 5 keywords were 39.3％ (6012/15314),33.3％ (5092/15314) and 13.9％(2123/15314),respectively.There were 45 high-frequency keywords,among which 17 key words (37.8％) were in the top 30 every year.The main research object of geriatrics was Aged,the main research contents were common diseases in the elderly,including Diabetes Mellitus,Coronary Artery Disease,Alzheimer's Disease,Hypertension,Cerebral Infarction,Cognition Disorders and etc.The Risk Factors,Therapeutics and Prognosis of these common diseases were always explored.Co-words analysis showed that there were 51 pairs of key words with a frequency more than or equal to 20.Conclusions The hot-spot of geriatric research in our country is relatively concentrated.We should pay attention to body disease,and focus on the mental health problems in the elderly.

Objective To construct the KU80 inhibition cell model by RNAi in U2OS cell and to explore the relationship between the Ku80,telomeres and radiosensitivity in telomerase-negative tumor cells.Methods U2OS cells were transfected with the recombinant plasmids of pshRNA-K80 by the lipofectamine,and the stable transfected cell clones were selected by G418.After the selection,the cells were collected and analyzed by the flow cytometry.RT-PCR and Western blot were used to measure the expression of Ku80 and Real-time PCR was used to detect the length of telomeres.The radiosensitivity of U2OS was determined by clone formation array.Results The transfection efficiency of the positive cell clones detected by the flow cytometry was (83.23 ± 7.63) ％.The inhibition rate of the Ku80 gene transcription in the cell group with recombinant plasmid was(68.09 ± 1.16)％ and the inhibition rate of the Ku80 protein expression in the same group was (11.03 ± 2.45) ％.The results of Real-time PCR showed that the telomere length of the cell group with recombinant plasmid (1.07 ± 0.07) was significantly shorter than that of the control group (4.42 ± 1.30,F =38.58,P ＜ 0.05) and that of the empty plasmid group (4.11 ±0.84,F =38.58,P ＜ 0.05).Compared to the control group,the telomere length of the empty plasmid group did not changed(4.42 ±0.84 vs.4.11 ±0.84).U2OS cells with Ku80 expression suppressed had lower SF2 than that of the control cells (F =1089.61,P ＜0.05),and resulted in the SER of 1.47.Conclusions The Ku80 inhibition cell model in telomerase-negative U2OS cell line is successfully established which has the shorter telomere length,and is more sensitive to radiation.Telomere shortening caused by pshRNA-of Ku80 is likely to be one of the mechanisms of radiosensitization in this kind of cell model.

The patient,a 51-year-old veterinarian,was hospitalized for a two-month history of painful oral ulcers without fever.Physical examination revealed two superficial ulcers in the palate.Laboratory examination showed a decrease in peripheral blood leucocytes as well as a hyperplasia of bone marrow.No abnormality was found by X-ray radiography or B-mode ultrasonography.The paileat tested negative for anti-HIV antibody.Classification Of CD+ cells showed that CD3+ cells amounted to 0.559,CD4+ cells 0.289,CD8+ cells 0.207,CD4:CD8 ratio 1.4,and all the parameters were in a normal range.Histopathological analysis of the ulcer tissue revealed a chronic inflammatory granuloma with clustered yeast-like cells.Fungal culture of the sample from ulcer tissue at 25℃on the slant of PDA yielded white filamentous colony;typical rudder-like macroconidias were observed with electronic microscopy following microculture;and the isolate showed a biphasic growth pattern.PCR was performed to amplify the internal transcribed spacer 1 (ITS1)and D1/D2 region of the isolated fungus followed by sequence analysis,and a homology of 97%and 99%was observed in the sequence of ITS1 and D1/D2 region,respectively,between the isolate and standard strain of Histoplasma capsulatum.A diagnosis of primary oral histoplasmosis Was made.The lesions subsided after 2-month treatment with oral itraconazole 400 mg per day.

OBJECTIVE To supervise the environmental fungal load and species distribution in transplantation department and intensive care unit of the Southwest Hospital,and to analyze the relationship with season,temperature,humidity,ventilation and personnel activities.METHODS Data from Dec 2005 to Jan 2006 were collected from liver transplantation department(LTD),cerebral surgery intensive care unit(CSICU) and central intensive care unit(CICU).Air,surfaces and tap water were sampled twice a month at each department.RESULTS The air fungal load was 123.63 CFU/m3,139.90 CFU/m3,7 CFU/m3 and 217.71 CFU/m3 at LTD,CSICU,CICU and outdoor,respectively.The five most prevalent fungi collected from air and surface were Penicillium spp,Cladosporium spp,Alternaria spp,Aspergillus spp and Saccharomyces spp in turn.The five most prevalent fungi collected from water were Saccharomyces spp,Candida spp,Aspergillus spp,Penicillium spp and Rhodotorula spp in turn.The fungal load in LTD was positively correlated with the average temperature and the average humidity;the fungal load in CSICU was correlated with the average temperature and the average humidity,but the correlation between air fungal load and personnel activities wasn′t observed.CONCLUSIONS It demonstrated the fungi are found in the environment of the hospital including air,surface and water.The air fungal load varies throughout the year.The crest-time is May to June and September to October.Air fungal load is lower in winter and higher in summer and autumn.The correlation between air fungal load and temperature and humidity is observed.

Objective To investigate the distribution of elderly metabolic syndrome(MS)and its components in the elderly population,and to analyze its correlation with the common chronic diseases in the elderly.Methods The general situation,history of diseases and health examination results were collected in 362 elderly with an average age of 79.2 years visiting Beijing Hospital.They were grouped into 3 groups of the young old age(65-74 years old),the middle old age(75-84 years old)and the older old age(85-98 years).The prevalence distribution of MS and its correlation with the common chronic diseases in the elderly were analyzed.Results MS prevalence in average was 28.4％ (103 cases)in the elderly population,with 18.6％ (67 cases) in the young old group,26.3％ (95 cases)in middle old group,and 33.7％ (122 cases)in older old group.The tendency was rising up with age increase,and the MS prevalence was higher in older old group than in other groups (x2 =8.57,P =0.03).Correlation was observed between genesis of MS and common geriatric chronic diseases.The prevalence differences in hypertension,prostatic hyperplasia,diabetes mellitus,and coronary heart disease were statistically significant higher in MS group than in non-MS group(P =0.02,0.01,0.02,0.03,respectively).Conclusions MS prevalence of elderly population is relatively high,and has significant correlation with other chronic diseases.We need to recognize the harm of MS,to conduct regular monitoring and management according to risk level,and practice patient educations to reduce MS negative effects on elderly life quality and health.