Objective To investigate the best pathologically diagnostic method for breast cancer before surgery. Methods Wet fine needle aspiration cytology was conducted in 68 cases of probable breast cancer by using a 10 ml syringe with size 7 needle. Results Malignant cells were found in 19 cases,without any false positive cases.Of the remaining 49 cases of negative cytological findings: 46 cases underwent segmental mastectomy,1 of which was postoperatively diagnosed as having fibrosarcoma by paraffin section examination and the rest of which were all demonstrated the presence of benign tumors;3 cases without surgical intervention were followed for (11～20) months and no evidences of breast cancer were found.The sensitivity was 95 0% (19/20),the specificity was 100% (48/48),the accuracy rate was 98 5% (67/68),and the false negative rate and false positive rate were 5 0% (1/20) and 0 (0/48),respectively.Of the 19 cases of positive cytological findings,no implantation metastasis in needle passage occurred.There were no complications in the study. Conclusions Wet fine needle aspiration cytology is safe,economical,convenient,quick,almost painless,and accurate,being the method of first choice in the diagnosis of breast cancer.

Aim To study the effect of Lacidophilus exopolysaccharides(LAEPS) on immunity. Methods Effects of LAEPS on immunity were investigated by delayed type hypersensitivity reaction, haemolytic plaque assay and macrophage function assay in mice. Results LAEPS ip 7～8 day promoted delayed type hypersensitivity reaction, increased haemolytic plaque and enhanced macrophage function in a dose-dependent manner.Conclusion LAEPS is able to enhances immunity.

Objective:To do factor analysis related to post-stro ke anxiety.Method:206 inpatients(male 126,female 80,mean age 63?16 )with stroke were tested with SAS,DNF,MMSE,LES and ADL.Results:T h e rate of anxiety after 1 month of stroke in our sample was 18.4%(38 cases).Mu lt i-factorial analysis showed,many factors including female,younger at onset,sever ity of stroke,poor general physical condition,less compliance with treatment,and other psycho-social factors(heavier family burden,less income,more life events, less social support)were all associated with anxiety after stroke.Conchus ion:Anxiety is a common complication after stroke,its occurrence were asso ciated with a variety of factors including severity of illness and psychosocial factors.

ObjectiveTo observe the effect of cerebellar fastigial nucleus treated by electrical stimulation on patients with acute cerebral infarction.Methods140 cerebral infarction patients with hemiplegia were randomly divided into the experimental group and control group with 70 cases in each group.The experimental group was treated with routine drugs and electrical stimulating on cerebellar fastigial nucleus;the control group was only treated with routine drugs.The changes of motor function of limbs and activities of daily living of two groups were evaluated after treatment.ResultsAfter 21 days treatment,the score of Fugl-Meyer assessment of the experimental group was 39.2±(7.7) and that of the control group was 26.3±8.1.There was a significant difference between two groups(t=4.22,P<0.01).The score of Barther index of the experimental group was 43.8±8.4 and that of the control group was 29.7±7.5.There was also a significant difference between two groups(t=4.97,P<0.01).ConclusionThe electrical stimulation on cerebellar fastigial nucleus is a new effective way to treat acute cerebral infarction.

OBJECTIVETo study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.

METHODEtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.

RESULTNine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.

CONCLUSIONAll compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.

Enzyme Inhibitors ; chemistry ; Glycoside Hydrolase Inhibitors ; Lythraceae ; chemistry