Aim To study the effect of Lacidophilus exopolysaccharides(LAEPS) on immunity. Methods Effects of LAEPS on immunity were investigated by delayed type hypersensitivity reaction, haemolytic plaque assay and macrophage function assay in mice. Results LAEPS ip 7～8 day promoted delayed type hypersensitivity reaction, increased haemolytic plaque and enhanced macrophage function in a dose-dependent manner.Conclusion LAEPS is able to enhances immunity.

Objective To explore the application of the virtual private network(VPN) to picture archiving and communication system(PACS) for the remote access and management of PACS.Methods The client-ports(namely the remote computers) were connected to Internet by broadband such as ADSL,and the web server of PACS was connected to Internet by broadband router with VPN function so that the remote computers could access the web server of PACS remotely.By the VPN technology,the system administrators of PACS could diagnose and maintain the general malfunctions of the radiology's local network,and thus the remote management came true.Results The remote access and management of PACS had been available in our hospital for half a year since the application of the VPN technology in PACS.By more convenient medical services the work efficiency had been greatly improved.Conclusion The application of VPN technology to PACS,which is characterized by low expense,dependable performance,simple maintenance,can realize remote access and management of PACS.

Objective To investigate the relationship of insulin resistance and secretion during late pregnancy in women with glucose intolerance.Methods Immunoenzymetric assay was used to measure the fasting serum insulin levels in 122 pregnant women which including of 36 pregnant women with gestational diabetes mellitus(GDM),34 pregnant women with gestational impaired glucose tolerance(GIGT),and 52 pregnant women with normal glucose tolerance(NGT).The fasting plasma glucose levels were measured by glucose oxidase method.The insulin sensitivity index(ISI) and islet secretive function index(IFI) were compared between the three groups.Results ISI had an increasing trend from NGT group,GIGT group to GDM group(P

OBJECTIVETo study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.

METHODEtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.

RESULTNine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.

CONCLUSIONAll compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.

Objective To investigate the influence of leptin receptor (LEPR) gene K109R polymorphism on the risk of nonalcoholic fatty liver disease (NAFLD) and its interaction with PNPLA3 I148M polymorphism in the Han Chinese population in Qingdao,China.Methods Blood samples were collected from 296 NAFLD patients and 321 healthy controls,and the genotypes of these patients were determined by PCR and genotyping.Related statistical analyses were performed to compare genotypes,alleles,and clinical data between the two groups.Generalized multifactor dimensionality reduction (GMDR) was used to investigate the interaction between LEPR K109R and PNPLA3 I148M genes.Results The distribution of LEPR K109R genotypes and alleles showed no significant differences between the NAFLD group and the control group (P ＞ 0.05).PNPLA3 I148M gene polymorphisms were closely associated with the risk of NAFLD,and the risk of NAFLD in G mutant gene carriers was 2.07 times that in patients who did not carry this gene (OR =2.07,95％ CI 1.423-3.013,P ＜ 0.001).The joint action of LEPR K 109R and PNPLA3 I 148M significantly increased the risk of NAFL (OR =3.393,95％ CI 1.856-6.201,P ＜ 0.001).Conclusion In the Han Chinese population in Qingdao,LEPR K109R gene polymorphism is not associated with the risk of NAFLD,but its interaction with PNPLA3 I148M polymorphism can significantly increase the risk of NAFLD.