Objective To evaluate the effect of double interventional thrombolysis for treatment of acute venous thrombosis in lower limb and discuss the problems of its clinical use. Methods 24 patients with acute venous occlusive disease associated with thrombus formation were treated by double interventional thrombolysis including 17 cases of femoral venous approach and 7 with femoral artery catheterization. The total dosage of urokinase was from 2 500 000 U to 5 000 000 U. Results All together 24 cases were undengone this proceduce; outcoming with complete thrombolysis in18(75%), partial and noneffective in 3(12.5%) and 3 (12.5%) respectively. The total effective rate was 87.5%. Conclusions Double interventional thrombolysis is highly effective to the patients with acute venous thrombosis.

Objective. In order to investigate 5,10-methylenetetrahydrofolate reductase (MTHFR)s polymorphic changes in psoriasis vulgaris.Methods.We detected mutation of site C677V of MTHFR in 39 psoriastics by PCR-RFLP.Results.Genotype frequencies of the psoriastics were C/C＝20.15%,C/T＝48.72% and T/T＝30.77%; the allelic frequencies were C＝0.4487 and T＝0.5513. Homozygous mutant (TT) of the psoriastics was significantly different from the normal control group by X2 test.Conclusion.C677V mutant of MTHFR might be related with psoriasis.

Fournier gangrene is a relatively rare clinical critical disease, and its clinical symptoms are not specific and easily unrecognized by some clinicians. It has the features of acute onset, quick development, severe illness, and often accompanied by infection shock which is seriously life-threatening. It is difficult in treatment with high medical costs and long length of hospitalization, which increases pain for patients and relatives and brings heavy economic and psychological burden on patients, society, and medical workers. By reviewing the literature home and abroad and combined with clinical practice, I summarize the researches on concept, epidemiology, clinical manifestation, diagnosis and treatment of Fournier gangrene, in order to provide reference for vast number of clinical workers.

OBJECTIVETo investigate common chromosomal changes and the LOH frequency of microsatellite loci in primary gastric cancer samples in order to locate the deleted regions in which human gastric cancer related genes might exist.

METHODSComparative genomic hybridization (CGH) was used to define global chromosomal aberrations in 43 primary gastric tumors. Based on the results of CGH, analysis of loss of heterozygosity (LOH) was performed in chromosome 19 in which the loss was first discovered in the gastric cancers. The PCR-based approach was used to investigate 22 loci, which are spaced at 1.1 - 10.9 cM intervals throughout chromosome 19. The amplified PCR fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan trade mark and Genotyper trade mark.

RESULTSCGH analysis revealed gains in chromosome 3p (8/43), 8q (8/43), 20 [20 (9/43), 20p (7/43), 20q (4/43)], 12q (16/43), 13q (12/43) and losses in 19 [19 (15/43)], 7 [17 (8/43), 17p (10/43)], 16 (10/43) and 1p (11/43). Among the 43 evaluated samples, the most frequent LOH was detected at locus D19S571 (27.81%).

CONCLUSIONSThe tumorigenesis of gastric cancer includes several chromosomal changes. The aberration of chromosome 19 was the first common change founded in gastric cancer. The region near the D19S571 might harbor potential genes related to the tumorigenesis of gastric cancer.

Chromosomes, Human, Pair 19 ; Humans ; Loss of Heterozygosity ; Nucleic Acid Hybridization ; Peutz-Jeghers Syndrome ; genetics ; Stomach Neoplasms ; genetics