Objective To investigate the relationship between the serum c oncentration of TGF-? 1 and invasion and metastasis of gastric cancer,and the effect of surgical treatment on the serum concentration of TGF-? 1.M ethods The serum concentration of TGF-? 1 of 48 cases of gastric can cer was detected by ELISA 3 days before and 7-10 days after surgical treatment. Results The serum concentration of TGF-? 1 of gastric cancer before surgical treatment (404.6? 12.0) mg/L was higher than that after surg ical treatment (294.3?33.9) mg/L (P

186 serum samples from 81 patients with gastric cancer were determined for circulatingimmune complexe (CIC) by using the polyethylene glycol turbidity assay. Compared with 105healthy blood donors, the mean value of CIC were significantly increased in pretreatment pati-ents (47.46?24.52vs.27.67?12.09), and the corresponding figures for stage Ⅰ+Ⅱ, Ⅲ and stage Ⅳpatients were 38.60?14.24, 42.04?13. 18 and 54.31?29. 15 respectively. The incidence of positivityof CIC in pretreatment patients was 32.1%. The mean value of CIC is significantlyhigher in patients whose disease could not be treated with radical surgery than in patientswhose disease could.CIC levels increased significantly during 11～45 days after radical operation.CIC levels were positive correlated with levels of serum IgA, IgM, C_3, and negative corre-lated with the rate of lymphocyte transformation. The patients found positive for ANA hadhigher incidence of positivity of CIC than those found negative for ANA.The clinical signifi-cance of CIC in patients with gastric cancer was also discussed.

Objective:To investigate Helicobactor pylori (H. pylori) infection status and interfamilial transmission pattern in Zhengzhou area. Methods:A cross-sectional study was conducted from September 2020 to march 2021, among 731 individual from 266 families randomly selected from 9 communities of Zhengzhou area. H. pylori infection status was determined by serum antibody tests, and 13C-urea breath test was performed in the previously eradicated population to clarify the current infection status. The individual and familial infection rate, infection status for couples and children and adolescent were analyzed. Results:Among 731 individuals from 266 families, 397 of them were H. pylori positive. The individual infection rate was 54.31% (397/731); among infected individuals 77.83% (307/397) were infected with type Ⅰ strain, 22.67% (90/397) were infected by type Ⅱ strain. Annual household income ( χ2=0.419, 0.410, 0.213, all P>0.05), smoking history (χ 2=0.071, P>0.05), drinking history ( χ2=0.071, P>0.05), dining place ( χ2=0.009, P>0.05), gastrointestinal symptoms ( χ2=0.047, P>0.05), family history of gastric disease ( χ2=0.069, P>0.05), and history of gastric cancer ( χ2=0.004, P>0.05) had no significant differences between H. pylori-positive and -negative groups, but the infection rate in individuals with higher education level was lower ( χ2=4.449, P<0.05). The infection rate was significantly higher in≥18 age groups compared with<18 age groups ( χ2=6.531, 23.362, 20.671, 24.244, 37.948, 14.597 and 5.170, all P<0.05). The familial H. pylori infection rate was 87.59% (233/266), and in 61 families all member were infected (26.18%, 61/233). The positive rate was 23.08% (6/26) in 50 families with children under 18 years when both parents were infected. Among 231 coupled families, both couples were infected in 78 families (33.76%), one couple was infected in 113 families (48.92%), and both couples were not infected in 40 (17.32%). With the increase of marriage time, the infection rate of both spouses increased significantly ( χ2=7.775, 12.662, 15.487, all P<0.05). Conclusions:The distribution of H. pylori infection presents a family cluster pattern, and intrafamilial infection is an important transmission rout of H. pylori. The type I strain of H. pylori is the dominate strain in this area.

Objective:To analyze the sequence characteristics，chromosomal location，gene structure，conserved motifs，phylogenetic evolution and differential gene expressions of the Cannabis sativa YABBY transcription factor family，in order to provide a molecular basis for in-depth study of YABBY gene function and theoretical support for the selection and breeding of superior hemp varieties. Method:The bio-informatics method was used to identify and analyze the CsYABBY gene family of the original hemp seed plant. PlantTFDB，ExPASy，MEME，CELLO，PLANTCARE and other online websites and TBtools，MEGA，DNAMAN and other software were used for prediction，visualization and analysis. Result:C. sativa contains 6 YABBY gene members distributed on 5 chromosomes，in which 5 members are localized in the nucleus and 1 in extracellular， they consist of 185-235 amino acids， and the isoelectric point is between 5.05 and 9.34， the molecular weight is between 20 582.45-26 282.7 Da. All of CsYABBY proteins contain two conserved domains， namely Zinc finger domain and YABBY domain. CsYABBY genes have multiple cis-acting elements，and their expressions differ in different tissues and cultivars. Conclusion:The expressions of CsYABBY may be affected by hormones and externally environmental factors. CsYABBY gene expressions are tissue-specific. In addition，YABBY transcription factor family may play an important role in regulating the development of C. sativa female flowers，and subfamilies YAB1 and YAB5 may be involved in the synthesis of cannabinoids.