Introduction Currently nations throughout the world are studying and disseminating their traditional medicine and Mongolians create new trend and attitude of medical care and service besides resuming their great heritage and culture of traditional medicine. World Health Organization recommends countries to develop their traditional medicine linking with modern science of medicine, based on particular surveys and analyses are conducted. However, the basic concept for developing Mongolian traditional medicine have been developed and implemented twice, “Government policy on Mongolian traditional medicine”, studies plan of united theory of traditional and modern medicines in master plan for developing Mongolian science and technology studies plan, “Master Plan for health sector” – the policy renovation for 2006-2015, “The frame of implementing master plan for health sector” (2006 2010) and other documents have been developing and implementing since 1990s but lack of studies on whether effective influences of traditional medicines to which stages and to which clinical symptom of dominated diseases among population and whether the traditional medical care meets with customers’ demand are considered as burning issues in determining the further development of traditional medicine. As well as, there are lots of issues that have not been studied and determined yet as diagnosis of traditional medicine, clinical and preventive guidelines and standards have not been developed, have not been involved in united system of health information, structural and operational management of institutions that hold traditional medical care and demands of medical care and professional staffs of traditional medicine. Aim of the study: To study and evaluate the current situation of Mongolian traditional medicine as well as determine trends in future. Purposes of the study: 1. To study the historical periods of the development of Mongolian traditional medicine that have been 01/01 developed since 1921 when health sector initiated in Mongolia. 2. To study Mongolian traditional medical service service by health care levels. 3. To study and to determine the human resource demand and capacity of medical care of traditional medicine. 4. To develop the management model of showing medical care of traditional medicine and health systems based on coherences of western and oriental medicines. Materials and methods This study observed the population criteria of patients who were treated in hospitals, hospital service levels, methods of diagnosis and treatment, common diagnose causes, types of treatment and the results of treatment in order to study the implementation of hospital service of Mongolian Traditional Medicine. The study was conducted in 3 different levels of hospitals: as a primary level hospital Umnudelger soum hospital, Khentii aimag /province/ was chosen, as a secondary level hospital we selected Center for Traditional Medicine of Khentii province, as a tertiary level hospital we chose the clinical hospital of Traditional Medicine, Science, Technology and Production Corporation (CHTMCST). We investigated aid and service information of Traditional Medicine obtained from 952 histories of patients who were treated in hospitals in 2006 and 2007. Analysis was made on policy documents by using analyzing and integrating related document. The study data were developed by making descriptive analysis on digital information, raising related hypothesis, checking it with statistical tests and processing in results. Research data was processed by Microsoft Excel 2007, statistical data was performed by SPSS-12.0 program and descriptive analysis was performed in quantitative information. Result By concluding the activities organized and implemented nationally on the field of traditional medicine for the period since 1921 when health system in Mongolia initiated, it could be considered to divide into stages as, beginning period of developing Mongolian traditional medicine in coexistence with modern health science (1921-1930), deprived period of Mongolian traditional medicine from national service (1930-1958), restoring period of developing Mongolian traditional medicine (1959-1999) and the ensured period of Mongolian traditional medicine as a composition of health science system (1999 up now). It was determined that the percentage of female patients is more than twice (2.3) higher (63.7%) in contrast with male patients (30.1) in all three-level hospital aid. It means in each level of three level hospitals female patients were more in number. Surprisingly, 80.9% of the patients’ age was over 35 and from them 44.7% was at their pension age. This age group is rapidly predominant compared with others. In chosen hospitals patients with diseases and disorders of digestive, urinary-genital, nervous and blood circulation systems are cured commonly. In three-level hospitals examining patients using methods of traditional medicine was differed (p=0.01). In doubled number, 401 or 42.1% of doctors combines both modern and traditional methods of treatment (95% trust limit 42.1+0.017). We investigated if types of treatment depend on the level of hospitals in urban and rural areas by x 2 criteria and concluded that it had statistical significance or the percentage of treatment types went up if the level increased (p

BACKGROUND Bovine colostrums is the milk secreted by cows during the first few days after parturition. It contains many essential nutrients and bioactive components, including growth factors, immunoglobulins, lactoperoxidase, lactoferrin and cytokines ets. Lactoferrin has been reported for its multifunctional properties such as antifungal, antibacterial, antiviral antioxidant and anticancer activities. The aims of this study focused on the isolation and purification of lactoferrin from Mongolian bovine colostrums. Lactoferrin purified using HiTrap DEAE an ion exchange chromatography. Lactoferrin purification efficiency was about 60.5%. The single band of purified lactoferrin has been observed in SDS-PAGE electrophoresis. METHODS Bovine colostrum was collected at a cow farm in the Darkhan province of Mongolia. At first the cream was separated by centrifugation (10000 xg 20 min at 4oC). In order to separate the whey, the samples were precipitated with 1mol/l to pH 4.6 and centrifuged at 10000 g 20 min again. The samples of whey were stored at -18oC to the analysis. Lactoferrin was purified by HiTrap DEAE an ion exchange chromatography using 0.005 M phosphate buffer (pH 7.7) and linear gradient NaCl from 0.25M, 0.5M, 1M. During chromatography, protein in the eluents was monitored by ultraviolet absorbation at 280 nm with the instrument. Purity test done by using polyacrylamide gel electrophoresis under denaturated condition (SDS-PAGE) method by Laemmli (1970). For HPLC determination of the lactoferrin by Shimadzu Nexera X2 HPLC system with UV/ VIS detector were used. Detection was carried out at the wavelength 280 nm. Separation was performed on a chromatographic column Protein R C18 ,2.2 x 150 mm, 5 μm particle size. Linear gradient and flow rate 0.2 ml/min were used. Mobile phase a consisted of water / acetonitrile/ trifluoroacetic acid ( 95:5:0.1). The column temperature was set at 40oC and injection volume was 10 μl. Data were collected and evaluated by software Lab Solution. An external standard method for quantification analytes was used. RESULTS Purified lactoferrin in the present study had a good concentration and purification efficiency was about 60.5 %. Protein fraction from 1M NaCl gradient delivers sharp and clean peak to HPLC chromatogram that fits intensity and retention time of standard bovine lactoferrin. Ammount of lactoferrin in bovine colostrums was 0.6 mg/ml and it`s molecular weight 80 kDa as a standard sample. The retention time of lactoferrin fraction which is purified by SDS-PAGE gel electrophoresis. The peak of fraction same compared to the standard lactoferrin 5.8 minutes by HPLC analysis. CONCLUSION Ion exchange chromatography shows reliable and easy isolation of lactoferrin from Mongol bovine colostrum.