Cytokine-induced killer cells (CIK cells) are a group of CD3+ CD56+ T cell-based heterogeneous cells generated in vitro,with T-cell receptor (TCR) specificity of CD8+ T cells and anti-tumor activity of non-restrictive major histocompatibility complex (MHC).Recent studies indicated that vaccination with CIK cells induced strong anti-tumor activity in the treatment of malignant lymphoma.These researches show that CIK cell-based immunotherapy has the important values for patients with malignant lymphoma.

BACKGROUND:The major aim of repair of lumbar spondylolisthesis is to restore and reconstruct the stability of spine sequence, and to relieve compression of herniated disc on nerve root. Pedicle screw system and bone graft fusion become the preferred way to treat spondylolisthesis of most scholars. Most scholars are prone to posterior interbody fusion or posterolateral interbody fusion.
OBJECTIVE:To compare the clinical curative effects of posterior autologous iliac bone graft fusion and posterior Cage intervertebral bone graft fusion in patients with degenerative lumbar spondylolisthesis by observing fusion rate and height of intervertebral loss.
METHODS:A total of 61 patients with degenerative lumbar spondylolisthesis in Xuancheng Central Hospital from July 2008 to December 2013 were enrol ed in this study. According to the different types of interbody fusion, 37 cases in autologous ilium group received posterior autologous iliac bone graft fusion. 24 cases in the Cage interbody fusion cage group underwent posterior Cage interbody fusion cage fusion. Operation time, blood loss, spondylolisthesis reduction, graft fusion and Japanese Orthopaedic Association score were compared between 
the two groups. The significance of different fusion manners on prognosis was explored in patients with degenerative lumbar spondylolisthesis.
RESULTS AND CONCLUSION:No significant difference in blood loss was detected between the two groups (P>0.05). However, the operation time was significantly longer in the autologous ilium group than in the Cage interbody fusion cage group (P<0.05). The reduction rate, bone graft fusion rate and improvement rate of clinical curative effects were good in both groups, and no significant difference was detectable in above indexes between the two groups (P>0.05). Significant difference in the height of intervertebral loss was detectable between the two groups in final fol ow-up (P<0.05). The height of intervertebral loss was better in the Cage interbody fusion cage group than in the autologous ilium group. These findings indicate that two kinds of bone graft fusion manners for lumbar spondylolisthesis obtained good reduction and clinical curative effects, but the height of intervertebral loss was smal in the Cage interbody fusion cage fusion during long-period fol ow-up, and the clinical effect was good.

Objective To investigate the clinical characteristics of Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡ.Methods The clinical data of 1 case Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡwas retrospectively analyzed.Results The patient presented muscle weakness in proximal limbs, fasciculation, bulbar palsy, amyotrophy, postural tremor, sensory disturbance, gynaecomastia, impotency.The level of serum creatine kinase and urinary glutaric acid were elevated. Electromyogram and nerve and muscle biopsy supported mixed damage in peripheral nerves, as well as neurogenic and secondary myogenic pathologic changes in muscles.Gene sequencing indicated trinucleotide CAG repeated amplification for 47 times in chromosome X.Diabetes was diagnosed at 7 years after onset.Conclusions The pathogenesis of Kennedy disease is very complex, which may involve myogenic factor.The treatment to the secondary mitochondrial and lipid metabolic disturbance in muscle can improve the muscle weakness to a certain extent.

Objective: To observe the clinical effects of scalp acupuncture and body acupuncture on post-stroke ataxia. Methods: 124 cases of post-stroke ataxia were treated with scalp and body acupuncture to observe the changes of blood velocity in the blood vessels of the brain and the total effects after treatment. Results: The total effective rate was 93.5% based on the result of Berg Balance scale, and was 91.1% on the activities of daily living (ADL) index. The blood velocity of the vertebrobasilar artery was higher after the treatment compared to before treatment (P＜0.05). Conclusion: Acupuncture had good clinical effects.

Metastasis-associated in colon cancer-1 (MACC1) is a recently discovered gene associated with colon cancer metastasis,there is significant relationship indicated from some studies between MACC1 and different malignant tumors.It may play an important role in the regulation of tumors metastasis.This article reviewed the expression and regulating function of MACC1 in different cancers including colorectal cancer,hepatic cancer,gastric cancer,lung cancer,ovarian cancer,breast cancer,and so on.It may offer clues to find a new target for target treatment of cancer metastasis.

Objective The abnormal proliferation of renal cells underlies the pathology of progressive glomerulosclerosis.Cell proliferation is regulated by cell regulatory proteins.This study aims to investigate the expression of cyclin-dependent kinase inhibitor P16INK4a in renal tissue of patients with primary mesangial proliferative glomemlonephritis(MsPGN)and its clinical significance.Methods Paraffinembedded renal biopsy renal tissue sections from 36 patients with MsPGN were detected by immunohistochemical staining.Normal renal ti8.Sue sections from 6 nephrectomized patients with trauma were used as controls.Possible correlation between p16 positive area and sclerotic and crescentic glomeruli,blood pressure,serum creatinine,endogenous creatinine clearance rate,and total proteinuria were evaluated.Resuits There were very few expression of P16INK4a in normal glomemli and renal tubular-interstitial.Compared to the normal comrol group,the expression of P16INK4a in 36 renal biopsy specimens with MsPG'N was significantly higher(P<0.01),especially in sclerotic and crescentic glomeruli,and it had wild expression of the same components in no sclerotic and crescentic glomeruli.There was a statistic difference(P<0.01).No statistic difference was found between the expression of P16 INK4a in renal tubular-interstitial of two groups(P>0.05).Therewag positive correlation among P16 INK4a of glomeruli without use of glucocorticoid/immunoinlfibitors and ACEI/ARB(r=0.774,0.497,P<0.01),also blood pressure and 8ul～m creatinine(r=0.64,0.473,P<0.01).It was negstively correlated with endogenous creatinine clearance rate and total proteinuria(r=-0.487,-0.694,P<0.01).There is no correlation between P16INK4s and clinical data in renal tubular-interstitial.Conclusion This study demonstrated that overexpression of P16INK4a in renal tissue with MsPGN may promote the regression ofabnormal proliferation and senescence cells,and induce sclerotic and crescentic glomeruli.The expression level of P16 INK4a not only reflected the degree of renal function,but also hypertension and proteinuria.In brief,expression of P16INK4a may be an important marker in renal sclerotic or crescentic glomeruli of MsPGN.Some drugs may prevent the expression of P16INKa.

At present,pharmacy experiment is mainly in accordance with the traditional teaching mode,and students mechanically complete the experiments according to the demonstration of teachers.There is lack of interaction between teachers and students,and students lack independent thinking and experimental content innovation,which lead to the inefficient teaching effects.By flipping the classroom in the pharmacy experimental teaching,teachers prepare teaching courseware,teaching video and learning tasks before class,in class emphasize the experiment key and the difficult point,and carry on the preliminary experiment,and after class,summarize and analyze the practical problems that students have in the experiment,which have effectively aroused students' professional interest in learning and promoted their practical operation and independent thinking ability,and achieved good teaching results.

Objective To explore the multiple pelvic fractures projection methods and value. Methods 50 cases of pelvic trauma patients were divided into two groups for control photography, and underwent X-ray examination with different projection angles. Results The display effect of the image of the image by 35? projection was better than that of the control group. Conclusion 35? projection results in the images satisfying clinical requirements.

Objective To investigate the application of computed radiography(CR) in X-ray projection of whole lower limbs.Methods The radiography in the patient with bilateral knee-malformation was performed using 500 mA X-ray apparatus and several imaging plates(IP) jointed together,then the images including whole both sides of lower limbs were obtained by post-proccesing technique.Results The images not only could show the whole lower limbs on a single film,but be intact.The images also could be used for measuring the length of leg,the angle between the axises of the thigh and the magnifying really.Conclusion The image of whole bilateral lower limbs with a good quality and unity can be acquired using CR and post-processing technique appropriately.

Background and purpose:Brain is one of the most common sites for distant metastasis in patients with non-small cell lung cancer,and the prognosis of patients with brain metastasis is usually dismal.The purpose of this retrospective study is to document the relationship between the prognosis of lung cancer patients with brain metastasis and the expressions of p53,nm23 and VEGF in resected lung cancer tissues.Methods:Ninety-two patients who were definitively treated with surgery for non-small cell lung cancer but lately developed brain metastasis between 1997 and 2005 were identified in our institution.Their clinical data were retrieved and retrospectively reviewed.All pathological specimens of their resected lung cancer were examined for the expressions of p53,nm23 and VEGF by immunohistochemical staining.The association between the treatment outcome and the expression of the above mentioned biomarkers were analyzed.Results:The median survival time(MST) of patients with p53(+) versus p53(-) was 11.0 versus 11.9 month,respectively.The 1,2,and 3-year overall survival rates were 45.71%,22.86%,and 18.29% respectively for p53(+) patients,and 49.55%,16.12%,and 8.89% respectively for p53(-) patients(P=0.5179);The MST of patients with nm23(+) versus nm23(-) was 13.0 versus 10.1 month,respectively.The 1,2,and 3-year overall survival rates were 54.20%,21.51%,and 16.45% respectively for nm23(+) patients,and 32.0%,12.0%,and 4.0% respectively for nm23(-) patients(P=0.1075);The MST of patients with VEGF(+) versus VEGF(-) was 10.5 versus 12.2 months,respectively.The 1,2,and 3 year overall survival rates were 42.20%,0,and 0 respectively for VEGF(+) patients,and 50.0%,25.41%,and 16.57% respectively for VEGF(-) patients(P=0.0231).Conclusions:VEGF was a significant adverse prognostic factor for patients with non-small cell lung cancer who developed brain metastasis.Lung cancer patients whose tumor tissue demonstrated positive VEGF expression had reduced overall survival rates at 1,2,and 3 years after surgery.The expression of p53 and nm23 are not significantly associated to the prognosis of this group of patients.