Neurodegenerative diseases,which are common nervous system diseases,seriously affect people''s life quality and even lives. So the pathogenesis of neurodegenerative diseases is attracting more and more attention in recent years. People gradually realize that endoplasmic reticulum stress is associated with the pathogenesis of many diseases through studying the function of the endoplasmic reticulum. Neurodegen-erative diseases, endoplasmic reticulum stress,unfolded protein responsewere used as the key words of retrieval performance in the databases such as Pubmed,CNKI and so on. And the papers which closely re-lated with the theme were chosen to investigate the association between alzheimer''s disease,parkinson''s dis-ease,Huntington'' s disease ,amyotrophic lateral sclerosis and ERS. It turned out that the expression of mo-lecular chaperone GRP78/Bip was increased in all neurodegenerative diseases, and the phosphorylation of eIF2α increased in most neurodegenerative diseases. However, the expression of some ERS-related factors was completely different in different neurodegenerative diseases. Thus,the study of ERS may be an important breakthrough for the pathogenesis and treatment of neurodegenerative diseases.

OBJECTIVE To establish hospital infection information surveillance system connecting with hospital information system(HIS)in order to achieve real time monitoring,process management and data upload for hospital infection.METHODS According to the national rules of law and the experience of hospital infection management,the software had been developed by using PowerBuilder as program tool and Oracle as database.RESULTS The software could be utilized in the real time monitoring for all inpatients.It had the function of inquiry,statistics and upload.CONCLUSIONS The subsystem connecting with HIS may improve efficiency and effectiveness for preventing and controlling hospital infection.

Flipping the classroom is a new teaching mode in which the students learn some-thing through the network autonomous learning before class, in classroom students interact with teach-ers, internalized and absorb what they have learned, and then consolidate it after class. So it can resultin real-timeevaluation of learning effect by different means, different objects and form and can ef-fectively give secondary feed back comments, which has important significance for the improvement of teaching and learning effects at all levels. Article discusses how to effectively use formative assess-ment method to conduct effective monit oringand feed back to improve the flipped classroom activi-ties. It also explore show to strengthen and exercise their student's ability in the application process.

Objective To discuss the clinical feature and the treatment of severe hand, foot and mouth disease (HFMD), and provide the basis for control of the disease. Methods The clinical data of 60 children with severe HFMD were retrospectively analyzed. Results All the 60 cases appeared fever and the erythra in hand, foot, mouth and gluteal region. Part of the children appeared jumping, body shaking, poor spirit and/or sleepiness. Some children appeared convulsion, and neurogenic pulmonary edema, pulmonary hemorrhage, cardiorespiratory failure happened in 2 critical severe cases. The children were given the comprehensive treatment including ribavirin, mannitol, human immunoglobulin and methylprednisolone. Forty-three cases were cured, 15 cases were improved, and 2 cases died. Conclusions Severe HFMD children usually appear critical condition. Early detection of critical signs and correct and effective clinical treatment can promote children's recovery and reduce the mortality rate.

Objective To establish mouse models of total body irradiation (TBI) with different doses of 60 Co radiation combined with skin defect, so as to investigate the influence of TBI on wound healing and the pathophysiological changes in combined radiation injury and skin defect. Methods A total of 180 female Kunming mice were irradiated with a single dose of 4, 6 or 8 Gy 60 Co ray. Within 30 min after irradiation,a full thickness square wounds (1.5cm×1.5cm) was made on the back of mice to establish animal models of TBI combined with skin defect (n=50), another 30 mice with pure skin defect were used as controls (n=30). Mice in each group were sacrificed on the 3rd, 5th, 7th, 10th and 14th day after injury, each time 6 mice, and the full thickness wounds were harvested. Histological method was used to evaluate the changes of inflammatory cells, fibro blasts and new blood capillaries in the wounds. Image analysis system was used to analyze the areas of the residual wounds. The survival rates and body weight changes of mice within 14 days were analyzed in all groups. Results On the 7th and 14th day after injury, the survival rates of mice in the 6 Gy group were 75% and 55%, respectively. While in the 8 Gy group the survival rate of mice was only 33% on the 7th day,and all the animals died by the 10th day. Within 14 days after injury, the body weight loss of mice demonstrated an increasing tendency with the increase of radiation doses (4,6,and8 Gy groups). TBI delayed wound healing in mice with the increase of irradiation dose.The unhealed areas in the 6 and 8 Gy groups were larger than that in the control group on the 2nd day (P<0.01), and that in 4 Gy group was significantly larger than that in the control group on the 8th day (P<0.05). H-E staining showed that the early inflammatory responses were inhibited, the increase of fibroblasts and new capillaries were greatly delayed, and the granulation tissue formation and reepithelialization were slowed down in the TBI group compared with the simple wound group.Conclusion Animal models of TBI with 6 Gy 60 Co ray combined with skin defect can serve as a platform to study the mechanism of difficult healing and early treatment of radiation injury combined with skin defect.

Objective To explore the impact of partial or complete rupture of the tibiofibular syndesmosis on the biomechanical properties of the ankle joint.Methods Six fresh cadaveric specimens of adult ankle were used for creation of 3 models of tibiofibular syndesmosis.In model A,the medial and lateral ligaments of the ankle joint and the tibiofihular syndesmosis were intact.In model B,only the anterior lower tibiofibular ligament was broken.In model C,all inferior tibiofibular ligaments were broken.The 3 models were tested with an electronic pressure sensor in a BOSE material testing machine respectively at 5 positions:10° dorsal extension,20° inversion,20° eversion,20° plantar flexion,and neutral position.The peak values and locations of the compressive stresses of the 3 models at 5 positions were recorded.All the data were collected and analyzed using IBM SPSS 22.0 statistical software.Results Model A exhibited the smallest average stress at all the 5 positions,followed by model B and model C,with significant differences between groups (P ＜ 0.05).The locations of main compressive stress at the neutral position,10° dorsal extension,and 20° plantar flexion in model B and model C tended to expand outwards compared with model A,especially in model C.Conclusions Rupture of the anterior lower tibiofibular ligament and inferior tibiofibular ligaments may increase the compressive stress on the ankle joint,especially rupture of the inferior tibiofibular ligaments.The location of main compressive stress tends to expand outwards when the anterior lower tibiofibular ligament and inferior tibiofihular ligaments are broken,especially the inferior tibiofibular ligaments.

Objective:Performance appraisal of large-scale medical equipment has a significant impact on hospital management. Process equipment performance evaluation of digital is an important part of digital medical&hospital. How to fully utilize the large number of advanced medical equipment resources, how to monitor and manage equipment are focuses that hospital administrators pay much attention to. Methods: The effective performance appraisal of large-scale medical equipment can promote the resource allocation optimization, accurate and scientific management, reasonable and benign application, and then allows large-scale medical equipment fully and effectively applied to clinical practice. Results: Collecting basic data is the basis for performance appraisal of large-scale medical equipment; its instant validity, accuracy, and scientific can enhance the operability and the scientific accuracy of the performance appraisal for large-scale medical equipment. Conclusion:The acquisition of basic data has few specialized research in previous studies, the rules set up for basic data acquisition of large-scale medical equipment in this paper can improve the timeless, accuracy and normalization of the basic data using in the performance appraisal of large-scale medical equipment.

OBJECTIVE: To provide reference for the management of central pharmacy in our hospital. METHODS: In daily work, performance score of a job was related with quality evaluation using grouping work model and management method of allocating a specific responsibility to a specific person. RESULT & CONCLUSION: The work process of central pharmacy is clear and plays a great role in economizing on resources relatively.

Whether three genes [?-aminolevulinic acid dehydratase (ALAD) gene, Vitamin D receptor (VDR) gene and hemochromatosis gene] can make certain individuals more vulnerable to lead are discussed. Polymorphisms of ALAD gene have been associated with the accumulation and distribution of lead in the blood, bone and internal organs in humans and animals. VDR gene has been implicated in the control of calcitriol levels in serum, which normally regulates calcium absorption and can in turn affect lead levels. Hemochromatosis gene, associated with a disease that leads to excessive iron accumulation, may also influence the absorption of lead.