Introduction.M.Colombo, W.Lange studies showed that 30-40% of people became chronic after suffering fromHepatitis B and C virus, about 50% of chronic cases transformed into primary liver cancer. There arefew studies in our country were conducted on hepatitis among healthcare professionals, particularnursing personnel.Goal.To identify antigens and antibodies of hepatitis B and C virus among nursesMaterials and Method.We carried out cross-sectional study among selected nurses, to determine surface antigen of hepatitisB virus and antibodies to hepatitis C virus. For identification of these antibodies and antigen, andvalidation of results Serodia tests from Fujinebo Company (Japan) and Beringnost (Germany) wereused respectively.Results.There were 598 nurses from the State Central Clinical Hospital, Shastin’s State Hospital, Hospitalof Military of Justice and Internal Affairs, and the National Center of Maternal and Child Health, whoparticipated in the study. From 5 hospitals a 598 nurses surveyed and revealed the hepatitis B virussurface antigen positive 18.9%, hepatitis C virus antibodies in 23.2%, B and C viruses detected by1.2% combined.Conclusion.The study identified that 43.2 percent of nurses surveyed on hepatitis B and C viruses were detected;it shows a high prevalence among the nurses. There is an urgent need to provide knowledge tomedical personnel regarding standards during procedures, concerning hepatitis infections, monitoringand improve technology used during procedures.

BACKGROUND. The 84.4 percent of newly diagnosed patients with diabetes have obesity in Mongolia. Nowadays, prevalence of obesity has increased steadily in Mongolia. Diabetic patients with viral hepatitis has high risk of having liver cirrhosis. Therefore, screening of fatty liver and liver fibrosis in diabetic patients is more important. The main diagnosing method of fatty liver and liver fibrosis is liver biopsy and histology but so far, we are able to detect viral infection using viral marker and determine fibrosis stage of NAFLD in patients who has diabetes mellitus type 2. Using noninvasive method determining liver fibrosis involve many researches to reveal new biomarkers and technics to find out liver fibrosis. Japanese researchers has found The Wisteria floribunda agglutinin- positive human Mac-2-binding protein (WFA+-M2BP) was recently shown to be a liver fibrosis glycobiomarker with a unique fibrosis-related glycoalteration.This biomarker helps to determine liver fibrosis stage in fatty liver disease and viral hepatitis. There is no research to reveal viral infection, fatty liver and liver fibrosis in diabetic patients in Mongolia, so far. So it is necessary to study revealing viral infection, fatty liver disease and to determine stages of fibrosis using WFA+-M2BP to screen liver fibrosis in diabetic patients. OBJECTIVE. To identify viral infection, HCV/HBV in patients with diabetes and to compare liver function and diabetes control for diabetic patients with liver disease. METHODS. We collect data from 25thNovember, 2015 to October of 2016. We got permission of research from the patients by handwriting signature who diagnosed Diabetes mellitus in National University Hospital. Haemotology, biochemistry test, coagulogramm, immunology test are evaluated in 415 patients in clinical laboratory of National university hospital. By the objective, the diabetes patients with viral hepatitis will attend to second step of research. We used HISCL 5000 apparatus of Sysmex Japan to do immunology tests. Also we use SPSS 19.0 and EXCEL program. RESULT. There were 294 patients and by WHO classification of ages 20-29 aged patient (n=4), 30-39 aged(n=19), 40-49 aged(n=65), 50-59 aged(n=126), 60-70 aged(n=48), over 70 aged(n=14)or 53.24±9.43. 146 patients are male,148 patients are female.By BMI 29.9±1.14.By blood test, thrombocytes counted 256.6±11.7;in coagulogramm the prothrombin time was 111.7±31.4; in biochemistry test total bilirubin 16.46±10.6; AP 364.7±192.3;AST 35.7±45.7; ALT 42.8±45.5; GGT 86.53±123.4;albumin 42.06±23.95;total cholesterol 6.04±2.47;triglyceride 5.72±34.5;HbA1c 8.83±5.92; in immunology testsanti-HCV 29.37±18.87 (n=58); HBsAg 590.134±1013.7 (n=23); M2BP (COI) counted 2.24±2.19. CONCLUSION. There were 58 diabetic patients with C viral infection and 23 diabetic patients with B viral infection. By WFA+-M2BP glycobiomarker, we found that diabetic patients with viral hepatitis has more liver fibrosis.

Background: The collagen fibrillar framework of the liver is presumed not only to mechanically support the tissue, but also to form a microenvironment for hepatocytes and cells in the Disse’s space. It has long been considered that the collagen fibers in the liver provide the endothelial cells of the hepatic sinusoids with a scaffold, while their excessive accumulation in the hepatic parenchyma is associated with the chronicity of liver diseases and the development of cirrhosis. Purpose: To define detailed organization of the collagen fibrils of the liver. Materials and methods: Collagen fibrils were taken from the livers of 5 male and female cadavers who died not from liver diseases at the ages from 35 to 55. Light microscopy of hematoxylin-eosin stained liver tissues confirmed that the materials had no significant pathological changes. The collagen fibrils of the liver were extracted and observed according to our cell-maceration/SEM method. Results: The alkali-water maceration of the liver at room temperature consistently removed all of the cellular elements with their basal lamina, and left collagen fibrils in their natural locations and shapes, thus revealing the continuum of the collagen fibrillar network under the SEM. The diameter of collagen fibrils was around 57 nm. The collagen fibrillar layer of the liver capsule was about 70- 100 µm in thickness. The capsular layer consisted of collagen fibrillar bundles (0.2-4µm in diameter) of densely packed parallel collagen fibrils. Deriving from between such bundles were many collagen fibrils, they wound independently for some distance and then again fused with the bundles. There were many bundles of collagen fibrils that connected the capsular collagen layer to the collagen fibrillar sheaths for housing the hepatic sinusoids. There were no collagen fibrillar septa delimiting the hepatic lobules, instead, collagen fibrillar sheaths for the sinusoids were continuum throughout the liver. Normal human livers contained 12.0 mg of collagen fibrils/g wet tissue. Both type I and III collagens have been demonstrated immunehistochemically in normal liver.

Background: Academician Ragchaa B made a profound contribution to the foundation and development of internal medicine in Mongolia. He was among the first graduates of the Faculty of Medicine at the National University of Mongolia, becoming the country’s first internal medicine physician, educator, and researcher in the field of internal pathology. Ragchaa B achieved numerous pioneering roles, including being, the first Mongolian head of the Department of Internal Medicine, and the first professor in this specialty. His extensive contributions helped establish and advance internal medicine and medical education in Mongolia. Aim: The aim of this study is to analyze the scholarly works of Academician Ragchaa Byambajav to explore and disseminate his valuable contributions to the foundation and development of the science of “Internal Medicine Diagnostics” and hepatology Materials and Methods: This study utilized a documentary research method to analyze the contributions of the distinguished scholar, Academician Ragchaa B. The research involved a comprehensive review of his published books, textbooks, scientific articles, presentations, and archival materials. Additionally, the study examined references to Ragchaa B in books, newspapers, and collected written and oral recollections from his former students across different generations. Through systematic analysis of these sources, the study aimed to provide an in-depth understanding of his academic and professional legacy. Results: Academician Ragchaa B, during his teaching career, graduated approximately 4,000 medical professionals and produced a total of 156 scholarly works. Drawing from his vast knowledge, skills, and experience, he became renowned for writing the textbooks “Clinical Aspects of Botkin’s Disease” (1969) and “Diagnosis of Internal Diseases” (1970), earning recognition as the first scholar to author a Mongolian medical textbook. In 1977, Professor Ragchaa B consolidated his research on liver diseases and authored the monograph “Clinical Aspects of Botkin’s Disease,” which led to him earning the Doctor of Medical Sciences (Sc.D.) degree. Conclusion 1. Academician Ragchaa Byambajav was a distinguished scholar who made significant contributions to the science of internal medicine diagnostics. He served as the first Mongolian head of the “Department of Internal Medicine Diagnostics” in 1953, teaching for over 50 years and acting as a consultant in internal medicine for more than 40 years. 2. He directed research on viral hepatitis and authored the monograph “Clinical Aspects of Botkin’s Disease”, which led to his achievement of a Doctor of Medical Sciences degree in 1977. This accomplishment established him as the first specialized researcher and educator in hepatology in Mongolia.