Objective To characterize the major physiological and biochemical indexes of rabbits introduced to Tibet plateau,to provide the essential reference in experimental studies on rabbits,and to serve the relevant clinical treatment,teaching work and scientific research.Methods Twelve serum biochemical parameters of Californian rabbits and Chinese white rabbits introduced to Tibet since 20 years ago were measured with an automatic biochemistry analyzer.Results In the California rabbits,the values of ALB,TP,A/G,CRE,CHOL and LDH were very significantly higher in males than those in females(P<0.01),and ALT,GLU were significantly higher in females(P<0.05).In the Chinese white rabbits,the value of CRE was significantly higher in males than that in females,but the value of LDH was higher than that in males(P<0.01).There were significant differences between the values of AST,TP,ALB and GLO,A/G,GLU,CRE,LDH(P<0.01),and TG and BUN (P<0.05) in Californian and Chinese white rabbits.The values of AST,TP,BUN and LDH were very significantly different (P<0.01),and those of CRE,ALT,GLO and TG were significantly different (P<0.05) between the female Californian and female Chinese rabbits.Conclusion The experimental results obtained in this study will be valuable reference for relevant clinical diagnosis and treatment,teaching Work and scientific researches in Tibet.